Hybrid genes over-express pertactin from Bordetella pertussis

Loosmore, Sheena M.; Yacoob, Reza K.; Zealey, Gavin; Jackson, G; Yang, Yanping; Chong, Pele; Shortreed, Jean M.; Coleman, Debbie C.; Cunningham, J. D.; Gisonni, Lucy; Klein, Michel

doi:10.1016/0264-410x(94)00015-f

Cited by 14 publications

(9 citation statements)

References 50 publications

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“…4b). In this study, both CD and fluorescence spectra obtained were similar to those reported previously [27,28], which can be seen as evidence for the correct senior structure maintained through purification process.…”

Section: Analysis Of Purified Pertactinsupporting

confidence: 88%

“…This typical ellipticity together with no obvious negative minimum ellipticity at 222 nm represents a dominant proportion of b-sheet and no apparent a-helix [21]. The shape of far-UV CD spectrum of the obtained protein was similar to that of pertactin in the culture supernatant [27], suggesting that the heat release for protein extraction could have little influence on the three dimensional structure of pertactin. Secondary structure analysis based on the spectrum estimated a composition of 12.5% a-helix, 55.6% b-sheet, 7.9% b-turn and 24% random coil with include software, which is in accordance with the crystal structure (PDB: 1DAB).…”

Section: Analysis Of Purified Pertactinmentioning

confidence: 90%

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Purification design and practice for pertactin, the third component of acellular pertussis vaccine, from Bordetella pertussis

Zhang

et al. 2016

Vaccine

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Section: Analysis Of Purified Pertactinsupporting

confidence: 88%

Section: Analysis Of Purified Pertactinmentioning

confidence: 90%

Purification design and practice for pertactin, the third component of acellular pertussis vaccine, from Bordetella pertussis

Zhang

et al. 2016

Vaccine

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“…After the PBMCs were washed, cells were cultured in AIMV medium (Gibco Invitrogen, Grand Island, NY) containing 5% human AB serum (Harlan Laboratories, Leicestershire, United Kingdom). The cells were stimulated with 5 g/ml PT or 10 g/ml FHA (Novartis, Siena, Italy) or 4 g/ml recombinant Prn (20) at 37°C and 5% CO 2 in 24-well culture plates (Greiner, Invitrogen, Breda, The Netherlands) for 5 days. PT and FHA were heat inactivated at 95°C for 15 min to avoid any mitogenicity (21).…”

Section: Methodsmentioning

confidence: 99%

Identification of Pertussis-Specific Effector Memory T Cells in Preschool Children

Rond

Schure

Öztürk

et al. 2015

Clin. Vaccine Immunol.

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dWhooping cough remains a problem despite vaccination, and worldwide resurgence of pertussis is evident. Since cellular immunity plays a role in long-term protection against pertussis, we studied pertussis-specific T-cell responses. Around the time of the preschool acellular pertussis (aP) booster dose at 4 years of age, T-cell memory responses were compared in children who were primed during infancy with either a whole-cell pertussis (wP) or an aP vaccine. Peripheral blood mononuclear cells (PBMCs) were isolated and stimulated with pertussis vaccine antigens for 5 days. T cells were characterized by flow-based analysis of carboxyfluorescein succinimidyl ester (CFSE) dilution and CD4, CD3, CD45RA, CCR7, gamma interferon (IFN-␥), and tumor necrosis factor alpha (TNF-␣) expression. Before the aP preschool booster vaccination, both the proliferated pertussis toxin (PT)-specific CD4؉ and CD8 ؉ T-cell fractions (CFSE dim ) were higher in aP-than in wP-primed children. Post-booster vaccination, more pertussis-specific CD4؉ effector memory cells (CD45RA ؊ CCR7 ؊ ) were induced in aP-primed children than in those primed with wP. The booster vaccination did not appear to significantly affect the T-cell memory subsets and functionality in aP-primed or wP-primed children. Although the percentages of Th1 cytokine-producing cells were alike in aP-and wP-primed children pre-booster vaccination, aP-primed children produced more Th1 cytokines due to higher numbers of proliferated pertussis-specific effector memory cells. At present, infant vaccinations with four aP vaccines in the first year of life result in pertussis-specific CD4 ؉ and CD8 ؉ effector memory T-cell responses that persist in children until 4 years of age and are higher than those in wP-primed children. The booster at 4 years of age is therefore questionable; this may be postponed to 6 years of age.

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“…Peripheral blood mononuclear cells (PBMCs) were isolated from blood as described earlier and frozen (3). After thawing, 3.0 ϫ 10 5 viable cells per well were cultured in AIMV medium (Gibco Invitrogen, Grand Island, NY) containing 5% human AB serum (Harlan Laboratories, Leicestershire, United Kingdom) (AIMVϩ) for 5 days at 37°C and 5% CO 2 in 96-well round-bottomed culture plates (Greiner, Invitrogen, Breda, The Netherlands) and stimulated in triplicate with 2 g/ml inactivated PT, FHA (Kaketsuken, Kumamoto, Japan; endotoxin content, Ͻ 1.17 endotoxin units [EU]/ml), 4 g/ml of recombinant Prn (22), and 5 g/ml pokeweed mitogen (Sigma Chemicals, St. Louis, MO) as a positive control. Nonstimulated (NS) cells served as negative controls.…”

Section: Methodsmentioning

confidence: 99%

T-Cell Responses before and after the Fifth Consecutive Acellular Pertussis Vaccination in 4-Year-Old Dutch Children

Schure¹,

Hendrikx²,

Rond³

et al. 2012

Clin. Vaccine Immunol.

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cImmunization with acellular pertussis vaccine (aP) induces higher specific antibody levels and fewer adverse reactions than does immunization with the whole-cell vaccine (wP). However, antibody levels in infants induced by both types of pertussis vaccines wane already after 1 year. Therefore, long-term T-cell responses upon vaccination might play a role in protection against pertussis. In a cross-sectional study (ISRCTN65428640), we investigated T-helper (Th) cell immune responses in wP-or aP-vaccinated children before and after an aP low-dose or high-dose preschool booster at 4 years of age in The Netherlands. T cells were stimulated with pertussis vaccine antigens. The numbers of gamma interferon-producing cells and Th1, Th2, Th17, and interleukin-10 (IL-10) cytokine concentrations were determined. In addition, pertussis-specific IgE levels were measured in plasma. Children being vaccinated with aP vaccinations at 2, 3, 4, and 11 months of age still showed higher pertussis-specific T-cell responses at 4 years of age than did wP-vaccinated children. These T-cell responses failed to show a typical increase in cytokine production after a fifth aP vaccination but remained high after a low-dose booster and seemed to decline even after a high-dose booster. Importantly, elevated IgE levels were induced after this booster vaccination. In contrast, wP-vaccinated children had only low prebooster T-cell responses, and these children showed a clear postbooster T-cell memory response even after a low-dose booster vaccine. Four high-dose aP vaccinations in infancy induce high T-cell responses still present even 3 years after vaccination and enhanced IgE responses after preschool booster vaccination. Therefore, studies of changes in vaccine dosage, timing of pertussis (booster) vaccinations, and the possible association with local side effects are necessary.

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Hybrid genes over-express pertactin from Bordetella pertussis

Cited by 14 publications

References 50 publications

Purification design and practice for pertactin, the third component of acellular pertussis vaccine, from Bordetella pertussis

Purification design and practice for pertactin, the third component of acellular pertussis vaccine, from Bordetella pertussis

Identification of Pertussis-Specific Effector Memory T Cells in Preschool Children

T-Cell Responses before and after the Fifth Consecutive Acellular Pertussis Vaccination in 4-Year-Old Dutch Children

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