Hybrid Biomaterial with Conjugated Growth Factors and Mesenchymal Stem Cells for Ectopic Bone Formation

Yuan, Xue; Smith, Randall J.; Guan, Huiyan; Ionita, Ciprian N.; Khobragade, P.; Dziak, Rosemary; Liu, Zunpeng; Pang, Manhui; Wang, Changdong; Guan, Guoqiang; Andreadis, Stelios T.; Yang, Shuying

doi:10.1089/ten.tea.2016.0052

Cited by 25 publications

(22 citation statements)

References 51 publications

(71 reference statements)

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“…ALP can be employed to reflect the osteogenic differentiation at the early stage, which is a Zn 2+ metalated glycoprotein. Zn 2+ , as the native cation in ALP, has been demonstrated to promote the stability and activity of ALP .…”

Section: Discussionmentioning

confidence: 99%

Osteogenic activity and antibacterial effect of porous titanium modified with metal‐organic framework films

Chen

Zhang

Huang

et al. 2016

J Biomedical Materials Res

109

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As a new class of crystalline nanoporous materials, metal-organic frameworks (MOFs) have recently been used for biomedical applications due to their large surface area, high porosity, and theoretically infinite structures. To improve the biological performance of titanium, MOF films were applied to surface modification of titanium. Zn-based MOF films composed of zeolitic imidazolate framework-8 (ZIF-8) crystals with nanoscale and microscale sizes (nanoZIF-8 and microZIF-8) were prepared on porous titanium surfaces by hydrothermal and solvothermal methods, respectively. The ZIF-8 films were characterized by powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The nanoZIF-8 film exhibited good biocompatibility, whereas the microZIF-8 film showed obvious cytotoxicity to MG63 cells. Compared to pure titanium and alkali- and heat-treated porous titanium, the nanoZIF-8 film not only enhanced alkaline phosphatase (ALP) activity, extracellular matrix mineralization, and expression of osteogenic genes (ALP, Runx2) in MG63 cells but also inhibited the growth of Streptococcus mutans. These results indicate that MOF films or coatings may be promising candidates for bone tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 834-846, 2017.

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Section: Discussionmentioning

confidence: 99%

Osteogenic activity and antibacterial effect of porous titanium modified with metal‐organic framework films

Chen

Zhang

Huang

et al. 2016

J Biomedical Materials Res

109

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“…Other relevant GFs are, for example, VEGF and FGF9, because of their proangiogenic function that is particularly useful to enhance vessel formation and promote their stabilization and nutrient supply in the first phases of bone regeneration [ 19 , 118 ]. VEGF and FGF9 have been used either alone or in combination, evaluating different methods of GF conjugation to scaffold matrix, such as crosslinking into fibrin gels with Factor XIIIa [ 119 ].…”

Section: Msc-seeded Scaffolds As a Source Of Soluble Factorsmentioning

confidence: 99%

3D Bone Biomimetic Scaffolds for Basic and Translational Studies with Mesenchymal Stem Cells

Sobacchi

Erreni

Strina

et al. 2018

IJMS

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Mesenchymal stem cells (MSCs) are recognized as an attractive tool owing to their self-renewal and differentiation capacity, and their ability to secrete bioactive molecules and to regulate the behavior of neighboring cells within different tissues. Accumulating evidence demonstrates that cells prefer three-dimensional (3D) to 2D culture conditions, at least because the former are closer to their natural environment. Thus, for in vitro studies and in vivo utilization, great effort is being dedicated to the optimization of MSC 3D culture systems in view of achieving the intended performance. This implies understanding cell–biomaterial interactions and manipulating the physicochemical characteristics of biomimetic scaffolds to elicit a specific cell behavior. In the bone field, biomimetic scaffolds can be used as 3D structures, where MSCs can be seeded, expanded, and then implanted in vivo for bone repair or bioactive molecules release. Actually, the union of MSCs and biomaterial has been greatly improving the field of tissue regeneration. Here, we will provide some examples of recent advances in basic as well as translational research about MSC-seeded scaffold systems. Overall, the proliferation of tools for a range of applications witnesses a fruitful collaboration among different branches of the scientific community.

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“…Fibrin hydrogel (FH) can be easily made by mixing fibrinogen with thrombin at 37°C. The mechanical properties of FH can be controlled by changing the concentration of fibrinogen [ 9 ] and can be decorated with bioactive signals making it an ideal scaffold for tissue engineering and regenerative medicine [ 10 – 13 ]. Moreover, fibrinogen and thrombin can be harvested from the patient's own blood, therefore FH can eliminate the immune rejection and viral transmission [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Laminin-111-derived peptide conjugated fibrin hydrogel restores salivary gland function

et al. 2017

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Hyposalivation reduces the patient quality of life, as saliva is important for maintaining oral health. Current treatments for hyposalivation are limited to medications such as the muscarinic receptor agonists, pilocarpine and cevimeline. However, these therapies only provide temporary relief. Therefore, alternative therapies are essential to restore salivary gland function. An option is to use bioengineered scaffolds to promote functional salivary gland regeneration. Previous studies demonstrated that the laminin-111 protein is critical for intact salivary gland cell cluster formation and organization. However, laminin-111 protein as a whole is not suitable for clinical applications as some protein domains may contribute to unwanted side effects such as degradation, tumorigenesis and immune responses. Conversely, the use of synthetic laminin-111 peptides makes it possible to minimize the immune reactivity or pathogen transfer. In addition, it is relatively simple and inexpensive as compared to animal-derived proteins. Therefore, the goal of this study was to demonstrate whether a 20 day treatment with laminin-111-derived peptide conjugated fibrin hydrogel promotes tissue regeneration in submandibular glands of a wound healing mouse model. In this study, laminin-111-derived peptide conjugated fibrin hydrogel significantly accelerated formation of salivary gland tissue. The regenerated gland tissues displayed not only structural but also functional restoration.

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Hybrid Biomaterial with Conjugated Growth Factors and Mesenchymal Stem Cells for Ectopic Bone Formation

Cited by 25 publications

References 51 publications

Osteogenic activity and antibacterial effect of porous titanium modified with metal‐organic framework films

Osteogenic activity and antibacterial effect of porous titanium modified with metal‐organic framework films

3D Bone Biomimetic Scaffolds for Basic and Translational Studies with Mesenchymal Stem Cells

Laminin-111-derived peptide conjugated fibrin hydrogel restores salivary gland function

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