Hybrid M-mode-like OCT imaging of three-dimensional microvasculature in vivo using reference-free processing of complex valued B-scans

Matveev, Lev A.; Zaitsev, Vladimir Yu.; Gelikonov, Grigory V.; Matveyev, Alexander L.; Moiseev, Alexander A.; Ksenofontov, S. Yu.; Gelikonov, Valentin M.; Sirotkina, Marina A.; Gladkova, Natalia D.; Demidov, Valentin; Vitkin, I. Alex

doi:10.1364/ol.40.001472

Cited by 62 publications

(61 citation statements)

References 27 publications

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“…17,18 In addition to the high resolution svOCT imaging of microvasculature, it has potential to provide new insight into the dynamics of vascular remodeling. [19][20][21] In our previous phantom study, 11 we demonstrated that MBs can be used as an optical contrast agent, yielding increased scattering (1.4× at 300 nm and 6× at 850 nm). Similarly, Barton et al 10 have shown that MBs enhanced the backscattered OCT signal in model scattering media.…”

Section: Introductionmentioning

confidence: 86%

Microvascular contrast enhancement in optical coherence tomography using microbubbles

et al. 2016

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Gas microbubbles (MBs) are investigated as intravascular optical coherence tomography (OCT) contrast agents. Agar + intralipid scattering tissue phantoms with two embedded microtubes were fabricated to model vascular blood flow. One was filled with human blood, and the other with a mixture of human blood + MB. Swept-source structural and speckle variance (sv) OCT images, as well as speckle decorrelation times, were evaluated under both no-flow and varying flow conditions. Faster decorrelation times and higher structural and svOCT image contrasts were detected in the presence of MB in all experiments. The effects were largest in the svOCT imaging mode, and uniformly diminished with increasing flow velocity. These findings suggest the feasibility of utilizing MB for tissue hemodynamic investigations and for microvasculature contrast enhancement in OCT angiography.

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Section: Introductionmentioning

confidence: 86%

Microvascular contrast enhancement in optical coherence tomography using microbubbles

et al. 2016

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“…This can be accomplished, for example, using conventional procedures of least-squares fitting 24 of the depth-dependent phase-variation within a selected size of the processing window. Before fitting the slope of the axial (vertical) dependence of the phase variation, for every horizontal row of pixels within the processing window, it is useful to determine the average phase variation at that depth, for example, using the Kasai estimator 33,34 or a somewhat different but analogous procedure described below in this section. The least-square procedure 24 of fitting the vertical phase-variation to determine the phase-variation gradient [ Fig.…”

Section: Comparison Of Phase-gradient Estimation Methods For Finding mentioning

confidence: 99%

Optimized phase gradient measurements and phase-amplitude interplay in optical coherence elastography

et al. 2016

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In compressional optical coherence elastography, phase-variation gradients are used for estimating quasistatic strains created in tissue. Using reference and deformed optical coherence tomography (OCT) scans, one typically compares phases from pixels with the same coordinates in both scans. Usually, this limits the allowable strains to fairly small values <10?4 to 10?3, with the caveat that such weak phase gradients may become corrupted by stronger measurement noises. Here, we extend the OCT phase-resolved elastographic methodology by (1) showing that an order of magnitude greater strains can significantly increase the accuracy of derived phase-gradient differences, while also avoiding error-phone phase-unwrapping procedures and minimizing the influence of decorrelation noise caused by suprapixel displacements, (2) discussing the appearance of artifactual stiff inclusions in resultant OCT elastograms in the vicinity of bright scatterers due to the amplitude-phase interplay in phase-variation measurements, and (3) deriving/evaluating methods of phase-gradient estimation that can outperform conventionally used least-square gradient fitting. We present analytical arguments, numerical simulations, and experimental examples to demonstrate the advantages of the proposed optimized phase-variation methodology.

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“…OCT angiography concept implemented in the device is based on the analysis of decorrelation of OCT image speckle pattern induced by blood flow within tissue. 36,37 The device is equipped with the contact probe, and the probe pressure to the tissue was controlled by a custom-designed holder to avoid effects of tissue compression on OCT images. 38 OCT imaging of the target area was performed prior to and immediately after the exposure and in 1, 4, and 7 days after the procedure (in 24, 96, and 168 hours, respectively).…”

Section: Optical Diagnostics Modalitiesmentioning

confidence: 99%

Comparative analysis of single- and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study

Kurakina¹,

Khilov²,

Shakhova

et al. 2019

J. Biomed. Opt.

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Comparative analysis of single-and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study," J.Abstract. Two pronounced absorption peaks in blue and red ranges of the chlorin-based photosensitizer (PS) absorption spectrum provide additional benefits in photodynamic therapy (PDT) performance. Differing optical properties of biological tissues in these ranges allow for both dual-wavelength diagnostics and PDT performance. We provide a comparative analysis of different PDT regimes performed with blue and red lights and their combination, with doses varying from 50 to 150 J∕cm 2 . The study was performed on the intact skin of a rabbit ear inner surface, with the use of chlorin e6 as a PS. PDT procedure protocol included monitoring of the treated site with fluorescence imaging technique to evaluate PS accumulation and photobleaching, as well as with optical coherence tomography (OCT) to register morphological and functional responses of the tissue. Optical diagnostic observations were compared with the results of histopathology examination. We demonstrated that PDT procedures with the considered regimes induce weaker organism reaction manifested by edema in normal tissue as compared to irradiation-only exposures with the same light doses. The light doses delivered with red light induce weaker tissue reaction as compared to the same doses delivered with blue light only or with a combination of red and blue lights in equal parts. Results of in-vivo OCT monitoring of tissue reaction are in agreement with the results of histopathology study.

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Hybrid M-mode-like OCT imaging of three-dimensional microvasculature in vivo using reference-free processing of complex valued B-scans

Cited by 62 publications

References 27 publications

Microvascular contrast enhancement in optical coherence tomography using microbubbles

Microvascular contrast enhancement in optical coherence tomography using microbubbles

Optimized phase gradient measurements and phase-amplitude interplay in optical coherence elastography

Comparative analysis of single- and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study

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