Hybrid scaffolds of gelatin–siloxane releasing stromal derived factor‐1 effective for cell recruitment

Abstract

Scaffolds with the capacity to deliver signaling molecules are attractive for bone regeneration. Here, we developed bioactive siloxane-gelatin hybrid scaffolds via a sol gel process containing stromal derived factor-1 (SDF-1) to recruit osteoprogenitor/stem cells. The process was undertaken under room temperature aqueous conditions, which enabled therapeutic molecules to be effectively incorporated. After the sol-gel reaction and lyophilization process, well-crosslinked hybrid scaffolds were obtained with porosities of 80-90%. Dynamic mechanical analysis of the hybrid scaffolds showed significant improvement in storage modulus values (from 10 to 110 kPa) with increasing siloxane content. The protein release capacity of the scaffolds was investigated using a model protein cytochrome C (cyto C). The cyto C safely loaded onto the scaffolds exhibited, except the initial burst of 30% within a day, highly sustainable release, with approximately 70-80% of the loading amount for up to 4 weeks. Target molecule SDF-1 was loaded and released from the scaffolds, and the effects on the homing of mesenchymal stem cell were studied. Results demonstrated significant enhancement in the migration of cells to the SDF-1 loaded scaffolds. Taken together, the developed hybrid scaffolds are considered to be useful in loading and delivering signaling molecules such as SDF-1 to recruit osteoprogenitor /mesenchymal stem cells in the bone regeneration process.

Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells

“…As aforementioned, SDF-1 is a powerful chemokine, which directly induces cell migration in various cell types including NSC, MSC and so on. In our previous study, SDF-1 loaded scaffolds significantly enhanced cell migration (17). Similarly, SDF-1 significantly enhanced the migration of PC-12 cells in the present study.…”

“…Construction of expression plasmids. Recombinant SDF-1 protein was constructed as described previously (17). Briefly, the human SDF-1 sequence was amplified by PCR (Perkin Elmer, Inc.) using primers flanked with restriction sites for XhoI and KpnI (forward, 5'-XhoI-AACCTCGAGAGCGAT GGCAAACCGGTG-3'; reverse, 5'-KpnI-GTTGGTACCTTA TTTGTTCAGCGCT-3').…”

“…In the present study, it was hypothesized that a recombinant SDF-1 protein might induce neuronal differentiation of PC-12 cells. A recombinant SDF-1 protein constructed in our previous study was isolated and purified in Escherichia coli (E. coli) (17). Cell toxicity and the proliferative effect of SDF-1 protein in PC-12 cells were measured.…”

Periodontal regeneration via chemoattractive constructs

“…Similar result had also been reported by another research group (Huang, Gronthos, & Shi, 2009). However, other studies using constructs with burst release of SDF-1α in the early stage demonstrated successfully the recruitment of BMSCs (Dashnyam et al, 2014;He, Ma, & Jabbari, 2010;Ji et al, 2013).…”

“…Similar result had also been reported by another research group (Huang, Gronthos, & Shi, ). However, other studies using constructs with burst release of SDF‐1α in the early stage demonstrated successfully the recruitment of BMSCs (Dashnyam et al., ; He, Ma, & Jabbari, ; Ji et al., ). Therefore, further investigation is needed to confirm the recruitment of GFP‐positive rat BMSCs in the periodontal defect, and higher dose of SDF‐1α might be considered.…”