Hybridization chain reaction performed on a metal surface as a means of signal amplification in SPR and electrochemical biosensors

Spiga, Fabio Mario; Bonyár, Attila; Ring, Balázs; Onofri, Manuele; Vinelli, Alessandra; Sántha, H.; Guiducci, Carlotta; Zuccheri, Giampaolo

doi:10.1016/j.bios.2013.10.036

Cited by 27 publications

(23 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown above, this biodetection assay is capable of the specific identification and concentration detection of biochemical samples. We can further enhance its sensitivity and resolution by optimizing the performances and utilizing secondary antibodies or nanoparticles to achieve signal amplification [ 21 , 22 , 23 ].…”

Section: Resultsmentioning

confidence: 99%

A Low-Cost and Portable Dual-Channel Fiber Optic Surface Plasmon Resonance System

Liu

Chen

et al. 2017

Sensors

View full text Add to dashboard Cite

A miniaturization and integration dual-channel fiber optic surface plasmon resonance (SPR) system was proposed and demonstrated in this paper. We used a yellow light-emitting diode (LED, peak wavelength 595 nm) and built-in web camera as a light source and detector, respectively. Except for the detection channel, one of the sensors was used as a reference channel to compensate nonspecific binding and physical absorption. We packaged the LED and surface plasmon resonance (SPR) sensors together, which are flexible enough to be applied to mobile devices as a compact and portable system. Experimental results show that the normalized intensity shift and refractive index (RI) of the sample have a good linear relationship in the RI range from 1.328 to 1.348. We used this sensor to monitor the reversible, specific interaction between lectin concanavalin A (Con A) and glycoprotein ribonuclease B (RNase B), which demonstrate its capabilities of specific identification and biochemical samples concentration detection. This sensor system has potential applications in various fields, such as medical diagnosis, public health, food safety, and environment monitoring.

show abstract

Section: Resultsmentioning

confidence: 99%

A Low-Cost and Portable Dual-Channel Fiber Optic Surface Plasmon Resonance System

Liu

Chen

et al. 2017

Sensors

View full text Add to dashboard Cite

show abstract

“…In HCR, selfassembly of two different single strands forming separate hairpins is triggered by a third filament, yielding a long double-stranded structure. This strategy has been used to increase the bound mass in SPR imaging detection of DNA binding [92] and in SERS detection based on gold NPs [93]. Direct access to kinetics is, however, hindered by the cascade of secondary binding events or by the need of multiple steps, and real-time detection is thus a current challenge.…”

Section: Dna-based Amplification Strategiesmentioning

confidence: 99%

Emerging applications of label-free optical biosensors

et al. 2017

View full text Add to dashboard Cite

Abstract:Innovative technical solutions to realize optical biosensors with improved performance are continuously proposed. Progress in material fabrication enables developing novel substrates with enhanced optical responses. At the same time, the increased spectrum of available biomolecular tools, ranging from highly specific receptors to engineered bioconjugated polymers, facilitates the preparation of sensing surfaces with controlled functionality. What remains often unclear is to which extent this continuous innovation provides effective breakthroughs for specific applications. In this review, we address this challenging question for the class of label-free optical biosensors, which can provide a direct signal upon molecular binding without using secondary probes. Label-free biosensors have become a consolidated approach for the characterization and screening of molecular interactions in research laboratories. However, in the last decade, several examples of other applications with high potential impact have been proposed. We review the recent advances in label-free optical biosensing technology by focusing on the potential competitive advantage provided in selected emerging applications, grouped on the basis of the target type. In particular, direct and real-time detection allows the development of simpler, compact, and rapid analytical methods for different kinds of targets, from proteins to DNA and viruses. The lack of secondary interactions facilitates the binding of small-molecule targets and minimizes the perturbation in single-molecule detection. Moreover, the intrinsic versatility of label-free sensing makes it an ideal platform to be integrated with biomolecular machinery with innovative functionality, as in case of the molecular tools provided by DNA nanotechnology.

show abstract

“…Utilizing a custom-built SPRi (surface plasmon resonance imaging) instrument, the group from BME-ETT confirmed a nearly 5x increase in the target binding signal using HCR. This resulted in a detection limit of 100 pM for the target-DNA, which was a specific DNA sequence of Giardia (a known water parasite) [19].…”

Section: Micro-and Nanoscale Materials Characterizationsmentioning

confidence: 99%

BME VIK Annual Research Report on Electrical Engineering and Computer Science 2015

Vajk¹,

Harsányi

Poppe

et al. 2016

Period. Polytech. Elec. Eng. Comp. Sci.

View full text Add to dashboard Cite

PrefaceThroughout László Vajta, Dean of BME VIK János Levendovszky, Deputy Dean of BME VIK Department of Automation and Applied InformaticsDepartment of Automation and Applied Informatics (AUT) is one of the largest and dynamically developing departments at BME with diverse scope competences such as control theory, embedded systems, software modelling, applied software development, Internet of Things, power electronics, mechatronics, and many others. The department's main activities are education, research and development. Training versatile electrical and software engineers with solid practical knowledge is our top priority. Our profile also includes developing high quality software and hardware solutions for industry partners. All of our activities are backed by strong research background.In 2015, the department has organized the 21 st European Wireless (EW) Conference. The EW 2015 conference hosted more than 140 participants from 30 different countries. The 2015 edition of EW was aimed at addressing a key theme on "5G and beyond."The next sections summarize the key research results of the department in year 2015. IoT and Smart CityThe Internet of Things (IoT) is transforming the surrounding physical objects into an ecosystem of information that enriches our everyday life. The IoT represents the convergence of advances in miniaturization, wireless connectivity, and increased data storage, and is driven by various sensors. Application and service development methods and frameworks are required to support the realization of solutions covering data collection, transmission, data processing, analysis, reporting, and advanced querying. Our SensorHUB framework URBMOBI is a mobile environmental sensor that (i) provides temporally and spatially distributed environmental data, (ii) fulfills the need for monitoring at various places without the costs for a large number of fixed measurement stations, (iii) integrates small and precise sensors in a system that can be operated on buses, trams, or other vehicles, (iv) concentrates on urban heat and thermal comfort, and (v) aims at providing climate services and integration with real-time climate models.utilizes the state-of-the-art open source technologies and provides a unified tool chain for IoT related application and ser-URBMOBI project has been worked out between 2013 and 2015 by the following consortium: RWTH Aachen University (Germany), Netherlands Organization for Applied Scientific Research TNO (Netherlands), ARIA Technologies (France), Budapest University of Technology and Economics (Hungary), and Meteorological and Environmental Earth Observation (Italy).The SOLSUN (Sustainable Outdoor Lighting & Sensory Urban Networks) project is about to demonstrate how intelligent city infrastructure can be created in a cost-effective and sustainable way by reusing existing street lighting as the communications backbone. We apply different technologies and methods to reduce energy consumption at the same time as turning streetlights into nodes on a scalable network that is al...

show abstract

Hybridization chain reaction performed on a metal surface as a means of signal amplification in SPR and electrochemical biosensors

Cited by 27 publications

References 32 publications

A Low-Cost and Portable Dual-Channel Fiber Optic Surface Plasmon Resonance System

A Low-Cost and Portable Dual-Channel Fiber Optic Surface Plasmon Resonance System

Emerging applications of label-free optical biosensors

BME VIK Annual Research Report on Electrical Engineering and Computer Science 2015

Contact Info

Product

Resources

About