Hybridization and asymmetric introgression between Tetragonisca angustula and Tetragonisca fiebrigi

Francisco, Flávio de Oliveira; Santiago, Leandro Rodrigues; Brito, Rute Magalhães; Oldroyd, Benjamin P.; Arias, Maria Cristina

doi:10.1007/s13592-013-0224-7

Cited by 28 publications

(38 citation statements)

References 54 publications

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“…Stuchi et al (2012) conducted an esterase biochemical characterization (as described by Camargo and Pedro, 2007) that suggested that T. angustula and T. fiebrigiare different species, because esterases 1, 2, and 4 were found in T. fiebrigi workers and esterases 3 and 4 were found in T. angustula workers. Francisco et al (2014) confirmed the existence of hybrids between Tetragonisca by sequencing their mtDNA regions and conducting microsatellite analysis, and suggest that these bees are subspecies.…”

Section: Introductionmentioning

confidence: 56%

“…Bees that exhibited variations in the color of their mesepisterna were also found, suggesting a cross between the species that would generate hybrids (Castanheira and Contel, 2005). Francisco et al (2014) and Koling and Moretto (2010) observed these hybrid bees, and suggested that they could be subspecies.…”

Section: Discussionmentioning

confidence: 96%

“…This confirmed that PCR-RFLP markers could be used for the identification of matrilineages. Francisco et al (2014) conducted a study on T. angustula and T. fiebrigi using microsatellite markers and mtDNA sequencing, and observed crosses between T. fiebrigi males and T. angustula females. The authors suggested that the crosses occurred in areas where there was a predominance of T. angustula, and T. fiebrigi probably produced a greater number of males.…”

Section: Discussionmentioning

confidence: 99%

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Matrilineage differentiation of the genus Tetragonisca using mitochondrial DNA markers and the polymerase chain reaction-restriction fragment length polymorphism technique

Gigliolli¹,

Bronzato²,

Moreira³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The Meliponinae are important pollinators of plant species, and one of the most managed species is Tetragonisca angustula. Initially, two subspecies were identified in T. angustula: T. angustula angustula and T. angustula fiebrigi. Subsequently, T. a. fiebrigi was considered a species, based on the coloration of its mesepisternum. The objective of the present study was to obtain genetic markers that could differentiate the two species by amplifying regions of mitochondrial DNA and conducting polymerase chain reaction-restriction fragment length polymorphism analysis. Worker bees were collected in three Brazilian states: Paraná (Maringá, Altônia, and Foz do Iguaçu), São Paulo (Dracena, São Carlos, and Santa Cruz do Rio Pardo), and Rondônia (Ariquemes). Ten pairs of insect heterologous primers were tested and four were used (primer pair 1, ND2 and COI; primer pair 2, COI; primer pair 8, 16S and 12S; and primer pair 9, COII). For the restriction analysis, 13 enzymes were tested: EcoRI, EcoRV, HindIII, HinfI, RsaI, PstI, XbaI, HaeIII, ClaI, XhoI, BglII, PvuII, and ScaI. Markers were obtained (primer pair 8 cleaved with EcoRV and XbaI and primer pair 9 cleaved with HaeIII, RsaI, and XbaI) that enabled matrilineage identification in the nests studied, which confirmed that hybridization could occur between both Tetragonisca species. The beginning of speciation was probably recent, and secondary contact has resulted in crosses between T. angustula females and T. fiebrigi males. Because of this hybridization, it would be appropriate to consider them as two subspecies of T. angustula.

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Section: Introductionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

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Matrilineage differentiation of the genus Tetragonisca using mitochondrial DNA markers and the polymerase chain reaction-restriction fragment length polymorphism technique

Gigliolli¹,

Bronzato²,

Moreira³

et al. 2015

Genet. Mol. Res.

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“…PCR and genotyping details for each locus are described elsewhere (Francisco et al 2011(Francisco et al , 2014. The program MICRO-CHECKER 2.2.3 (Van Oosterhout et al 2004) was used to verify the presence of scoring errors.…”

Section: Genotypingmentioning

confidence: 99%

“…PCR conditions, electrophoresis, gel staining and purification followed a previously published procedure (Francisco et al 2014). Purified amplicons were sequenced (Macrogen, South Korea) using the primers mtD06 and mtD26 for MT-COI and MT-CYB, respectively.…”

Section: Sequencingmentioning

confidence: 99%

Genetic variability in captive populations of the stingless bee Tetragonisca angustula

et al. 2016

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Low genetic variability has normally been considered a consequence of animal husbandry and a major contributing factor to declining bee populations. Here, we performed a molecular analysis of captive and wild populations of the stingless bee Tetragonisca angustula, one of the most commonly kept species across South America. Microsatellite analyses showed similar genetic variability between wild and captive populations However, captive populations showed lower mitochondrial genetic variability. Male-mediated gene flow, transport and division of nests are suggested as the most probable explanations for the observed patterns of genetic structure. We conclude that increasing the number of colonies kept through nest divisions does not negatively affect nuclear genetic variability, which seems to be maintained by small-scale male dispersal and human-mediated nest transport. However, the transport of nests from distant localities should be practiced with caution given the high genetic differentiation observed between samples from western and eastern areas. The high genetic structure verified is the result of a long-term evolutionary process, and bees from distant localities may represent unique evolutionary lineages.

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Agricultural insect hybridization and implications for pest management

Corrêa

Cordeiro

Omoto

2019

Pest Management Science

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Biological invasions, the expansion of agricultural frontiers, and climate change favor encounters of divergent lineages of animals and plants, increasing the likelihood of hybridization. However, hybridization of insect species and its consequences for agroecosystems have not received sufficient attention. Gene exchange between distinct and distant genetic pools can improve the survival and reproduction of insect pests, and threaten beneficial insects in disturbed agricultural environments.Hybridization may be the underlying explanation for the recurrent pest outbreaks and control failures in putative hybrid zones, as suspected for bollworm, corn borer, whiteflies, and stink bugs. Reliable predictions of the types of changes that can be expected in pest insect genomes and fitness, and of their impacts on the fate of species and populations remain elusive. Typical steps in pest management, such as insect identification, pest monitoring, and control are likely affected by gene flow and adaptive introgression mediated by hybridization, and we do not have ways to respond to or mitigate the problem. To address the adverse effects of farming intensification and global trade, we must ensure that current integrated pest management programs incorporate up-to-date monitoring and diagnostic tools. The rapid identification of hybrids, quantification of levels of introgression, and in-depth knowledge of what genes have been transferred may help to explain and predict insect population outbreaks and control failures in the future.

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Hybridization and asymmetric introgression between Tetragonisca angustula and Tetragonisca fiebrigi

Cited by 28 publications

References 54 publications

Matrilineage differentiation of the genus Tetragonisca using mitochondrial DNA markers and the polymerase chain reaction-restriction fragment length polymorphism technique

Matrilineage differentiation of the genus Tetragonisca using mitochondrial DNA markers and the polymerase chain reaction-restriction fragment length polymorphism technique

Genetic variability in captive populations of the stingless bee Tetragonisca angustula

Agricultural insect hybridization and implications for pest management

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