Hybridization between damselfishes Dascyllus aruanus and D. reticulatus on the Great Barrier Reef

Abstract

Hybridization between damselfishes Dascyllus aruanus and D. reticulatus on the Great Barrier Reef Hybridization among closely related species is relatively common in marine fishes that spawn mid-water. Although at least 81 species of tropical coral-reef fish have been reported to hybridize in nature (primarily Chaetodontidae, Pomacanthidae and Labridae), hybridization is thought to be exceedingly rare among benthic-nesting species that engage in pair spawning, such as the Pomacentridae (Montanari et al. 2016). The Pomacentridae include >385 species, most of which form breeding pairs and nest on the benthos. Yet only four Pomacentridae hybrids have been confirmed based on strong molecular evidence (e.g., Yaakub et al. 2006), and only from areas where one or both species are rare, such as degraded habitats and/or geographic zones of overlap. Here we present both morphological and genetic evidence for the first record of hybridization between Dascyllus aruanus and D. reticulatus (Fig. 1). These are small (4-9 cm total length, TL) benthic-nesting fishes that often cohabit small branching coral heads. They display distinct pairing during breeding, vigorously guarding their eggs until hatching. The hybrid was found cohabiting a colony of Pocillopora damicornis with several D. aruanus and D. reticulatus at a depth of 6 m off Lizard Island (14°40¢08 †S, 145°27¢34 †E), Great Barrier Reef, Australia. The hybrid was adult size (6 cm TL) and had coloration and meristic counts intermediate to both D. aruanus and D. reticulatus. Dascyllus aruanus is white with three broad black bars over the head, pectoral fins and anterior to the caudal fin (Fig. 1a). By comparison, D. reticulatus has a tan body and iris, with one thin black bar across the pectoral fins and along the dorsal margin (Fig. 1c). Both species have black pelvic fins, and pale caudal and pectoral fins. The hybrid had a white body with a light spot on the snout, two black bars over the head and pectoral fins, a black bar along the dorsal margin, but no bar across the anterior part of the caudal fin (Fig. 1b). It also had an intermediate number of soft dorsal fin rays and pectoral fin rays (14 and 19, respectively). However, the number of anal fin rays, tubed lateral line scales and gill rakers were identical only to D. reticulatus (14, 18 and 27, respectively). Analysis of a nuclear gene (TMO-4C4) revealed that the hybrid carried haplotypes identical to both parent species with the two lineages distributed evenly within the hybrid genotype. While Pomacentridae hybridization is rarely observed, this record emphasizes the potential for natural hybridization in areas of high abundance and well within geographic distribution limits.

Species integrity, introgression, and genetic variation across a coral reef fish hybrid zone

“…Hybridization was once considered rare in the marine environment (Arnold, 1997); however, a surge of recent studies has challenged these traditional perceptions of hybrid scarcity (Gardner, 1997; Harrison et al., 2017; He, Johansen, Hoey, Pappas, & Berumen, 2019; Johansen et al., 2017; Montanari, Hobbs, Pratchett, Bay, & van Herwerden, 2017; Montanari, Hobbs, Pratchett, & van Herwerden, 2016; Pazmiño et al., 2019; Willis, van Oppen, Miller, Vollmer, & Ayre, 2006). Hybridization is particularly common in coral reef fishes, where largely allopatric sister species hybridize at their biogeographic borders.…”