Hyaluronic acid reagent functional chitosan-PEI conjugate with AQP2-siRNA suppressed endometriotic lesion formation

Abstract

To identify a new drug candidate for treating endometriosis which has fewer side effects, a new polymeric nanoparticle gene delivery system consisting of polyethylenimine-grafted chitosan oligosaccharide (CSO-PEI) with hyaluronic acid (HA) and small interfering RNA (siRNA) was designed. There was no obvious difference in sizes observed between (CSO-PEI/siRNA)HA and CSO-PEI/siRNA, but the fluorescence accumulation in the endometriotic lesion was more significant for (CSO-PEI/siRNA)HA compared with CSO-PEI/siRNA due to the specific binding of HA to CD44. In addition, the (CSO-PEI/siRNA)HA nanoparticle gene therapy significantly decreased the endometriotic lesion sizes with atrophy and degeneration of the ectopic endometrium. The epithelial cells of ectopic endometrium from rat models of endometriosis showed a significantly lower CD44 expression than control after treatment with (CSO-PEI/siRNA)HA. Furthermore, observation under an electron microscope showed no obvious toxic effect on the reproductive organs. Therefore, (CSO-PEI/siRNA)HA gene delivery system can be used as an effective method for the treatment of endometriosis.

A review of drug delivery systems based on nanotechnology and green chemistry: green nanomedicine

“…Furthermore, observations under an electron microscope showed no obvious toxic effects on reproductive organs and confirmed that the (CSO-PEI/ siRNA)HA gene delivery system can be used as a safe and effective way for the treatment of endometriosis. 16 Additional research related to molybdenum NPs includes phosphate glasses incorporating vanadium and molybdenum oxides as useful compounds for the control of drug dissolution and drug release. The modified vancomycin molecules demonstrate greater hydrogen bonding with vanadium-doped glasses and consequently slower drug release over 14 days showing an improved surface modification with drug molecules.…”

Hyaluronic acid–nimesulide conjugates as anticancer drugs against CD44-overexpressing HT-29 colorectal cancer in vitro and in vivo

“…Clearly, significantly higher accumulation of both NIR dye-labeled HA was observed in the liver, which is responsible for the elimination of HA from the circulation. 59,60 Besides, it was found that HA-NIR accumulated in the tumor region, indicating active targeting ability of HA in CD44 receptor overexpressing tumors. 61,62 HA-NIR was also found to be present in spleen and heart.…”

Applications of omics and nanotechnology to improve pig embryo production in vitro

“…In the emerging field of nanotechnology, nanoparticle platforms have also demonstrated great potential for detection, diagnosis, and treatment of human infertility. Most reports have explored the use of nanomaterials for the detection and therapy of reproductive cancers and noncancer conditions, such as uterine leiomyoma (Shalaby et al, ) and endometriosis (Liu et al, ; Zhao et al, ). However, a growing number of studies have applied nanotechnology in reproductive biology to target and sort specific populations of gametes and to optimize gene transfer and editing methodologies (Barkalina, Charalambous, Jones, & Coward, ; Remião et al, ).…”

“…In the emerging field of nanotechnology, nanoparticle platforms have also demonstrated great potential for detection, diagnosis, and treatment of human infertility. Most reports have explored the use of nanomaterials for the detection and therapy of reproductive cancers and noncancer conditions, such as uterine leiomyoma (Shalaby et al, 2016) and endometriosis Zhao et al, 2016).…”