Hybrid Capture II and Polymerase Chain Reaction for Identifying HPV Infections in Samples Collected in a New Collection Medium

Nonogaki, Suely; Wakamatsu, Alda; Filho, Adhemar Longatto; Pereira, Sônia Maria Miranda; Utagawa, Maria Lúcia; FerreiraAlves, Venâncio Avancini; Loreto, Celso di; Maeda, Marina Yoshiê Sakamoto; Lima, Temístocles Pacheco; Roteli-Martins, Cecília Maria; Syrjänen, Karí

doi:10.1159/000326413

Cited by 29 publications

(30 citation statements)

References 14 publications

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“…To screen for the presence of HPV, we used PCR, but avoided nested PCR to minimize chances of false positives. A study by Nonogaki et al (2004) demonstrated that the ability of the two tests to detect HPV is identical; therefore, we feel confident about our PCR results. The PCR amplifications were done with the primers MY11 (5ꞌ-GCMCAGGGWCATAAYAATGG-3ꞌ) and MY09 (5ꞌ-CGTCCMAARGGAWACTGATC-3ꞌ), which are among the most commonly used (Qu et al, 1997) for the amplification of approximately 450-bp fragments of the L1-conserved region of a wide spectrum of HPV genotypes (Bauer and Manos, 1993).…”

Section: Pcr Amplification and Sequencingmentioning

confidence: 59%

Prevalence of human papillomavirus (HPV) type 16 variants and rare HPV types in the central Amazon region

Castro¹,

Farias²,

Borborema-Santos³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Infection by human papillomavirus (HPV) is one of the primary causes of mortality by cancer in northern Brazil. Sexually active women from Manaus, Amazonas, without cytological alterations and women with pre-malignant and malignant cytological alterations were examined for HPV virus, identified via PCR and sequencing. The target region for this study was part of the L1 capsid gene of HPV. Twentythree samples that were PCR-positive were sequenced. Analysis of 336 bp demonstrated a high incidence of high-risk HPV types in the population of Manaus, identified as HPVs 16, 33, 58, 66, 68. HPV type 16 was the most prevalent, presenting two variants similar to the AsianAmerican (AA) and East-Asian type (As) variants. A rare HPV type 13 related to "Heck's disease" was also detected. This preliminary provides important information about the HPV circulating in Amazonas State.

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Section: Pcr Amplification and Sequencingmentioning

confidence: 59%

Prevalence of human papillomavirus (HPV) type 16 variants and rare HPV types in the central Amazon region

Castro¹,

Farias²,

Borborema-Santos³

et al. 2011

Genet. Mol. Res.

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“…Venturoli et al 30 showed 91.7% sensitivity and 95.4% specificity for this method. Most authors have demonstrated high concordance between hybrid capture and PCR, ranging from 76.5% to 90% 30,31,32 . Our study showed 40% concordance between PCR and hybrid capture.…”

Section: Discussionmentioning

confidence: 99%

Utilization of human papillomavirus testing for cervical cancer prevention in a university hospital

et al. 2007

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This study aimed to evaluate the performance and cost of using polymerase chain reaction (PCR) and hybrid capture in the detection of cervical intraepithelial neoplasia (CIN) in patients with cytological abnormalities (ASCUS/low-grade squamous intraepithelial lesion - LSIL), and the feasibility of implementing these methods in Brazil's Unified National Health System (SUS). Colposcopy gave a negative predictive value of 92.86% and efficiency of 87.8% for diagnosing CIN. The sensitivity of PCR and hybrid capture for detecting CIN was 83.33% and 66.67%, respectively, and the negative predictive value for diagnosing CIN2/CIN3 was 100% and 94.74%, respectively. The annual cost for 80 patients was lower when all patients with ASCUS/LSIL were referred for colposcopy than when HPV testing was performed and those with positive results were referred for colposcopy. Therefore, at present, it is financially unfeasible for the National Health System to implement HPV testing to screen patients with cytological abnormalities (ASCUS/LSIL). However, considering that large-scale use might make such methods cheaper, PCR should be the chosen method, since it is less expensive, more sensitive, and has a high negative predictive value.

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“…Therefore, the confirmatory diagnosis of HPV is done only by molecular hybridization methods, of which the polymerase chain reaction (PCR) is the most sensitive (Das et al 2000). A recente study (Nonogaki et al 2004) comparing the performance of HPV DNA detection by PCR and Hybrid Capture II (HCII) found that both tests yielded concordant results in 76.5%. New techniques in primary cervical cancer screening programs have been evaluated.…”

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confidence: 99%

Immune response in cervical dysplasia induced by human papillomavirus: the influence of human immunodeficiency virus-1 co-infection - review

Nicol¹,

Fernandes²,

Bonecini-Almeida³

2005

Mem. Inst. Oswaldo Cruz

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Human immunodeficiency virus (HIV-1) has become an important risk factor for human papillomavirus (HPV) infection and the development of HPV associated lesions inKey words: human papillomavirus -human immunodeficiency virus-1 -cervical intraepithelial neoplasiacervical immunity response -womenAll papillomaviruses have a nonenveloped icosahedral capsid that is 55 nm in diameter and that contains a double-stranded circular DNA genome of about 7900 nucleotide pairs (Howley 1995, Shah & Howley 1995 that codes for at least eight early and two late (capsid) proteins. The expression of viral genes is modulated by an 800-base pair long-control region (LCR) that is epithelial tissue specific and regulated by physiological signals. The products of the early genes E6 and E7 are oncoproteins that destabilize the cellular tumor supressors p53 and pRB (Dyson et al. 1989, Hawley-Nelson et al. 1989.Human papillomavirus (HPV) is currently detected in a large number of mammals and have been shown highly species-specific. HPV subtypes are defined by DNA sequence analysis and represent genotypes. There are over 100 different types of virus; approximately 130 additional isolates represent only partially characterized putative novel genotypes (de Villiers 1997, zur Hausen 1999. Among them, more than 20 types can infect the genital epithelia. In order to be considered a new HPV type, the E6, E7 and L1 gene sequence in the HPV genome has to differ by more than 10% from those of any previously described HPV types. The viral E6 and E7 oncoproteins are essential components in malignant conversion, although, in spite of being necessary, they are not sufficient for the development of the malignant phenotype (revised by zur Hausen 1999).+ Corresponding

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Hybrid Capture II and Polymerase Chain Reaction for Identifying HPV Infections in Samples Collected in a New Collection Medium

Cited by 29 publications

References 14 publications

Prevalence of human papillomavirus (HPV) type 16 variants and rare HPV types in the central Amazon region

Prevalence of human papillomavirus (HPV) type 16 variants and rare HPV types in the central Amazon region

Utilization of human papillomavirus testing for cervical cancer prevention in a university hospital

Immune response in cervical dysplasia induced by human papillomavirus: the influence of human immunodeficiency virus-1 co-infection - review

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