Hyaluronic Acid Suppresses the Reduction of .ALPHA.2(VI) Collagen Gene Expression Caused by Interleukin-1.BETA. in Cultured Rabbit Articular Chondrocytes.

Abstract

In order to investigate how alpha2(VI) collagen gene is regulated by inflammatory cytokines in cultured rabbit articular chondrocytes, we examined the effect of interleukin-1beta (IL-1beta) on this collagen mRNA expression. Polylayer cultures of chondrocytes were exposed to IL-1beta (0.1, 1, 10 ng/ml). Quantitative detection of specific mRNA for this collagen was carried out by reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, to investigate the effect of hyaluronic acid (HA) on alpha2(VI) collagen mRNA expression by IL-1beta chondrocytes were exposed to IL-1beta (10 ng/ml) in the presence of HA (0.01, 0.1, 1 mg/ml) with molecular weight of 900 kDa. Chondrocytes were also exposed to IL-1beta (10 ng/ml) in the presence of HA (1 mg/ml) with molecular weights of 200, 900 and 2000 kDa. Alpha2(VI) collagen mRNA expression was decreased significantly in chondrocytes cultured with 1 and 10 ng/ml of IL-1beta. However, the addition of both IL-1beta and HA (0.1, 1 mg/ml) or both IL-1beta and HA (1 mg/ml) with all the molecular weight significantly suppressed these reduced mRNA levels. No tendency for this suppression to depend on the molecular weight was observed. These results suggest that suppression of transcriptional activity for type VI collagen will be associated with the reduction of cartilage matrix tissue and that HA will be associated with the suppression of the effect of IL-1beta.

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“…This formulation, by means of its hydrophilic properties, retain higher amounts of fluid in articular space (36) and is provided by a greater anti-inflammatory activity, as shown by studies on migration of inflammatory cells in the joint and on the reduced PGE2 and bradykinin concentration (32,37,38). Moreover, HMW HA is considered more effective in relieving pain, compared to LMW HA.…”

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“…Outre son rôle mécanique, il agit comme un puissant antiinflammatoire notamment par son action anti-IL-1 freinant la cascade enzymatique délétère, à terme, pour l'ultrastructure cartilagineuse [12,13].…”

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“…Recently, an hyaluronic acid cross -linked preparation , with high molecular weight (6 -7 millions Dalton), has been developed (Migliore et al, 2010). This formulation, by means of its hydrophilic properties, retains higher amounts of fluid in articular space; it is also provided by a greater anti -inflammatory activity, as shown by studies on migration of inflammatory cells in the joint and on reduced Prostaglandin E 2 and bradykinin concentration (Goto et al, 1999;Takahashi et al, 1999). Moreover, high molecular weight hyaluronic acid is considered more effective in relieving pain, compared to low molecular weight hyaluronic acid.…”