Hybrid inorganic-organic fluorescent silica nanoparticles—influence of dye binding modes on dye leaching

Klippel, Nadja; Jung, Gregor; Kickelbick, Guido

doi:10.1007/s10971-021-05578-y

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…The initially pursued attempt to also use NR as a reference dye for the SiO 2 -NPs failed because the incorporation of NR into the SiO 2 matrix by adsorptive interactions led to an increase in particle size (SI, Fig. S21 ), and the dye molecules were washed out during NP purification (data not shown), as has been reported before for other dyes 62 . The synthesis of a NR silane derivative (SI, Fig.…”

Section: Resultsmentioning

confidence: 77%

Dual color pH probes made from silica and polystyrene nanoparticles and their performance in cell studies

Srivastava

Tavernaro

Scholtz

et al. 2023

Sci Rep

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Ratiometric green–red fluorescent nanosensors for fluorometrically monitoring pH in the acidic range were designed from 80 nm-sized polystyrene (PS) and silica (SiO2) nanoparticles (NPs), red emissive reference dyes, and a green emissive naphthalimide pH probe, analytically and spectroscopically characterized, and compared regarding their sensing performance in aqueous dispersion and in cellular uptake studies. Preparation of these optical probes, which are excitable by 405 nm laser or LED light sources, involved the encapsulation of the pH-inert red-fluorescent dye Nile Red (NR) in the core of self-made carboxylated PSNPs by a simple swelling procedure and the fabrication of rhodamine B (RhB)-stained SiO2-NPs from a silane derivative of pH-insensitive RhB. Subsequently, the custom-made naphthalimide pH probe, that utilizes a protonation-controlled photoinduced electron transfer process, was covalently attached to the carboxylic acid groups at the surface of both types of NPs. Fluorescence microscopy studies with the molecular and nanoscale optical probes and A549 lung cancer cells confirmed the cellular uptake of all probes and their penetration into acidic cell compartments, i.e., the lysosomes, indicated by the switching ON of the green naphthalimide fluorescence. This underlines their suitability for intracellular pH sensing, with the SiO2-based nanosensor revealing the best performance regarding uptake speed and stability.

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Section: Resultsmentioning

confidence: 77%

Dual color pH probes made from silica and polystyrene nanoparticles and their performance in cell studies

Srivastava

Tavernaro

Scholtz

et al. 2023

Sci Rep

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“…The primary amino groups on the surface of pHOP-NH 2 particles were covalently coupled with CSN-functional groups of FITC dye via thiourea bonds. This covalent incorporation of FITC onto the pHOPs could minimize the FITC dye leaching [ 20 , 21 ]. The size and morphology of synthesized F-HOPs were characterized by TEM.…”

Section: Resultsmentioning

confidence: 99%

Amino Surface Modification and Fluorescent Labelling of Porous Hollow Organosilica Particles: Optimization and Characterization

et al. 2022

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Surface modification of silica nanoparticles with organic functional groups while maintaining colloidal stability remains a synthetic challenge. This work aimed to prepare highly dispersed porous hollow organosilica particles (pHOPs) with amino surface modification. The amino-surface modification of pHOPs was carried out with 3-aminopropyl(diethoxy)methylsilane (APDEMS) under various reaction parameters, and the optimal pHOP-NH2 sample was selected and labelled with fluorescein isothiocyanate (FITC) to achieve fluorescent pHOPs (F-HOPs). The prepared pHOPs were thoroughly characterized by transmission electron microscopy, dynamic light scattering, FT-IR, UV-Vis and fluorescence spectroscopies, and microfluidic resistive pulse sensing. The optimal amino surface modification of pHOPs with APDEMS was at pH 10.2, at 60 °C temperature with 10 min reaction time. The positive Zeta potential of pHOP-NH2 in an acidic environment and the appearance of vibrations characteristic to the surface amino groups on the FT-IR spectra prove the successful surface modification. A red-shift in the absorbance spectrum and the appearance of bands characteristic to secondary amines in the FTIR spectrum of F-HOP confirmed the covalent attachment of FITC to pHOP-NH2. This study provides a step-by-step synthetic optimization and characterization of fluorescently labelled organosilica particles to enhance their optical properties and extend their applications.

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“…Encapsulating FITC molecules within the SiO 2 matrix offers enhanced and sustained fluorescence 7 . This encapsulation method also stabilizes FITC against pH-related variations and shields it from photobleaching 13 , 14 .…”

Section: Introductionmentioning

confidence: 99%

“…Various methodologies have been developed to produce FITC-SiO 2 NPs 14 , 18 . A prevalent approach involves trapping the dyes within the particles, usually initiated by a covalent pre-conjugation of the dye to a suitable functional group.…”

Section: Introductionmentioning

confidence: 99%

High biocompatible FITC-conjugated silica nanoparticles for cell labeling in both in vitro and in vivo models

Nguyen,

Nghiem

et al. 2024

Sci Rep

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Fluorescence nanosilica-based cell tracker has been explored and applied in cell biological research. However, the aggregation of these nanoparticles at physiological pH is still the main limitation. In this research, we introduced a novel fluorescence nano-based cell tracker suitable for application in live cells. The silica-coated fluorescein isothiocyanate isomer (FITC-SiO2) nanoparticles (NPs) were modified with carboxymethylsilanetriol disodium salt (FITC-SiO2-COOH), integrating the dianion form of FITC molecules. This nanosystem exhibited superior dispersion in aqueous solutions and effectively mitigated dye leakage. These labeled NPs displayed notable biocompatibility and minimal cytotoxicity in both in vitro and in vivo conditions. Significantly, the NPs did not have negative implications on cell migration or angiogenesis. They successfully penetrated primary fibroblasts, human umbilical vein endothelial cells and HeLa cells in both 2D and 3D cultures, with the fluorescence signal enduring for over 72 h. Furthermore, the NP signals were consistently observed in the developing gastrointestinal tract of live medaka fish larvae for extended periods during phases of subdued digestive activity, without manifesting any apparent acute toxicity. These results underscore the promising utility of FITC-SiO2-COOH NPs as advanced live cell trackers in biological research.

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Hybrid inorganic-organic fluorescent silica nanoparticles—influence of dye binding modes on dye leaching

Cited by 9 publications

References 52 publications

Dual color pH probes made from silica and polystyrene nanoparticles and their performance in cell studies

Dual color pH probes made from silica and polystyrene nanoparticles and their performance in cell studies

Amino Surface Modification and Fluorescent Labelling of Porous Hollow Organosilica Particles: Optimization and Characterization

High biocompatible FITC-conjugated silica nanoparticles for cell labeling in both in vitro and in vivo models

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