Hybrid Cells Derived from Mouse and Man : Artificial Heterokaryons of Mammalian Cells from Different Species

Harris, Henry; Watkins, J.

doi:10.1038/205640a0

Cited by 579 publications

(112 citation statements)

References 6 publications

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“…Artificially induced fusion has been demonstrated in a number of animal cells and plant and bacterial protoplasts (Harris & Watkins, 1965;Power et al, 1970;Fodor & Alfoldi, 1976). It was reasonable to expect that yeast protoplasts could also be fused without any difficulties.…”

Section: Discussionmentioning

confidence: 99%

Fusion of Yeast Protoplasts Induced by Polyethylene Glycol

Svoboda¹

1978

Journal of General Microbiology

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Protoplasts, prepared from auxotrophic strains of Saccharomyces cerevisiae, Schizosaccharomyces pornbe and Hansenula wingei, were mixed to give intraspecific complementary combinations. Polyethylene glycol (PEG) was added to induce agglutination and fusion. Some of the fused products grew on the surface of solid minimal medium forming large vacuolated bodies. Others reverted to hybrid cells when embedded in solid minimal regeneration medium. The cytological and preliminary genetical analyses suggest a synkaryon formation and integration of genetic markers from parental strains. The frequency of intrageneric fusions assessed from the number of protoplasts growing on the surface of minimal agar was estimated to be 1 to 3 %), while the frequency of hybrid colony formation in regeneration medium was less than 1 (yo.

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Section: Discussionmentioning

confidence: 99%

Fusion of Yeast Protoplasts Induced by Polyethylene Glycol

Svoboda¹

1978

Journal of General Microbiology

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“…This was done essentially as described by Harris & Watkins (1965) using UV-inactivated Sendai virus as the fusing agent and a 10:1 lymphocyte melanoma cell ratio; typically 107 lymphocytes, 106 melanoma cells. The hybrid clones were isolated in HAT medium (Littlefield, 1964).…”

Section: Cell Fusionmentioning

confidence: 99%

Cell fusion segregates progressive growth from metastasis

Sidebottom¹,

Clark²

1983

Br J Cancer

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Summary Cell fusion has been used to analyse the genetic determinants of metastasis at the cellular level. Highly metastatic mouse melanoma cells were fused with diploid mouse lymphocytes and a range of hybrid clones isolated and tested for tumorigenicity and metastatic potential by s.c. injection into newborn, histocompatible, sublethally-irradiated mice. Although almost all clones tested were tumorigenic, most had considerably reduced metastatic potential. This suggests that tumorigenicity and metastasis are determined by different genetic elements. Histological examination of primary tumours produced by metastatic and nonmetastatic hybrid cell lines showed that an essential step in the production of metastases is the separation of tumour cells from the main tumour mass and their movement into the surrounding tissues. The primary tumours of a metastatic hybrid cell line showed local invasiveness whereas those of a non-metastatic cell line did not.

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“…1 Cell fusi'on experiments in the presence of inactivated sendai virus (Harris and Watkins, 1965), showed (Fig. 4) the presence of this small molecular weight fraction in crosses (55-60% fusion) between BSC-1 and tsl4/MNU/2, but it was absent from crosses between BSC-1 and ts14.…”

Section: Resultsmentioning

confidence: 95%