Hybrid observation of crystal growth using laser confocal microscope with atomic force microscope

Yanagiya, Shin-ichiro; Goto, Nobuo

doi:10.1016/j.jcrysgro.2010.08.035

Cited by 10 publications

(15 citation statements)

References 12 publications

(13 reference statements)

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“…An Ar ion laser of 488 nm or a He-Ne laser of 543 nm was used in the LCM-DIM observations. As described in our previous study [7], the unfavorable laser for LCM-DIM observation, which was used in the AFM control, was filtered by a mirror and filters in the confocal unit. The time course of the crystal growth was observed using LCM-DIM with AFM scanning.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous studies [7,8], we observed a potassium dihydrogen phosphate (KDP) crystal surface and its growth using a transmission optical microscope, LCM-DIM, and AFM. Using this integrated instrumental setup, we were able to approach an AFM cantilever tip to crystals several-tens of microns in size and observe the steps with measuring the height using two-beam interference microscopy [7].…”

Section: Introductionmentioning

confidence: 99%

“…Using this integrated instrumental setup, we were able to approach an AFM cantilever tip to crystals several-tens of microns in size and observe the steps with measuring the height using two-beam interference microscopy [7]. We also demonstrated the measurement of the refractive index of a small volume of water [8].…”

Section: Introductionmentioning

confidence: 99%

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Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal

Yanagiya¹,

Goto²

2012

JSEMAT

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We demonstrated the in-situ observation of a moving atomic force microscope (AFM) cantilever using a laser confocal microscope combined with a differential interference microscope (LCM-DIM). The AFM cantilever scanned or indented the {110} surface of a hen egg-white lysozyme crystal in a supersaturated solution. Using a soft cantilever, we could observe the step growth with high time resolution by LCM-DIM and perform quantitative measurements of the step height by AFM simultaneously. In addition, a hard cantilever was used with LCM-DIM to observe the dynamics of crystal surface scratching and indentation. In the supersaturated solution, the small steps generated from the scratched line aggregated to macro steps, and subsequently flattened the surface.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal

Yanagiya¹,

Goto²

2012

JSEMAT

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“…We have studied the observation of surface dynamics of transparent materials in an aqueous solution [10,11] and the measurements of refractive index [12] using a microscope that combines AFM with laser confocal microscopy. In addition, we investigated in-situ observation of stratum corneum in a glycerin solution by laser confocal interference microscopy and the dynamics of the SC in a glycerin solution [13].…”

Section: Introductionmentioning

confidence: 99%

Mechanical Properties of Stratum Corneum in Glycerin Solution by Atomic Force Microscopy

Yanagiya

Takahashi

Goto

2015

e-J. Surf. Sci. Nanotech.

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Stratum corneum (SC), the outermost skin layer, consists of corneocytes and intercellular lipids. In this study, mechanical properties of a cultured human SC and a tape-stripped human corneocyte were examined in a glycerine solution through atomic force microscopy. A multilayered cultured human SC in the low concentration glycerin solution decreased its Young's modulus with time. Besides the corneocyte increased its adhesion force in higher concentration of glycerin solution.

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“…In our previous study, we observed interference patterns on an AFM cantilever. We then reported the misalignment of fringes corresponding to the step heights of a potassium dihydrogen phosphate crystal by using LCM 5 and a relative refractive index of a small volume of water. 6 In the present study, we chose a glycerin solution as the aqueous solution.…”

mentioning

confidence: 99%

In Situ Observation of Stratum Corneum Using Cantilever-assisted Two-beam Interference Microscopy

Yanagiya¹,

Katayama²,

Goto³

2012

Chemistry Letters

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We demonstrated in situ observation of the swelling of stratum corneum (SC) by cantilever-assisted two-beam interference microscopy (CATBIM) in this study. The CATBIM enabled us to observe the refractive index changes of the small region of a stratum corneum in 1% glycerin solution. Interference pattern images of the SC were successfully acquired. The fringe interval was almost the same during the experiments. On the other hand, the thickness by swelling of the SC increased with time. The speeds of swelling decreased with time.Skin covers the human body and has functions such as protection, sensation, and controlling the evaporation. The skin consists of several layers and stratum corneum (SC), which is the outermost part of the skin. The SC consists of corneocytes and intercellular lipids. It directly protects the internal body from the environment and absorbs medicines or cosmetics that we apply to prevent evaporation and permeation into the skin cells. The aim of this study was to clarify the mechanism of osmosis at the cell and tissue level.In recent years, there have been studies of the skin at the cell or tissues level.13 These have studied mostly with atomic force microscopy (AFM) and nanoindentor. Yuan and Verma reported microelastic properties of wet and dry SC.2 Bhushan has reported the nanotribological and nanomechanical properties of the skin and the effects of skin cream.1 Crichton et al. measured the viscoelastic property of each layer of the human skin.3 Laser confocal microscopy (LCM) has also been used in cell or tissue biology. Vergou et al. reported the comparison between transepidermal water loss and LCM measurements. 4 Most experiments were performed in air because it is natural. Conversely, this study focused on the dynamics of the SC in an aqueous solution.We have studied soft materials by using a combined microscope that consists of AFM and LCM. In our previous study, we observed interference patterns on an AFM cantilever. We then reported the misalignment of fringes corresponding to the step heights of a potassium dihydrogen phosphate crystal by using LCM 5 and a relative refractive index of a small volume of water. 6 In the present study, we chose a glycerin solution as the aqueous solution. Glycerin solution is an aqueous alcohol and is widely used as a food and pharmaceutical additive. The glycerin shows high viscosity and decreases the coefficient of friction of surfaces. 1,7 In the present study, we investigated in situ observation of stratum corneum in a glycerin solution by interference microscopy, which is produced by the combined setup of LCM and AFM. And the swelling dynamics of the SC in a glycerin solution was also studied.First, we describe the theoretical interference model of this study. Interference phenomena, which were observed on an AFM cantilever bar using an optical microscope, were first reported by Owen et al. 8 As previously reported, 5,6 the behavior of the interference fringes informs us of the refractive index of media or the geometric lengths of the light path...

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Hybrid observation of crystal growth using laser confocal microscope with atomic force microscope

Cited by 10 publications

References 12 publications

Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal

Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal

Mechanical Properties of Stratum Corneum in Glycerin Solution by Atomic Force Microscopy

In Situ Observation of Stratum Corneum Using Cantilever-assisted Two-beam Interference Microscopy

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