Hyaluronidase in the Treatment of Allergy

McEwen, Lisa M.; Ganderton, M. A.; Wilson, Clare; Black, J.H.

doi:10.1136/bmj.2.5550.507-d

Cited by 6 publications

(4 citation statements)

References 2 publications

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“…The present results give experimental support to the suggestion of McEwen et al [1967] that the ability of commercial samples of hyaluronidase to potentiate clinical hyposensitisation depends on their con tamination with different quantities of /3-glucuronidase.…”

Section: Discussionsupporting

confidence: 77%

“…In an earlier study [McEwen et al, 1967], it was reported that pa tients who had specific allergic diseases of the immediate type were considerably improved by the application of a mixture of hyaluronidase and specific antigen to a scarification site on the forearm. The sample of hyaluronidase used in this study was found to be contaminated with a variable mixture of enzymes, and one of these, /9-glucuronidase, was shown to confer the beneficial therapeutic activity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enzyme-Potentiated Hyposensitisation

McEwen¹,

Starr²

1972

Int Arch Allergy Immunol

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The ability of preparations of hyaluronidase and β-glucuronidase to potentiate the hyposensitising effect of small doses of antigen injected subcutaneously into previously sensitised animals has been investigated in three different models of experimental anaphylaxis. Guinea-pigs were sensitised by egg albumen and repeatedly challenged by exposure to an aerosol of the antigen at weekly intervals. A small dose of antigen by injection increased their pre-convulsion times on subsequent challenge, and this was potentiated by the addition of hyaluronidase and β-glucuronidase. Rats were sensitised by horse serum and challenged 18 days later with intravenous antigen, deaths being noted within 24 h. Ten days after sensitisation, subcutaneous injections of antigen and β-glucuronidase greatly reduced the mortality on subsequent challenge although antigen injected alone had no effect. In mice sensitised by horse serum and challenged by pinnal anaphylaxis, the anamnestic increase in sensitivity produced by a subcutaneous dose of antigen was prevented by β-glucuronidase. Using this model, tolerance was induced by the intravenous injection of antigen and this was prevented by the addition of hyaluronidase.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Enzyme-Potentiated Hyposensitisation

McEwen¹,

Starr²

1972

Int Arch Allergy Immunol

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“…Following the discovery of the method during clinical work [62], and a preliminary unpublished successful double-blind clinical trial in 1967, further detailed development was conducted in experimental animals [63-71. This led to identification of the critical dose range for allergens in the treatment, and of the significance of specific adjunctive chemicals, of which the two mentioned above are the most important. A double-blind verification of the work in hay fever sufferers was conducted [67], and a further small study in food allergy sufferers reported a successful outcome in 1975 [68].…”

Section: Enzyme Potentiated Desensitizationmentioning

confidence: 99%

“Allergy: Conventional and Alternative Concepts”. A Critique of the Royal College of Physicians of London's Report

Downing¹,

Davies²

1992

Journal of Nutritional Medicine

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The content of the Royal College's report is herein reviewed, together with an outline of the background to its publication; its various shortcomings are highlighted. Several techniques of distortion employed in the report are enumerated and discussed, and suggestions are made as to how to avoid the introduction of bias into consensus statements. In conclusion, the article exhorts a united effort on the parts of all concerned to work together in accordance with the ethical principle of seeking to overcome the causes of human suffering.As physicians, a prime aspect of our ethical approach to patients is an overriding concern for their best interests. Intrinsic in this is a vocational desire to alleviate suffering. When it comes to a review of the published literature, this same principle naturally applies. The authors of this critique find themselves in a difficult position: whilst inherently subscribing to the above statement, at the same time they have profound ethical concerns about the way in which the Royal College of Physicians of London have published their report. This has led to a dilemma: how to draw attention to the shortcomings of the report without promoting discord? It was amidst this dilemma that it was decided to submit the critique to the Royal College, and not to publish it, at that time, despite considerable pressure from various quarters to do so.The critique was thus submitted to the college, four months before final publication of the report in 1992, and not circulated to the media, in order to give the college an opportunity to make good the report's shortcomings.When the report was eventually published, however, it still contained many of its original shortcomings. Rather than immediately publish the critique, consideration was given to the possible underlying mechanisms and influences that could have given rise to such a report being published in the first place. As a result of this approach, an article was written by the authors of this critique which addressed the complex issues of logic and motivation that might come into play in any attempt to achieve an 331 J Nutr Environ Med Downloaded from informahealthcare.com by University of Sydney on 01/05/15 For personal use only. J Nutr Environ Med Downloaded from informahealthcare.com by University of Sydney on 01/05/15 For personal use only. J Nutr Environ Med Downloaded from informahealthcare.com by University of Sydney on 01/05/15 For personal use only. J Nutr Environ Med Downloaded from informahealthcare.com by University of Sydney on 01/05/15 For personal use only. J Nutr Environ Med Downloaded from informahealthcare.com by University of Sydney on 01/05/15 For personal use only.

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“…Of these, 26 received the 'very sensitive' grade (a total of 4,800 Protein Nitrogen Units), 10 received the 'moderately sensitive' grade (9,570 PNU total) and six were given the 'mildly sensitive' injections (20,650 PNU total). Ten other control patients were administer ed single placebo injections of 0.9% saline in a depot form [M c Ew en , G anderton, W ilson and Black, 1967].…”

Section: Methodsmentioning

confidence: 99%