Hybrid laser technology and doped biomaterials

Jelı́nek, M.; Zemek, J.; Remsa, Jan; Mikšovský, Jan; Kocourek, T.; Písařík, Petr; Trávníčková, Martina; Filová, Elena; Bačáková, Lucie

doi:10.1016/j.apsusc.2017.03.103

Cited by 17 publications

(31 citation statements)

References 15 publications

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“…DLC layers doped with Cr were prepared by a hybrid laser technology combining simultaneous pulsedlaser deposition (PLD) and magnetron sputtering on fused silica substrates, at room temperature. A precise description of this process has already been published (Jelinek et al, 2015;Jelinek et al, 2017;Jelinek et al, 2016b). Briefly, for the deposition, a KrF excimer laser was used for carbon deposition, while a magnetron (2-inch, K. J. Lesker) sputtered chromium species (~ 5 eV).…”

Section: Materials and Methods Preparation And Characterisation Of Crmentioning

confidence: 99%

“…In an earlier study it was shown that, under a critical mechanical load, the delaminated pieces of the layers were larger in Ti-doped DLC than in Cr-doped DLC. In addition, Cr-doped DLC films were harder than Ti-doped DLC films (Jelinek et al, 2017). Modifying DLC with Cr could therefore be advantageous for coating biomedical implants; however, the biological effect of the Cr added in the DLC layer needs to be elucidated.…”

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confidence: 99%

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Effect of diamond-like carbon doped with chromium on cell differentiation, immune activation and apoptosis

Trávníčková¹,

Vandrovcová²,

Filová

et al. 2020

eCM

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Diamond-like carbon (DLC) is a biocompatible material that has many potential biomedical applications, including in orthopaedics. DLC layers doped with Cr at atomic percent (at.%) of 0, 0.9, 1.8, 7.3, and 7.7 at.% were evaluated with reference to their osteoinductivity with human bone marrow mesenchymal stromal cells (hMSCs), immune activation potential with RAW 264.7 macrophage-like cells, and their effect on apoptosis in Saos-2 human osteoblast-like cells and neonatal human dermal fibroblasts (NHDFs). At mRNA level, hMSCs on DLC doped with 0.9 and 7.7 at.% of Cr reached higher maximum values of both RUNX2 and alkaline phosphatase. An earlier onset of mRNA production of type I collagen and osteocalcin was also observed on these samples; they also supported the production of both type I collagen and osteocalcin. RAW 264.7 macrophages were screened using a RayBio™ Human Cytokine Array for cytokine production. 10 cytokines were at a concentration more than 2 × as high as the concentration of a positive control, but the values for the DLC samples were only moderately higher than the values on glass. NHDF cells, but not Saos-2 cells, had a higher expression of pro-apoptotic markers Bax and Bim and a lower expression of anti-apoptotic factor BCL-XL in proportion to the Cr content. Increased apoptosis was also proven by annexin V staining. These results show that a Cr-doped DLC layer with a lower Cr content can act as an osteoinductive material with relatively low immunogenicity, but that a higher Cr content can induce cell apoptosis.

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Section: Materials and Methods Preparation And Characterisation Of Crmentioning

confidence: 99%

mentioning

confidence: 99%

Effect of diamond-like carbon doped with chromium on cell differentiation, immune activation and apoptosis

Trávníčková¹,

Vandrovcová²,

Filová

et al. 2020

eCM

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“…Pulsed-laser technology can also be applied for depositing Cr-doped or Ti-doped diamond-like carbon films on titanium implants. These films also have promising osseointegration properties 94 .…”

Section: Laser Treatmentmentioning

confidence: 99%

Treatments for enhancing the biocompatibility of titanium implants

Štěpanovská¹,

Matějka²,

Rosina³

et al. 2020

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Titanium surface treatment is a crucial process for achieving sufficient osseointegration of an implant into the bone. If the implant does not heal sufficiently, serious complications may occur, e.g. infection, inflammation, aseptic loosening of the implant, or the stress-shielding effect, as a result of which the implant may need to be reoperated. After a titanium graft has been implanted, several interactions are crucial in order to create a strong bone-implant connection. It is essential that cells adhere to the surface of the implant. Surface roughness has a significant influence on cell adhesion, and also on improving and accelerating osseointegration. Other highly important factors are biocompatibility and resistance to bacterial contamination. Bio-inertness of titanium is ensured by the protective film of titanium oxides that forms spontaneously on its surface. This film prevents the penetration of metal compounds, and it is well-adhesive for calcium and phosphate ions, which are necessary for the formation of the mineralized bone structure. Since the presence of the film alone is not sufficient for the biocompatibility of titanium, a suitable surface finish is required to create a firm bone-implant connection. In this review, we explain and compare the most widely-used methods for modulating the surface roughness of titanium implants in order to enhance cell adhesion on the surface of the implant, e.g. plasma spraying, sandblasting, acid etching, laser treatment, sol-gel etc., The methods are divided into three overlapping groups, according to the type of modification. ) is gratefully acknowledged for his language revision of the manuscript. We also thank the Prospon s.r.o. company (Kladno, Czech Republic) for providing Ti6Al4V samples for studies on cell-material interaction.

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“…Therefore, metal nanoparticles are embedded into the a-C:H matrix. Modifying DLC with different metallic elements such as Cr [42,43], Ti [42,44], W [45,46] and Mo [46] can lead to new functions of the DLC thin films. Adding non-carbide-forming elements like Ag [47,48], Cu [49], Al [50] and Ni [50] into the DLC matrix can reduce residual stress [43,45,[47][48][49][50] and improve electric conductivity [44,51].…”

Section: Introductionmentioning

confidence: 99%

Surface Characterization and Copper Release of a-C:H:Cu Coatings for Medical Applications

et al. 2019

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This paper focuses on the surface properties of a-C:H:Cu composite coatings for medical devices and how the release of Cu2+ ions from such coatings can be controlled. The released Cu ions have the potential to act as a bactericidal agent and inhibit bacterial colonization. A PVD–PECVD hybrid process was used to deposit a-C:H:Cu composite coatings onto Ti6Al4V substrates. We examine the layer surface properties using atomic force microscopy and static contact angle measurements. An increasing surface roughness and increasing contact angle of Ringer’s solution was measured with increasing copper mole fraction (XCu) in the coatings. The contact angle decreased when a supplementary bias voltage of −50 V was used during the a-C:H:Cu deposition. These findings are in line with earlier published results regarding these types of coatings. The release of Cu2+ ions from a-C:H:Cu coatings in Ringer’s solution was measured by anodic stripping voltammetry. Different layer structures were examined to control the time-resolved Cu release. It was found that the Cu release depends on the overall XCu in the a-C:H:Cu coatings and that an additional a-C:H barrier layer on top of the a-C:H:Cu layer effectively delays the release of Cu ions.

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Hybrid laser technology and doped biomaterials

Cited by 17 publications

References 15 publications

Effect of diamond-like carbon doped with chromium on cell differentiation, immune activation and apoptosis

Effect of diamond-like carbon doped with chromium on cell differentiation, immune activation and apoptosis

Treatments for enhancing the biocompatibility of titanium implants

Surface Characterization and Copper Release of a-C:H:Cu Coatings for Medical Applications

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