Hybrid dysgenesis in drosophila melanogaster: The frequency and distribution of male recombination events

Eggleston, Paul

doi:10.1038/hdy.1984.21

Cited by 3 publications

(6 citation statements)

References 30 publications

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“…This relationship has also been described previously for intra-chromosome effects (Eggleston, 1984;Exley & Eggleston, 1989). This relationship has also been described previously for intra-chromosome effects (Eggleston, 1984;Exley & Eggleston, 1989).…”

Section: Discussionsupporting

confidence: 85%

“…If P elements, or the action of P transposase, from one chromosome can generate 'distance effects' on another chromosome, where (in the recipient chromosome) do these 'distant' male recombination events occur? See also Eggleston (1984) and Exley & Eggleston (1989). The distribution of male recombination events along the second and third chromosomes, produced by the activity of P elements on chromosomes 3 and 2 respectively, are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

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Chromosome interactions in P-M dysgenic male recombination ofDrosophila melanogaster

Eggleston

Exley

1992

Genet. Res.

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The frequency, distribution and structure of P elements on the second and third chromosomes of Texas 1, a wild-type inbred strain of Drosophila melanogaster, were investigated by in situ hybridization. These autosomes were isolated individually and used as P-element donors to study the frequency and distribution of male recombination events generated on recipient chromosomes which were originally devoid of P sequences. The P-element array of chromosome 2 was shown to generate higher male recombination frequencies on chromosome 3 than vice versa, despite having fewer P factors and fewer P elements in general. This is likely to be due to the presence and distribution of specific P-deletion derivatives, which vary in their ability to repress P mobility. The male recombination generated on recipient chromosomes is associated with the insertion of donated P sequences, but only in a small minority of cases could a novel P-element site be detected at, or near, the recombination breakpoint. The majority of such breakpoints appear to be associated either with unsuccessful P insertion, or with the action of P transposase attracted by P elements newly inserted elsewhere on the recipient chromosome. Recent evidence also suggests that a small proportion of the breakpoints may be associated with the action of P transposase alone. Male recombination breakpoints appear to be distributed effectively at random along the recipient autosomes, and their frequency of occurrence was shown to correlate with the physical length of DNA available between markers, as revealed by the polytene map distance.

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Section: Discussionsupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Chromosome interactions in P-M dysgenic male recombination ofDrosophila melanogaster

Eggleston

Exley

1992

Genet. Res.

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“…The results of an analysis of variance testing the differences between the Cross A and Cross B data are given as follows: N.S., P > 0 0 5 ; *005 > P > 001; **001 > P > 0-001; ••*/>< 0001. levels of sn w destabilization, more embryo lethality and a greater reduction in female fertility. Following the procedure described by Eggleston (1984) and utilizing the crossing scheme in Fig. 1, dysgenic males were produced in which P elements were restricted to the third chromosome.…”

Section: Resultsmentioning

confidence: 99%

“…Following Eggleston (1984) male recombination was assayed among the progeny of 100 individual dysgenic males using the crossing scheme in Fig. 1.…”

Section: (Iii) Measurement Of Male Recombinationmentioning

confidence: 99%

Structure, frequency and distribution of P elements in relation to P—M hybrid dysgenic male recombination inDrosophila melanogaster

Exley

Eggleston

1989

Genet. Res.

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SummaryThe frequency and distribution of P elements were investigated in the third chromosomes of two wild-type strains of Drosophila melanogaster using in situ hybridization of biotinylated probes to the polytene chromosomes. The relationship between these data and the extent of hybrid dysgenesis was determined through assays of egg production, egg hatchability (F2 embryo lethality), snw destabilization and male recombination along the third chromosome. The results suggest that P-element distribution, frequency and structure are all contributory factors in the regulation of hybrid dysgenesis. Texas 6 was shown consistently to be a stronger P strain than Texas 1, eliciting greater reductions in fertility, more extensive snw destabilization and higher frequencies of male recombination. Clustering of male recombination events, arising from pre-meiotic crossing over, was evident among the dysgenic progeny of each strain. Male recombination and snw destabilization were independently distributed among the dysgenic males studied, suggesting that these traits represent separate P-mediated functions. The third chromosome male recombination maps produced by the two strains differed significantly from each other and from the published female meiotic and polytene chromosome maps. Male recombination breakpoints were associated with the original distribution of P sequences in the two strains and the results suggest that this relationship may be closer for potentially complete P factors than for P sequences in general. An analysis of sub-lines derived from individual recombinant males revealed that chromosomal breakpoints could also be associated with novel insertions following P-element transposition.

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“…All FZ progeny were scored for the occurrence of spontaneous second chromosome male recombination at the region between the en and bw loci (woodruff and Thompson 1977;Bencze and Slatko 1984;Eggleston 1984). …”

Section: Male Recombination (Mr) Testmentioning

confidence: 99%

An X-linked genetic factor that affects the activity of glucose-6-phosphate dehydrogenase (G 6 PD) in Drosophila melanogaster: Effect of cytoplasm on its loss from the X chromosome.

Itoh

Hori

1985

The Japanese journal of genetics

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An X-linked regulatory mutation in Drosophila melanogaster which gives rise to constitutive expression of the G 6 PD gene appears to have resulted from insertion of a novel class of transposable genetic elements into the vicinity of the G 6 PD locus. Flies carrying this element exhibit a very high level of G 6 PD activity, and linkage of this element to the X chromosome is stable as long as the mutants are inbred. However, loss of the high-G 6 PD activity trait, probably due to excision of the inserted element, often occurs when the mutant males are mated with females of other strains showing a normal level of G 6 PD activity (hence lacking in the element), but not in the reciprocal crosses. This suggests that the cytoplasm of the mutant might possess some factors which stabilize the regulatory element, and that the element itself might be responsible for the production of this stabilizing factor.

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Hybrid dysgenesis in drosophila melanogaster: The frequency and distribution of male recombination events

Cited by 3 publications

References 30 publications

Chromosome interactions in P-M dysgenic male recombination ofDrosophila melanogaster

Chromosome interactions in P-M dysgenic male recombination ofDrosophila melanogaster

Structure, frequency and distribution of P elements in relation to P—M hybrid dysgenic male recombination inDrosophila melanogaster

An X-linked genetic factor that affects the activity of glucose-6-phosphate dehydrogenase (G 6 PD) in Drosophila melanogaster: Effect of cytoplasm on its loss from the X chromosome.

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