Background The purpose of this study is to evaluate the satisfaction of surgeons and postgraduate trainees on 3D heads up ophthalmic surgery compared to traditional microscopic surgery in terms of working and educational aspect.Methods This was a validated questionnaire-based study. This study was conducted at a tertiary referral and postgraduate teaching hospital in Kuala Lumpur, Malaysia over a one-month period during which the Ngenuity viewing system was demonstrated in the Ophthalmology operating theatre. All surgeons and trainees exposed to the viewing system were recruited for the study which involved answering an online questionnaire comprising the components of visualization, physical, ease of use, teaching and learning components and a question on satisfaction. This questionnaire is designed to evaluate the perception of the students and surgeons regarding learning-teaching methodology and benefits gained by them comparing between 3D heads up system and traditional microscopic surgery.Results All seven surgeons and 33 postgraduate students responded to the survey. A total of 32 cases were operated with the 3D system, which consisted of phacoemulsification, pterygium surgery, glaucoma procedures, cornea and vitreoretinal operations. There was no significant difference reported by the surgeons in all specific components (p>0.05, paired t-test). Postgraduate trainees reported better experience with 3D heads up surgery compared to traditional microscopic operation in terms of the illumination (p=0.008) and manoeuvrability (p= 0.01), physical factors such as glare p=0.037), eye strain (p=0.008), neck and upper back pain (p=0.000), lower back pain (p=0.019) and for all subcomponents of teaching and learning. Both surgeons and postgraduate trainees were more satisfied with 3D heads up system in general than traditional microscopic surgery (p=0.047 and p=0.009 respectively).Conclusions 3D heads up surgery and viewing is preferred to traditional microscopic surgery viewing particularly by postgraduate trainees. The overall satisfaction with this viewing system is also higher among surgeons and trainees than traditional microscope viewing.Trial Registration: Reference number: UKM PPI/111/8/JEP-2019-724 (UKM Research Ethics Committee) Date: 6th November 2019