Hybrid multimode - multicore fibre based holographic endoscope for deep-tissue neurophotonics

Du, Yu; Turtaev, Sergey; Leite, Ivo T.; Lorenz, Adrian; Kobelke, Jens; Wondraczek, Katrin; Čižmár, Tomáš

doi:10.37188/lam.2022.029

Cited by 11 publications

(8 citation statements)

References 34 publications

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“…2(a) ] consists of 61 corelets, as reported previously. 34 The overall cladding and pulp, with diameters of

and

, respectively, form a step-index multimode waveguide with a refractive index difference corresponding to an NA of 0.1. In the multimode regime, as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The TM is acquired using phase-shift interferometry and is used to generate diffraction-limited foci at the

output, as described in detail in our previous work. 34 After the TM acquisition, the calibration module at the fiber distal end is removed and replaced by the mouse.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous work, 34 we presented a novel hybrid multimode-multicore fiber (

) with 61 corelets. This fiber features a cascaded refractive index structure and allows for two distinct regimes of light transport that mimic either a multimode or a multicore fiber.…”

Section: Introductionmentioning

confidence: 99%

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Advancing the path to in-vivo imaging in freely moving mice via multimode-multicore fiber based holographic endoscopy

Du,

Dylda,

Stibůrek

et al. 2024

Neurophoton.

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. Significance Hair-thin multimode optical fiber-based holographic endoscopes have gained considerable interest in modern neuroscience for their ability to achieve cellular and even subcellular resolution during in-vivo deep brain imaging. However, the application of multimode fibers in freely moving animals presents a persistent challenge as it is difficult to maintain optimal imaging performance while the fiber undergoes deformations. Aim We propose a fiber solution for challenging in-vivo applications with the capability of deep brain high spatial resolution imaging and neuronal activity monitoring in anesthetized as well as awake behaving mice. Approach We used our previously developed multimode-multicore fiber to record fluorescently labeled neurons in anesthetized mice. Our exhibits a cascaded refractive index structure, enabling two distinct regimes of light transport that imitate either a multimode or a multicore fiber. The has been specifically designed for use in the initial phase of an in-vivo experiment, allowing for the navigation of the endoscope’s distal end toward the targeted brain structure. The multicore regime enables the transfer of light to and from each individual neuron within the field of view. For chronic experiments in awake behaving mice, it is crucial to allow for disconnecting the fiber and the animal between experiments. Therefore, we provide here an effective solution and establish a protocol for reconnection of two segments of with hexagonally arranged corelets. Results We successfully utilized the to image neurons in anaesthetized transgenic mice expressing enhanced green fluorescent protein. Additionally, we compared imaging results obtained with the with larger numerical aperture (NA) fibers in fixed whole-brain tissue. Conclusions This study focuses on addressing challenges and providing insights into the use of multimode-multicore fibers as imaging solutions for in-vivo applications. We suggest that the upcoming version of the increases the overall NA between the two cladding layers to allow for access to high resolution spatial imaging. As the NA increases in the multimode regime, the fiber diameter and ring structure must be reduced to minimize the computational burden and invasiveness.

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“…2(a) ] consists of 61 corelets, as reported previously. 34 The overall cladding and pulp, with diameters of

and

, respectively, form a step-index multimode waveguide with a refractive index difference corresponding to an NA of 0.1. In the multimode regime, as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The TM is acquired using phase-shift interferometry and is used to generate diffraction-limited foci at the

output, as described in detail in our previous work. 34 After the TM acquisition, the calibration module at the fiber distal end is removed and replaced by the mouse.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Advancing the path to in-vivo imaging in freely moving mice via multimode-multicore fiber based holographic endoscopy

Du,

Dylda,

Stibůrek

et al. 2024

Neurophoton.

Self Cite

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show abstract

“…The power of this strategy relies on the possibility to regenerate the object by illuminating the hologram with the reference wave. This property determined an outstanding interest from the scientific community spanning biotechnology, [147] neuroscience, [148,149] fiber-optics technology, [150] metasurfaces, [151,152] and data storage, [153] just to mention a few.…”

Section: General Aspects and Theoretical Overviewmentioning

confidence: 99%

Recent Advances on High‐Speed and Holographic Two‐Photon Direct Laser Writing

Balena

Bianco

Pisanello

et al. 2023

Adv Funct Materials

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Two‐Photon Lithography, thanks to its very high sub‐diffraction resolution, has become the lithographic technique par excellence in applications requiring small feature sizes and complex 3D pattering. Despite this, the fabrication times required for extended structures remain much longer than those of other competing techniques (UV mask lithography, nanoimprinting, etc.). Its low throughput prevents its wide adoption in industrial applications. To increase it, over the years different solutions have been proposed, although their usage is difficult to generalize and may be limited depending on the specific application. A promising strategy to further increase the throughput of Two‐Photon Lithography, opening a concrete window for its adoption in industry, lies in its combination with holography approaches: in this way it is possible to generate dozens of foci from a single laser beam, thus parallelizing the fabrication of periodic structures, or to engineer the intensity distribution on the writing plane in a complex way, obtaining 3D microstructures with a single exposure. Here, the fundamental concepts behind high‐speed Two‐Photon Lithography and its combination with holography are discussed, and the literary production of recent years that exploits such techniques is reviewed, and contextualized according to the topic covered.

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“…[85][86][87][88] Rather than repeatedly recalibrating the transmission matrix, there also have been continuous efforts to design a bending-invariant structure of optical fibers, 89 such as an MMF with a parabolic refractive index profile, 90 a twisted MCF, 91 and even a hybrid structure of the MMF and MCF for both high-resolution imaging and motion-insensitive low-resolution image. 92 Alternatively, to circumvent the calibration issue in using an MMF, some approaches have been proposed to encode the spatial information to the spectral information by placing a spatiospectral encoder at the distal tip of a fiber 93,94 and reconstruct an image from spectrally resolved detection at the proximal tip.…”

Section: In Situ Transmission Matrix Calibrationmentioning

confidence: 99%

Review of endomicroscopic imaging with coherent manipulation of light through an ultrathin probe

Lee

Song

et al. 2023

J. Optical Microsystems

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Endomicroscopy is a technique to visualize microscopic structures of internal tissues through tubular instruments that can be inserted through a small cut or an opening in the body. There has been a growing demand for miniaturizing endoscopic instruments while preserving a high resolution to achieve a real-time histopathologic diagnosis. Meanwhile, there has recently been tremendous progress in the coherent manipulation of light in which an optical wave is deterministically manipulated through a linear, disordered medium for optical focusing and imaging. Here, we review recent research efforts in developing new endomicroscopic imaging schemes based on the coherent formulation and manipulation of optical fibers. In contrast to the conventional schemes using optical fibers as incoherent channels for optical power, these approaches provide a route to fully exploiting useful information transmitted through an ultrathin probe, thereby potentially achieving practical endomicroscopic imaging through a submillimeter thick probe.

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Hybrid multimode - multicore fibre based holographic endoscope for deep-tissue neurophotonics

Cited by 11 publications

References 34 publications

Advancing the path to in-vivo imaging in freely moving mice via multimode-multicore fiber based holographic endoscopy

Advancing the path to in-vivo imaging in freely moving mice via multimode-multicore fiber based holographic endoscopy

Recent Advances on High‐Speed and Holographic Two‐Photon Direct Laser Writing

Review of endomicroscopic imaging with coherent manipulation of light through an ultrathin probe

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