Hybrid nanoparticles with cell membrane and dexamethasone-conjugated polymer for gene delivery into the lungs as therapy for acute lung injury

Zhuang, Chuanyu; Piao, Chunxian; Kang, Minji; Oh, Jihun; Lee, Minhyung

doi:10.1039/d2bm02109a

Cited by 8 publications

(5 citation statements)

References 33 publications

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“…Zhuang et al prepared a delivery system coated with LA-4 lung epithelial cell membrane (CM) that significantly improved the delivery efficiency of pDNA to the lungs, surpassing the anti-inflammatory effect of the delivery system without cell membrane encapsulation. 125…”

Section: Strategies To Improve Gene Transfection Efficiencymentioning

confidence: 99%

“…Zhuang et al developed a hybrid nanoparticle combining dexamethasone-coupled polyethyleneimine (DP) with cell membranes (CM) from LA-4 lung epithelial cells. 125 The aim was to improve the delivery efficiency of pDNA to the lungs. The CM in the hybrid nanoparticles interacted with the plasma membrane of the target cells, promoting cellular uptake of pDNA.…”

Section: Biological Applicationmentioning

confidence: 99%

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Recent developments of polymeric delivery systems in gene therapeutics

Li,

Tian,

et al. 2024

Polym. Chem.

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Section: Strategies To Improve Gene Transfection Efficiencymentioning

confidence: 99%

Section: Biological Applicationmentioning

confidence: 99%

Recent developments of polymeric delivery systems in gene therapeutics

Li,

Tian,

et al. 2024

Polym. Chem.

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“…The use of cell membranes or microvesicles to encapsulate nanoparticles reduces their recognition and removal by the immune system, as these membranes are derived from the body’s own cells and are naturally biocompatible. Cell membranes or microvesicles may contain specific proteins or receptors that can bind to specific markers in lung cells, thus improving lung targeting [ 156 ]. Camouflaged nanoparticles can cross biological barriers, such as the blood‒air barrier, more efficiently, thereby increasing the efficiency of the drug in reaching the lungs.…”

Section: Types Of Nanomaterials Targeted To Treat Alimentioning

confidence: 99%

Advances in nanomaterial-targeted treatment of acute lung injury after burns

Zhang,

Zhao,

Xue

et al. 2024

J Nanobiotechnol

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Acute lung injury (ALI) is a common complication in patients with severe burns and has a complex pathogenesis and high morbidity and mortality rates. A variety of drugs have been identified in the clinic for the treatment of ALI, but they have toxic side effects caused by easy degradation in the body and distribution throughout the body. In recent years, as the understanding of the mechanism underlying ALI has improved, scholars have developed a variety of new nanomaterials that can be safely and effectively targeted for the treatment of ALI. Most of these methods involve nanomaterials such as lipids, organic polymers, peptides, extracellular vesicles or cell membranes, inorganic nanoparticles and other nanomaterials, which are targeted to reach lung tissues to perform their functions through active targeting or passive targeting, a process that involves a variety of cells or organelles. In this review, first, the mechanisms and pathophysiological features of ALI occurrence after burn injury are reviewed, potential therapeutic targets for ALI are summarized, existing nanomaterials for the targeted treatment of ALI are classified, and possible problems and challenges of nanomaterials in the targeted treatment of ALI are discussed to provide a reference for the development of nanomaterials for the targeted treatment of ALI.

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“…9−11 Besides, the LPEImediated intravenous gene delivery affords the highest level of transgene expression selectively in the lung tissue. 12,13 This targeted expression in the lung implies massive potential of the LPEI and its analogues for nonviral gene therapy against lung cancer. For example, Rodrigo-Garzoń et al showed intravenous IL-12 gene transfer by the LPEI for the gene therapy against metastatic lung cancer.…”

Section: Introductionmentioning

confidence: 99%

“…As an alternative to those BPEIs, 25 kDa linear PEI (LPEI) holds much lower in vitro and in vivo toxicity as compared to the BPEI, thus enabling solid tumor gene therapy by intravenous or intratumoral injection. − Besides, the LPEI-mediated intravenous gene delivery affords the highest level of transgene expression selectively in the lung tissue. , This targeted expression in the lung implies massive potential of the LPEI and its analogues for nonviral gene therapy against lung cancer. For example, Rodrigo-Garzón et al showed intravenous IL-12 gene transfer by the LPEI for the gene therapy against metastatic lung cancer .…”

Section: Introductionmentioning

confidence: 99%

Degradable Cationic Polycarbamates Designed for Robust IL-12 Gene Therapy against Metastatic Lung Cancer

An,

Li,

et al. 2024

ACS Appl. Polym. Mater.

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Polyethylenimine (PEI)-based gene carriers hold a massive promise for nonviral gene delivery against cancers. However, their poor degradability regularly results in rather high cytotoxicity, thus seriously hampering further clinical translations. Herein, a group of degradable cationic polycarbamates (CPCs) were finely designed to possess similar chemical structures with the PEIs for nonviral gene transfection. These CPCs containing multiple primary (or secondary) plus tertiary amino groups were synthesized by the polycondensation reaction of tertiary amino-containing primary diamines and the biscarbonates with butoxycarbonyl-protected primary (or secondary) amino groups, followed by the removal of the protecting group. These CPCs displayed degradation profiles under physiological conditions and degraded more rapidly when comprising the secondary amino groups compared with the primary amino residues. Moreover, these CPCs had favorable gene delivery properties including strong gene condensation ability and high buffering capacity as well as less cytotoxicity in comparison with nondegradable PEI controls, giving rise to efficient in vitro gene transfection in varied cells. Particularly, the secondary amino-containing CPCs based on the Nmethyl-2, 2′-diaminodiethylamine (denoted as BHE-MDE) caused excellent in vitro transfection activity selectively in type-II alveolar epithelial cells compared to other cells. The transfection efficiency of the BHE-MDE in the epithelial cells was comparable to that of 25 kDa linear PEI (LPEI) as a positive control. In the C57BL/6 mouse model, similar to this positive control, the BHE-MDE induced a high level of transgene expression in the lung and marked IL-12 expression in the mouse peripheral blood. As such, the BHE-MDE-mediated IL-12 expression afforded highly robust gene therapy against metastatic LLC cells in the lung of mice, thereby prolonging their survival time. The results indicate great potential of degradable LPEI-mimetic CPCs for nonviral gene therapy against metastatic lung cancer.

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Hybrid nanoparticles with cell membrane and dexamethasone-conjugated polymer for gene delivery into the lungs as therapy for acute lung injury

Abstract: Gene therapy has been suggested as a new treatment for acute lung injury (ALI), which is a severe inflammatory disease. Previously, amphiphilic polymeric carriers such as dexamethasone-conjugated polyethylenimine (PEI) (DP)...

Cited by 8 publications

References 33 publications

Recent developments of polymeric delivery systems in gene therapeutics

Recent developments of polymeric delivery systems in gene therapeutics

Advances in nanomaterial-targeted treatment of acute lung injury after burns

Degradable Cationic Polycarbamates Designed for Robust IL-12 Gene Therapy against Metastatic Lung Cancer

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