Hybrid C57BL/6J ?? FVB/NJ Mice Drink More Alcohol than Do C57BL/6J Mice

Blednov, Yuri A.; Metten, Pamela; Finn, Deborah A.; Rhodes, Justin S.; Bergeson, Susan E.; Harris, R. Adron; Crabbe, John C.

doi:10.1097/01.alc.0000187605.91468.17

Cited by 49 publications

(67 citation statements)

References 29 publications

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“…C57BL/6J mice have been shown to drink ethanol avidly. Male mice have been reported to consume 9-14 g/kg/day and females 12-18 g/kg/day, when 10% ethanol solution is available under continuous access, free-choice oral selfadministration conditions [37][38][39][40]. In this study, female mice consumed ethanol about 12 g/kg/day at a concentration of 10%, whereas males consumed only about 3 g/kg/day.…”

Section: Discussionmentioning

confidence: 81%

Increase in Free Choice Oral Ethanol Self‐Administration in Catechol‐O‐Methyltransferase Gene‐Disrupted Male Mice

Tammimäki

Forsberg

Karayiorgou

et al. 2008

Basic Clin Pharma Tox

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The effect of catechol-O -methyltransferase ( Comt ) gene disruption on the voluntary oral consumption of water, ethanol (2.5-20%, v/v) and cocaine (0.1-0.8 mg/ml) was studied in the free-choice, two-bottle paradigm in male and female mice. Solutions containing ethanol or cocaine, or tap water were available ad libitum from drinking burettes for 4 weeks. Catechol-O -methyltransferase-deficient male mice consumed significantly more ethanol than their wild-type male littermates. In contrast, female mice did not show genotype differences in the consumption of ethanol solutions. During the cocaine experiment, male mice developed either a side preference or an aversion that obscured cocaine consumption. This pattern of drinking was not dependent on Comt genotype. In female mice, Comt genotype was not associated with cocaine consumption. In conclusion, disruption of Comt gene influenced ethanol consumption in a gender-dependent manner in mice, supporting the hypothesis that low catechol-O -methyltransferase activity is one of the predisposing factors for high alcohol consumption in males.Catechol-O -methyltransferase catalyses the metabolism of catecholamines and other catechols both in the brain and in the peripheral tissues. In striatum, uptake by the dopamine transporter removes dopamine from the synaptic cleft [1][2][3]. However, in brain areas with a low dopamine transporter density (e.g. in the prefrontal cortex), the role of catechol-O -methyltransferase in the control of dopaminergic transmission is more crucial [4][5][6]. Recently, it was shown that in catechol-O -methyltransferase ( Comt ) gene-disrupted mice, elimination of dopamine in prefrontal cortex was two times slower than in wild-type animals, whereas dopamine clearance in the striatum was normal [7]. Prefrontal cortex is involved in certain aspects of cognition [8,9]. It sends projections and modulates dopaminergic transmission in subcortical areas and even dopamine synthesis in the midbrain [10]. These pathways are critically important not only in reward and emotions but also in the development of addiction [11].The common functional Val108/158Met polymorphism is known to control human catechol-O -methyltransferase activity. The Met allele results in a heat-labile enzyme with reduced enzyme activity [12,13]. Comt Val108/158Met polymorphism has been proposed to affect cognitive functions [14,15], but its role in alcoholism remains unclear. Some studies have shown an association between the high catechol-O -methyltransferase activity and alcoholism in males [16]. In contrast, there are reports claiming an association between the low enzyme activity and high alcohol consumption in non-alcoholic males [17] as well as in individuals with type 1 and type 2 alcoholism [18,19]. Several reports also indicate that catechol-O -methyltransferase polymorphism is not linked to alcoholism [20].Previous studies with catechol-O -methyltransferase-deficient mice have shown that the gene disruption has only minor effects on striatal dopamine levels under no...

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Section: Discussionmentioning

confidence: 81%

Increase in Free Choice Oral Ethanol Self‐Administration in Catechol‐O‐Methyltransferase Gene‐Disrupted Male Mice

Tammimäki

Forsberg

Karayiorgou

et al. 2008

Basic Clin Pharma Tox

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“…Although B6 exhibited the highest consumption and preference for all ethanol solutions examined, recent work determined that preference for 10 -30% ethanol solutions was significantly increased over that of B6 in an F1 hybrid cross of B6 and FVB mice (Blednov et al, 2005). This finding appeared to be selective for 2-bottle preference procedures, as the B6 and F1 hybrids did not differ in ethanol consumption with the "drinking in the dark" procedure, where mice had 2 hr of access to a single ethanol bottle containing a 20% ethanol solution (Crabbe and Rhodes, unpublished).…”

Section: Discussionmentioning

confidence: 95%

Voluntary ethanol consumption in 22 inbred mouse strains

Yoneyama

Crabbe

Ford

et al. 2008

Alcohol

Self Cite

274

269

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Inbred strains are genetically stable across time and laboratories, allowing scientists to accumulate a record of phenotypes, including physiological characteristics and behaviors. To date, the C57/C58 family of inbred mouse strains has been identified as having the highest innate ethanol consumption, but some lineages have rarely or never been surveyed. Thus, the purpose of the present experiment was to measure ethanol preference and intake in 22 inbred mouse strains, some of which have never been tested for ethanol consumption. Male and female mice (A/J, BALB/cByJ, BTBR+T tf/tf , BUB/ BnJ, C57BL/6J, C57BLKS/J, C58/J, CZECH/Ei, DBA/2J, FVB/NJ, I/LnJ, LP/J, MA/MyJ, NOD/ LtJ, NON/LtJ, NZB/B1NJ, NZW/LacJ, PERA/Ei, RIIIS/J, SEA/GnJ, SM/J, and 129S1/SvlmJ) were individually housed and given unlimited access in a two-bottle choice procedure to one bottle containing tap water and a second containing increasing concentrations of ethanol (3%, 6%, 10%), 0.2% saccharin, and then increasing concentrations of ethanol (3%, 6%, 10%) plus 0.2% saccharin. Mice were given access to each novel solution for a total of 4 days, with a bottle side change every other day. Consistent with previous studies, C57BL/6J (B6) mice consumed an ethanol dose of > 10g/kg/day while DBA/2J (D2) mice consumed < 2g/kg/day. No strain voluntarily consumed greater doses of ethanol than B6 mice. While the C58 and C57BLKS strains showed high ethanol consumption levels that were comparable to B6 mice, the BUB and BTBR strains exhibited low ethanol intakes similar to D2 mice. The addition of 0.2% saccharin to the ethanol solutions significantly increased ethanol intake by most strains and altered the strain distribution pattern. Strong positive correlations (rs ≥ 0.83) were determined between consumption of the unsweetened versus sweetened ethanol solutions. Consumption of saccharin alone was significantly positively correlated with the sweetened ethanol solutions (rs = 0.62 -0.81), but the correlation with unsweetened ethanol solutions was considerably lower (rs = 0.37 -0.45). These results add new strains to the strain mean database that will facilitate the identification of genetic relationships between voluntary ethanol consumption, saccharin preference, and other phenotypes.

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“…21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 result from both genetic and environmental influences. cHAP mice (Oberlin et al, 2011) or HAD mice (Chester et al, 2003) as well as F1 hybrid mice from the cross of C57BL/6J and FVB/NJ (B6 9 FVB and FVB 9 B6) (Blednov et al, 2005) are a few selectively bred high alcohol preferring strains. On the other hand, the present study reveals that in a population of mice of a given strain, there were variations in voluntary alcohol intake.…”

Section: Discussionmentioning

confidence: 99%