Hybrid sterility and evolution in Hawaiian Drosophila: differential gene and allele-specific expression analysis of backcross males

Brill, Eva; Kang, Lin; Michalak, Katarzyna; Michalak, Pawel; Price, Daniel O.

doi:10.1038/hdy.2016.31

Cited by 21 publications

(18 citation statements)

References 47 publications

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“…RNA-seq data for D. silvestris, D. heteroneura, and D. planitibia were downloaded from NCBI Gene Expression Omnibus (GEO, www.ncbi. nlm.nih.gov/geo) under accession number GSE80124 and from our previous study [Brill et al, 2016]. Adapters were removed from the raw sequencing reads.…”

Section: Gene Prediction and Annotationmentioning

confidence: 99%

Mapping Genomic Scaffolds to Chromosomes Using Laser Capture Microdissection in Application to Hawaiian Picture-Winged Drosophila

Kang

George²,

Price³

et al. 2017

Cytogenet Genome Res

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successfully assigned to chromosome arms. Even though the scaffolds are not ordered within a chromosome, the fastgenerated chromosome information allows for chromosome-related analyses after genome assembling. We utilize this new information to test the faster-X evolution effect for the first time in these Hawaiian picture-winged Drosophila species. © 2017 S. Karger AG, Basel Along with the rapid development of massively parallel sequencing, or next-generation sequencing (NGS), DNA sequencing capabilities grow exponentially. This allows researchers to perform de novo genome assemblies for thousands of species, from viruses and insects to mammals (e.g., Genome 10K, i5K [Genome 10K Community of Scientists, 2009; i5K Consortium, 2013]). However, the complexity of the assembly task using short reads still poses a major challenge. Genome assemblies are hierarchical, and a typical de novo assembly process, especially for large eukaryotic genomes, often ends at the level of scaffolds, assembled from shorter as- KeywordsChromosome mapping · Hawaiian Drosophila · Laser capture · Microdissection Abstract Next-generation sequencing technologies have led to a decreased cost and an increased throughput in genome sequencing. Yet, many genome assemblies based on short sequencing reads have been assembled only to the scaffold level due to the lack of sufficient chromosome mapping information. Traditional ways of mapping scaffolds to chromosomes require a large amount of laboratory work and time to generate genetic and/or physical maps. To address this problem, we conducted a rapid technique which uses laser capture microdissection and enables mapping scaffolds of de novo genome assemblies directly to chromosomes in Hawaiian picture-winged Drosophila . We isolated and sequenced intact chromosome arms from larvae of D. differens. By mapping the reads of each chromosome to the recently assembled scaffolds from 3 Hawaiian picturewinged Drosophila species, at least 67% of the scaffolds were

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Section: Gene Prediction and Annotationmentioning

confidence: 99%

Mapping Genomic Scaffolds to Chromosomes Using Laser Capture Microdissection in Application to Hawaiian Picture-Winged Drosophila

Kang

George²,

Price³

et al. 2017

Cytogenet Genome Res

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“…In many eukaryotic organisms, especially Drosophila, hybrid male sterility is a key event of post-zygotic reproductive isolating mechanism, and, our results of experimental hybridization between these two sibling species provides the evidence of preliminary post-zygotic reproductive isolation. In the present study, post-zygotic isolation might be the result of distorted interaction of genetic combination of the parental lineages that, although functional in their normal genetic backgrounds, reduce fitness when recombined in hybrids (Rundle, Nagel, Boughman, & Schluter, 2000;Burton, Ellison, & Harrison, 2006;Rogers & Bernatchez, 2006;Gow, Peichel, & Taylor, 2007). Several genes are involved in the expression of this complex and polygenic trait (Gomes & Civetta, 2015).…”

Section: Discussionmentioning

confidence: 81%

“…In a long history of evolutionary biology, hybrid male sterility was studied extensively by using Drosophila as a model because Drosophila is particularly well suited to study such kind of reproductive barriers, as species within this genus are highly variable in degree of reproductive isolation from noninterbreeding species to hybridizing species (Yukilevich & True, 2008;Civetta & Gaudreau, 2015;Brill, Kang, K. Michalak, P. Michalak, & Price, 2016). Therefore, in the present study, we studied hybrid sterility between these two sibling species to understand the exact status of post-zygotic isolation by means of larval activity.…”

Section: Discussionmentioning

confidence: 99%

Evidence for Initiation of Post-Zygotic Reproductive Isolation between Drosophila ananassae and D. pallidosa as Indicated by Reduction in the Fertility of Hybrid Males

Singh¹,

Singh²

2020

IJB

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There are several barriers to preclude the gene flow between diverging populations. On the basis of their temporal nature, these can be broadly categorized into two forms: pre- and post-zygotic. Post-zygotic reproductive isolation can manifest in the form of reductions in hybrid fertility. Keeping this fact in view, in the present study, we studied sterility in hybrids of D. ananassae and D. pallidosa. Surprisingly a distinguishable pattern of infertility was found in the hybrids. This pattern, referred to as Haldane’s rule, is often observed in hybrids of recently diverged populations or species. Reduction in the fertility of hybrids provides the clue of incipient kind of post-zygotic reproductive isolation in these two sibling species. This is the first report of hybrid sterility in this species pair. However, hybrid sterility is not very prominent especially when compared to that of other species pairs with the similar divergence time. Thus, on the basis of our results, we conclude that either sexual isolation between these sibling species is sufficient and does not require the aid of post-zygotic isolation to preclude gene flow or rate of divergence between D. ananassae and D. pallidosa is very slow in comparison to other species pair or even races of some species.

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“…Despite the clear importance of changes in gene expression for phenotypic evolution (Wittkopp, 2013), empirical support for regulatory divergence as a general driver of hybrid incompatibilities is mixed. While many studies have uncovered pervasive gene misexpression in sterile hybrids (Michalak & Noor, 2003; Ranz et al , 2004; Haerty & Singh, 2006; Malone et al , 2007; Coolon et al , 2014; Brill et al , 2016; Mack et al , 2016), others have found no association between patterns of gene expression and hybrid dysfunction (Barbash & Lorigan, 2007; Wei et al , 2014; Guerrero et al , 2016). Even when sterile or inviable hybrids do show misexpression, it is usually difficult to determine which is cause and which is effect.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide misexpression associated with hybrid sterility inMimulus(monkeyflower)

Kerwin

2019

Preprint

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Divergence in gene expression regulation is common between closely related species and may give rise to incompatibilities in their hybrid progeny. In this study, we investigated the relationship between regulatory evolution within species and reproductive isolation between species. We focused on a well-studied case of hybrid sterility between Mimulus guttatus and M. nasutus, two closely related yellow monkeyflower species, that is caused by two epistatic loci, hybrid male sterility 1 (hms1) and hybrid male sterility 2 (hms2). We quantified and compared global transcript abundance across male and female reproductive tissues (i.e. stamens and carpels) of M. guttatus and M. nasutus, as well as sterile and fertile progeny from an advanced M. nasutus-M. guttatus introgression line that carries the hms1-hms2 incompatibility. We observed substantial variation in transcript abundance between M. guttatus and M. nasutus, including distinct but overlapping patterns of tissue-biased expression, providing evidence for regulatory divergence between these species. Furthermore, we found pervasive genome-wide misexpression exclusively associated with hybrid sterility – only observed in the affected tissues (i.e. stamens) of sterile introgression hybrids. Examining patterns of allele-specific expression in sterile and fertile hybrids, we found evidence of cis- and trans- regulatory divergence, as well as cis-trans compensatory evolution (likely to be driven by stabilizing selection). However, regulatory divergence does not appear to cause misexpression in sterile hybrids, which instead likely manifests as a downstream consequence of sterility itself.

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Hybrid sterility and evolution in Hawaiian Drosophila: differential gene and allele-specific expression analysis of backcross males

Cited by 21 publications

References 47 publications

Mapping Genomic Scaffolds to Chromosomes Using Laser Capture Microdissection in Application to Hawaiian Picture-Winged Drosophila

Mapping Genomic Scaffolds to Chromosomes Using Laser Capture Microdissection in Application to Hawaiian Picture-Winged Drosophila

Evidence for Initiation of Post-Zygotic Reproductive Isolation between Drosophila ananassae and D. pallidosa as Indicated by Reduction in the Fertility of Hybrid Males

Genome-wide misexpression associated with hybrid sterility inMimulus(monkeyflower)

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