Hyaluronic acid: separation and biological implications

Kakehi, Kazuaki; Kinoshita, Mitsuhiro; Yasueda, Shin-ichi

doi:10.1016/s1570-0232(03)00479-3

Cited by 96 publications

(62 citation statements)

References 54 publications

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“…These new histological findings are necessary for further investigations on the involvement of HA production in follicle growth. High-molecular weight HA that accumulates in the ECM has antiangiogenic activity (West & Kumar 1989, Itano 2008 and is found in joints, skin, and cornea (Kakehi et al 2003), where too much disorderly vascularization is undesirable. The tunica adventitia of arteries is the main site of HA accumulation among blood vessels as well.…”

Section: Discussionmentioning

confidence: 99%

Involvement of hyaluronan synthesis in ovarian follicle growth in rats

Takahashi¹,

Tarumi

Ishizuka

2014

Reproduction

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Most of the previous studies on ovarian hyaluronan (HA) have focused on mature antral follicles or corpora lutea, but scarcely on small preantral follicles. Moreover, the origin of follicular HA is unknown. To clarify the localization of HA and its synthases in small growing follicles, involvement of HA in follicle growth, and gonadotropin regulation of HA synthase (Has) gene expression, in this study, perinatal, immature, and adult ovaries of Wistar-Imamichi rats were examined histologically and biochemically and by in vitro follicle culture. HA was detected in the extracellular matrix of granulosa and theca cell layers of primary follicles and more advanced follicles. Ovarian HA accumulation ontogenetically started in the sex cords of perinatal rats, and its primary site shifted to the intrafollicular region of primary follicles within 5 days of birth. The Has1-3 mRNAs were expressed in the ovaries of perinatal, prepubertal, and adult rats, and the expression levels of Has1 and Has2 genes were modulated during the estrous cycle in adult rats and following administration of exogenous gonadotropins in immature acyclic rats. The Has1 and Has2 mRNAs were predominantly localized in the theca and granulosa cell layers of growing follicles respectively. Treatments with chemicals known to reduce ovarian HA synthesis induced follicular atresia. More directly, the addition of Streptomyces hyaluronidase, which specifically degrades HA, induced the arrest of follicle growth in an in vitro culture system. These results indicate that gonadotropin-regulated HA synthesis is involved in normal follicle growth.

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Section: Discussionmentioning

confidence: 99%

Involvement of hyaluronan synthesis in ovarian follicle growth in rats

Takahashi¹,

Tarumi

Ishizuka

2014

Reproduction

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“…Furthermore HA is obtained from different sources and may contain impurities, and current preparations are not characterized thoroughly [66]. New developments in electrophoretic and chromatographic techniques [67] allowing to select HA of well-defined size may motivate future investigations on biological activities and thus result in additional indications. In terms of current clinical use, one is tempted to speculate whether HA may cause adverse reactions upon application of preparations with different polymer sizes owing to its recently discovered function as signaling molecule.…”

Section: Outlook and Perspectivesmentioning

confidence: 99%

Hyaluronic Acid in the Treatment and Prevention of Skin Diseases: Molecular Biological, Pharmaceutical and Clinical Aspects

Weindl

Schaller²,

Schäfer‐Korting³

et al. 2004

Skin Pharmacol Physiol

165

128

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The glycosaminoglycan hyaluronic acid (HA), or hyaluronan, is a major component of the extracellular matrix of skin, joints, eye and many other tissues and organs. In spite of its simple structure, HA demonstrates remarkable rheological, viscoelastic and hygroscopic properties which are relevant for dermal tissue function. Biological activities in skin, however, are also due to its interaction with various binding proteins (hyaladherins). Due to an influence on signaling pathways, HA is involved in the wound-healing process and scarless fetal healing. Increased HA concentrations have been associated with inflammatory skin diseases. In clinical trials, topical application of HA improved wound healing; in particular, acute radioepithelitis, venous leg ulcers or diabetic foot lesions responded to HA treatment. Moreover, as a topical drug delivery system for diclofenac, an HA gel has recently been approved for the treatment of actinic keratoses. Finally, chemical modifications led to new HA derivates and biomaterials, which may be introduced into therapy in the future. Therefore, ongoing research offers new horizons for the therapeutic use of this glycosaminoglycan which has been regarded as an inert structural component until recently.

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“…1 HA is a linear biopolymer with repeating disaccharide units of D-glucuronic acid and N-acetylglucosamine, which are linked alternately by glycosidic linkages. 2 HA is a valuable material, which has received great interest in the medical, cosmetic and food industries.…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of hyaluronic acid concentration in fermentation broth with near-infrared spectroscopy

Dong

Zang

et al. 2014

J. Innov. Opt. Health Sci.

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Hyaluronic acid (HA) concentration is an important parameter in fermentation process. Currently, carbazole assay is widely used for HA content determination in routine analysis. However, this method is time-consuming, environment polluting and has the risk of microbial contamination, as well as the results lag behind fermentation process. This paper attempted the feasibility to predict the concentration of HA in fermentation broth by using near infrared (NIR) spectroscopy in transmission mode. In this work, a total of 56 samples of fermentation broth from 7 batches were analyzed, which contained HA in the range of 2.35-9.69 g/L. Di®erent data preprocessing methods were applied to construct calibration models. The¯nal optimal model was obtained with¯rst derivative using Savitzky-Golay smoothing (9 points window, second-order polynomial) and partial least squares (PLS) regression with leave-one-block-out cross validation. The correlation coe±cient and Root Mean Square Error of prediction set is 0.98 and 0.43 g/L, respectively, which show the possibility of NIR as a rapid method for microanalysis and to be a promising tool for a rapid assay in HA fermentation.

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Hyaluronic acid: separation and biological implications

Cited by 96 publications

References 54 publications

Involvement of hyaluronan synthesis in ovarian follicle growth in rats

Involvement of hyaluronan synthesis in ovarian follicle growth in rats

Hyaluronic Acid in the Treatment and Prevention of Skin Diseases: Molecular Biological, Pharmaceutical and Clinical Aspects

Rapid determination of hyaluronic acid concentration in fermentation broth with near-infrared spectroscopy

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