Hyaluronic Acid Promotes and Inhibits Angiogenesis

Kumar, Shant; Kumar, Patricia; Ponting, J; Abdul, Sattar; Rooney, Paul; Pye, David; Hunter, Robin D

doi:10.1007/978-1-4615-3358-0_23

Cited by 7 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results imply that the production of large bundles of collagen fibres when HA oligosaccharides are added to the CAM (Fig. 3) [49] may be due to the production of type I collagen by endothelial cells in the newly forming capillary sprouts. The production of type VIII collagen has also been observed by other workers when endothelial cells are allowed to undergo spontaneous angiogenesis in vitro [36].…”

Section: Hyaluronancollagen Associations In Vitromentioning

confidence: 80%

“…Bur, 0.1 pm clear. 3) [49]. Recently, type VIII collagen has also been proposed to play a role in angiogenesis [36,721.…”

Section: ~4mentioning

confidence: 99%

“…However, if oligosaccharides of two-ten disaccharide units are added to the cells, they exhibit increased proliferation and migration in an apparent emulation of angiogenesis [95]. 3) [49] may be due to the production of type I collagen by endothelial cells in the newly forming capillary sprouts. These results imply that the production of large bundles of collagen fibres when HA oligosaccharides are added to the CAM (Fig.…”

Section: Hyaluronancollagen Associations In Vitromentioning

confidence: 99%

“…We have shown that this stimulation of angiogenesis on the CAM is associated with the appearance of a very marked increase in the number of collagen fibrils when viewed under the electron microscope ( Fig. 3) [49]. The tips of the induced capillary sprouts contain HA but how the breakdown of HA regulates the initiation of angiogenesis is unknown [3].…”

Section: ~4mentioning

confidence: 99%

See 3 more Smart Citations

Inverse relationship between hyaluronan and collagens in development and angiogenesis

Rooney

Kumar

1993

Differentiation

View full text Add to dashboard Cite

The extracellular matrix plays a vital role in regulating normal tissue development and function ~ largely via the specific arrangement of macromolecules such as collagens, proteoglycans, glycosaminoglycans and glycoproteins. Previous reports have concentrated on associations between combinations of collagens/proteoglycans, collagens/glycoproteins and proteoglycans/ glycosaminoglycans whilst little information is available on associations between collagens and free glycosaminoglycans.In this review, we discuss possible associations between collagens and the glycosaminoglycan hyaluronan ; macromolecules which are known to exhibit changes in amount and composition during development and under pathological conditions. We demonstrate two types of collagen/hyaluronan association in vivo : the first, during the formation of extracellular matrix structures where neither collagens nor hyaluronan are degraded, resulting in the regulation of collagen fibrillogenesis, and the second, involving an inverse correlation between collagen synthesis and hyaluronan degradation and vice versa. We suggest that associations between collagens and hyaluronan play an important role in the initiation and maintenance of angiogenesis and put forward a model of cartilage vascularisation which relies on these associations.

show abstract

Section: Hyaluronancollagen Associations In Vitromentioning

confidence: 80%

“…Bur, 0.1 pm clear. 3) [49]. Recently, type VIII collagen has also been proposed to play a role in angiogenesis [36,721.…”

Section: ~4mentioning

confidence: 99%

Section: Hyaluronancollagen Associations In Vitromentioning

confidence: 99%

Section: ~4mentioning

confidence: 99%

See 2 more Smart Citations

Inverse relationship between hyaluronan and collagens in development and angiogenesis

Rooney

Kumar

1993

Differentiation

View full text Add to dashboard Cite

show abstract

“…No correlation has been found between prognosis and amounts of HA in sera from breast-cancer patients (Ponting et al. 1992).…”

Section: Ha Arid Prognosismentioning

confidence: 97%

The role of hyaluronan in tumour neovascularization (review)

Rooney

Kumar

Ponting

et al. 1995

Intl Journal of Cancer

Self Cite

258

204

View full text Add to dashboard Cite

Tumour growth and metastasis are totally dependant upon neovascularization. The target cell for tumour neovascularization is the blood-vessel endothelial cell, and specific angiogenic molecules produced or induced by the tumour are believed to initiate the process. In this report, we review one of these angiogenic molecules, the glycosaminoglycan hyaluronan (HA), which appears to have differing roles in neovascularization depending on its molecular mass. High-molecular-mass HA is anti-angiogenic whereas oligosaccharides of HA, of specific size, actively stimulate endothelial-cell proliferation and migration, 2 of the key events associated with neovascularization, and induce angiogenesis in vivo. We provide details of the action of HA oligosaccharides on endothelial cells, from binding to cell-surface receptors, through activation of signal transduction pathways and gene expression to protein synthesis, cell proliferation and cell migration. We also suggest a model to account for HA of differing molecular mass being present, at different locations, within a single tumour and how this HA aids both general tumour growth and tumour metastasis.

show abstract

Biotinylated hyaluronan: A versatile and highly sensitive probe capable of detecting nanogram levels of hyaluronan binding proteins (hyaladherins) on electroblots by a novel affinity detection procedure

1996

View full text Add to dashboard Cite

Hyaluronan influences cellular proliferation and migration in developing, regenerating and remodelling tissues and in tissues undergoing malignant tumour-cell invasion. The widespread occurrence of hyaluronan-binding proteins indicates that the recognition of hyaluronan is important to tissue organisation and the control of cellular behaviour. A number of extracellular matrix and cellular proteins, which have been termed the hyaladherins, have specific affinities for hyaluronan. These include cartilage link-protein, hyaluronectin, neurocan, versican and aggrecan, which all bind to HA within the extracellular matrix. Cellular receptors for hyaluronan such as CD44 and RHAMM (receptor for hyaluronate-mediated motility) have also been identified. In the present study biotinylated hyaluronan (bHA) was prepared by reacting adipic dihydrazide with a 170 kDa hyaluronan sample using the bifunctional reagent 1-ethyl-3-[3-(dimethylamino) propyl] carbodiimide. The resultant free amine moeity of the hydrazido-hyaluronan was then reacted with biotin succinimidyl ester (sulfo-NHS-biotin) to prepare the bHA. After 4-20% gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electroblotting to nitrocellulose membranes, bHA and avidin alkaline phosphatase conjugate could be used in conjunction with nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate substrates to specifically visualise with high sensitivity (> or = 2 ng), bovine nasal cartilage link-protein, aggrecan hyaluronan binding region, and human fibroblast hyaluronan receptors such as CD-44. Conventional Western blotting using specific monoclonal antibodies to these proteins was also used to confirm the identities of these proteins.

show abstract

Hyaluronic Acid Promotes and Inhibits Angiogenesis

Cited by 7 publications

References 17 publications

Inverse relationship between hyaluronan and collagens in development and angiogenesis

Inverse relationship between hyaluronan and collagens in development and angiogenesis

The role of hyaluronan in tumour neovascularization (review)

Biotinylated hyaluronan: A versatile and highly sensitive probe capable of detecting nanogram levels of hyaluronan binding proteins (hyaladherins) on electroblots by a novel affinity detection procedure

Contact Info

Product

Resources

About