Hybrid assemblies of a symmetric designer protein and polyoxometalates with matching symmetry

Abstract

Novel bioinorganic hybrid materials based on proteins and inorganic clusters have enormous potential for the development of hybrid catalysts that synergistically combine properties of both materials. Here we report the...

Crystal structures of Scone, pseudosymmetric folding of a symmetric designer protein

“…S5). The additive is found at three different positions on the protein surface, and therefore unlikely to bind tightly to any one site, contrary to the strong interactions observed between Pizza6-S and STA Vandebroek et al [2020]. Instead it is bound to the unstructured loops in crystal form a (grown at pH 7.5), stabilizing a fragment of the final disordered repeat.…”

“…Interestingly, this protein could self-assemble from two-or three-bladed fragments, as a trimer or dimer respectively. The Pizza protein was further functionalized to biomineralize a cadmium chloride nanocrystal Voet et al [2015], to form multimeric assemblies by incorporation of coiled-coils Vrancken et al [2020], to bind inorganic polyoxometalates (POMs) that control the crystal packing Vandebroek et al [2020], and to act as a novel hydrolase Clarke et al [2019]. These examples show the potential of the β-propeller fold as a functional building block for a variety of materials.…”

Development and applications of artificial symmetrical proteins

“…In contrast, CPD allows the design of artificial proteins that grow large complexes in the presence of metal ions, which gives a simple means of control over the assembly. In addition, symmetric inorganic complexes such as polyoxometalates can be coordinated by designer proteins as well, potentially leading to hybrid biomaterials combining the advances of biocompatbilility with the catalytic and electronic properties of the inorganic molecules [80] . CPD continues to improve, allowing new and larger structures to be created for applications ranging from bio-catalysis and bio-templating to clinical testing and drug use.…”

Shape and size complementarity induced formation of supramolecular protein assemblies with metal-oxo clusters

“…[21][22][23][24] In previous work, we combined the de novo designed protein, a six-bladed β-propeller protein Pizza6-S with POMs with compatible symmetry in order to explore their potential for creating hybrid assemblies. 25 The protein Pizza6-S was designed with a ring of six histidine residues around the central six-fold symmetrical cavity, creating a positively charged shallow pocket suitable for binding negatively charged polyoxometalate clusters. Dimeric Keggin POM K11[Ce III [PW11O39]2] was shown to bind to two Pizza6-S molecules, holding them together in solution, and altering the crystal packing of the protein into a porous framework in which the protein nodes were connected by POMs.…”