Hybrid Periodic Mesoporous Organosilicas (PMO-SBA-16): A Support for Immobilization of <scp>d</scp>-Amino Acid Oxidase and Glutaryl-7-amino Cephalosporanic Acid Acylase Enzymes

Nohair, Bendaoud; Thao, Phan Thi Hong; Vu, Nguyen; Tien, Phi Quyet; Phuong, Dang Tuyet; Hy, Le Gia; Kaliaguine, Serge

doi:10.1021/jp2116998

Cited by 19 publications

(9 citation statements)

References 56 publications

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“…The morphologies of the MIP and NIP after removal of template are shown in figure 2 below. The images of the MIP illustrate that the PMO-MIP had almost cubic particle morphology similar to the prepared BTEB-SBA 16 reported by Nohair and his colleagues 15 . Zang and companions concluded that large caged cubic PMO could be prepared easily by using F127 in the presence of acetone or tetrahydrofuran instead of ethanol which is why it is believed that the polymer had large domains of well-ordered cage like mesostructure 16 .…”

Section: Semsupporting

confidence: 76%

Synthesis and Characterization of a Periodic Mesoporous Organosilica Molecular Imprinted Polymer for Removal of DDT

Sithole¹,

Thwala²,

Bwembya³

2019

Journal of Harmonized Research in Applied Science

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Section: Semsupporting

confidence: 76%

Synthesis and Characterization of a Periodic Mesoporous Organosilica Molecular Imprinted Polymer for Removal of DDT

Sithole¹,

Thwala²,

Bwembya³

2019

Journal of Harmonized Research in Applied Science

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“…Zhu and coworkers 96 synthesized a novel hierarchical PMO monolith using N,Sbis [3-(triethoxysilyl)propyl]-carbamothioic acid to fabricate a highly efficient enzyme immobilizer. Kaliaguine and co-workers 97 examined the adsorption and stability of D-amino acid oxidase and glutaryl-7-amino cephalosporanic acid acylase using two different types of PMOs: PMO-SBA-16 synthesized from 1,2-bis(trimethoxysilyl)ethane (BTME) and 1,4-bis(triethoxysilyl)benzene. Very high loading (68.8 mg g À1 for D-amino acid oxidase, 51.9 mg g À1 for glutaryl-7-amino cephalosporanic acid acylase), specific enzymatic activities and stabilities were obtained after proper optimization of the mesopore structure and morphology.…”

Section: Pmos For Advanced Applications Ss Park Et Almentioning

confidence: 99%

Periodic mesoporous organosilicas for advanced applications

2014

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In 1999, three groups independently developed a novel class of organic-inorganic nanocomposites known as periodic mesoporous organosilicas (PMOs). The organic functional groups in the frameworks of these solids allow tuning of the surface properties and modification of the bulk properties of the material. This paper provides a comprehensive overview of PMOs and discusses their different functionalities, morphology and applications, such as catalysis, drug delivery, sensing, optics, electronic devices, environmental applications (gas sensing and gas adsorption), biomolecule adsorption and chromatography for which PMOs have been used over the past 5 years.

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“…The procedure for immobilization of rGLA on nanoporous materials was performed as follows method Nohair et al [8].…”

Section: Immobilization Of Rgla On Nanomaterialsmentioning

confidence: 99%

Expression of Glutaryl7-Aminocephalosporanic Acid Acylase in Escherichia Coli Bl21(de3) and Immobilization of Recombinant Enzyme on Nanoporous Materials

Hanh

2018

JST

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The synthesis of 7-ACA from cephalosporin C (CPC) by a two-step bioconversion using D-amino acid oxidase (DAAO) and glutaryl 7-ACA acylase (GLA) has been effectively and largely applied in pharmaceutical industry. In this study, the gene gla coding for 720-amino acid GLA from plasmid pUC57::gla was analyzed and successfully inserted into vector pET22b(+) to form expression vector pET22b(+)::gla. The newly constructed expression vector pET22b(+)::gla was cloned and then transformed into Escherichia coli BL21(DE3) to generate recombinant strain E. coli BL21(DE3)[pET22b(+)::gla]. The suitable conditions for expression of gla gene were in LB medium at 30 o C and induced by 0.4 mM of Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 3 hours. Under the chosen culturing parameters, expression of gla gene by E. coli BL21(DE3)/[pET22b(+)::gla] resulted in a recombinant GLA (rGLA) with molecular weight of 83 kDa and catalytic activity of 2.7 U/mg of total protein. Experimental research on immobilization of rGLA onto ten nanoporous materials were showed that, SBA-15 was the best one for immobilization of rGLA, reaching activity of immobilized enzyme of 22.2 U/g matrix. Furthermore, optimal conditions of procedure for immobilizing rGLA on nanomaterials (SBA-15) were determined as follows: temperature is 25 C, pH7.0 and immobilization time -60 minutes. Therefore the results reported in this study revealed the successfully heterologous expression of GLA in recombinant E. coli and potential immobilization of enzyme on inorganic nano-materials.

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Hybrid Periodic Mesoporous Organosilicas (PMO-SBA-16): A Support for Immobilization of d-Amino Acid Oxidase and Glutaryl-7-amino Cephalosporanic Acid Acylase Enzymes

Cited by 19 publications

References 56 publications

Synthesis and Characterization of a Periodic Mesoporous Organosilica Molecular Imprinted Polymer for Removal of DDT

Synthesis and Characterization of a Periodic Mesoporous Organosilica Molecular Imprinted Polymer for Removal of DDT

Periodic mesoporous organosilicas for advanced applications

Expression of Glutaryl7-Aminocephalosporanic Acid Acylase in Escherichia Coli Bl21(de3) and Immobilization of Recombinant Enzyme on Nanoporous Materials

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