Hybridization and hybrid detection through molecular markers in finger millet [<i>Eleusine coracana</i>(L.) Gaertn.]

Krishna, Thumadath Palayullaparambil Ajeesh; Maharajan, Theivanayagam; Roch, G. Victor; Ramakrishnan, Muthusamy; Ceasar, S. Antony; Ignacimuthu, S.

doi:10.1080/15427528.2019.1709596

Cited by 25 publications

(13 citation statements)

References 35 publications

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“…Based on the previous report, two contrasting finger millet genotypes, viz., IE-2606 (low-Pi-susceptible) and PR-202 (low-Pi-tolerant) [17], were selected as parents to develop RILs. These two genotypes were used to cross and develop F 1 RILs [49]. Around 100 F 2 RILs were developed from the F 1 RILs following a single-seed descent (SSD) method; all seeds were genetically different, and each seed was maintained as a separate line.…”

Section: Plant Materialsmentioning

confidence: 99%

Identification of QTL Associated with Agro-Morphological and Phosphorus Content Traits in Finger Millet under Differential Phosphorus Supply via Linkage Mapping

et al. 2023

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Finger millet (Eleusine coracana (L.) Gaertn.) is one of the most important cereal crops for people in Asia and Africa. It supplies energy and nutrients for all people, particularly poor people in these continents. Phosphorus (P) is an essential macronutrient for plant growth and grain quality, so an adequate amount of P is required for healthy plant life. There is considerable evidence that P deficiency affects the growths and yields of all cereals, including finger millet. The present study aimed at identifying the quantitative trait loci (QTL) for various agro-morphological and P-content traits under low P (LP) and high P (HP) conditions through linkage mapping. Two cultivars, IE-2606 (low-P-susceptible) and PR-202 (low-P-tolerant) were used to develop 100 recombinant inbred lines (RILs). In total, 55 QTL, associated with various agro-morphological (primary root length (PRL), shoot fresh weight (SFW), root fresh weight (RFW), shoot dry weight (SDW), root dry weight (RDW), and root hair density (RHD)) and P-content traits (inorganic phosphate content in shoots (PiS), inorganic phosphate content in roots (PiR), and total P content in shoots (TPS)) were detected under LP conditions. Similarly, 37 QTL for various agro-morphological (PRL, RFW, SDW, and root hair length (RHL)) and P-content traits (PiS, PiR, TPS, and total P content in roots (TPR)) were detected under HP conditions. Biotic and abiotic stress-responsive candidate genes linked to a few QTL were also identified. The identified QTL associated with agro-morphological and P-content traits in finger millet under P deficiency could be used in breeding programs to develop finger millet with better P use efficiency (PUE). Furthermore, improving finger millet growth and yield under LP soil will help to reduce Pi application and eutrophication, which may help to improve the lives of poor farmers and maintain sustainable environments in Asia and African countries.

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Section: Plant Materialsmentioning

confidence: 99%

Identification of QTL Associated with Agro-Morphological and Phosphorus Content Traits in Finger Millet under Differential Phosphorus Supply via Linkage Mapping

et al. 2023

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“…), pegFinder web tool (http://pegfinder.sidichenlab.org/) that predicts target site and also provides oligo sequence for cloning. [ 21 ]…”

Section: The Prime Editing System Work Through Three Different Compon...mentioning

confidence: 99%

“…Conventional plant‐breeding techniques were applied to improve the traits of crops to overcome these stresses, but they were labor and time‐intensive. [ 21,22 ] Later, molecular‐marker assisted breeding (MAB) was used to improve the efficiency of conventional breeding, [ 23,24 ] but this tool also met with too many limitations with labor‐intensive steps. [ 22 ] In the past decades, genetic engineering was also utilized to transfer novel genes from other sources to plants to improve traits and yields under adverse climatic conditions.…”

Section: Prime Editing System Improves Precise Base Editing In Plantsmentioning

confidence: 99%

Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects

Hillary

Ceasar

2022

BioEssays

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Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPRassociated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas-derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have been introduced for engineering the genomes of diverse organisms. The recently introduced prime editing system offers precise editing without many off-target effects than traditional CRISPRbased systems. Many researchers have successfully applied this gene-editing toolbox in diverse systems for various genome-editing applications. This review presents the mechanism of prime editing and summarizes the details of the prime editing system applied in plants and mammalian cells for precise genome editing. We also discuss the advantages, limitations, and potential future applications of prime editing in these systems. This review enables the researcher to gain knowledge on prime editing tools and their potential applications in plants and mammalian cells.

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“…Confirmation of cross success and discrimination between parent genotypes and hybrids is essential for genetic breeding. During breeding, the obtained hybrids are prone to contamination by outcrossing with foreign pollen or physical admixtures (Carvalho, 2008;Krishna et al, 2020). In autogamous species, such as C. arabica, self-pollination is common before controlled outcrossing, preventing the transfer of desired traits in progenies.…”

Section: Hybrid Identification By Molecular Markersmentioning

confidence: 99%

Combining Ability and Molecular Marker Approach Identified Genetic Resources to Improve Agronomic Performance in Coffea arabica Breeding

Medeiros

Caixeta

Oliveira

et al. 2021

Front. Sustain. Food Syst.

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Plant breeding aims to develop cultivars with good agronomic traits through gene recombination and elite genotype selection. To support Coffea arabica breeding programs and assist parent selection, molecular characterization, genetic diversity (GD) analyses, and circulating diallel studies were strategically integrated to develop new cultivars. Molecular markers were used to assess the GD of 76 candidate parents and verify the crossing of potential F1 hybrids. Based on the complementary agronomic traits and genetic distance, eight elite parents were selected for circulating diallel analysis. The parents and 12 hybrids were evaluated based on 10 morpho-agronomic traits. For each trait, the effects of general and specific combining abilities, as well as the averages of the parents, hybrids, and predicted hybrids, were estimated. Crosses that maximize the genetic gains for the main agronomic traits of C. arabica were identified. Joint analysis of phenotypic and molecular data was used to estimate the correlation between molecular GD, phenotypic diversity (PD), phenotypic mean, and combining ability. The selection of parents that optimize the allele combination for the important traits of C. arabica is discussed in detail.

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Hybridization and hybrid detection through molecular markers in finger millet [Eleusine coracana(L.) Gaertn.]

Cited by 25 publications

References 35 publications

Identification of QTL Associated with Agro-Morphological and Phosphorus Content Traits in Finger Millet under Differential Phosphorus Supply via Linkage Mapping

Identification of QTL Associated with Agro-Morphological and Phosphorus Content Traits in Finger Millet under Differential Phosphorus Supply via Linkage Mapping

Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects

Combining Ability and Molecular Marker Approach Identified Genetic Resources to Improve Agronomic Performance in Coffea arabica Breeding

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