Hyaluronic Acid/Poly(lactic-<I>co</I>-glycolic acid) Core/Shell Fiber Meshes Loaded with Epigallocatechin-3-<I>O</I>-Gallate as Skin Tissue Engineering Scaffolds

Abstract

In this study, hyaluronic acid (HA)/poly(lactic-co-glycolic acid, PLGA) core/shell fiber meshes loaded with epigallocatechin-3-O-gallate (EGCG) (HA/PLGA-E) for application to tissue engineering scaffolds for skin regeneration were prepared via coaxial electrospinning. Physicochemical properties of HA/PLGA-E core/shell fiber meshes were characterized by SEM, Raman spectroscopy, contact angle, EGCG release profiling and in vitro degradation. Biomechanical properties of HA/PLGA-E meshes were also investigated by a tensile strength test. SEM images showed that HA/PLGA-E fiber meshes had a three-dimensional interconnected pore structure with an average fiber diameter of about 1270 nm. Raman spectra revealed that EGCG was uniformly dispersed in the PLGA shell of meshes. HA/PLGA-E meshes showed sustained EGCG release patterns by controlled diffusion and PLGA degradation over 4 weeks. EGCG loading did not adversely affect the tensile strength and elastic modulus of HA/PLGA meshes, while increased their hydrophilicity and surface energy. Attachment of human dermal fibroblasts on HA/PLGA-E meshes was appreciably increased and their proliferation was steadily retained during the culture period. These results suggest that HA/PLGA-E core/shell fiber meshes can be potentially used as scaffolds supporting skin regeneration.

In situ crosslinked electrospun gelatin nanofibers for skin regeneration

“…Several works have been demonstrating the similarity between the mechanical properties of skin tissue and electrospun meshes made by different materials and production strategies [62][63][64]. In terms of mechanical properties, the gelatin electrospun meshes developed here exhibit values generally lower than natural skin, although this behavior can be easily improved by developing hybrid structures to mimic the mechanical properties of the skin.…”

Hyaluronan-Based Nanofibers: Fabrication, Characterization and Application

“…The diameter of HA/PLGA fibers was found to range between 790 nm and 1578 nm (average fiber diameter: 1270 ± 510 nm). After 28 days, the amount of EGCG released from HA/PLGAE meshes achieved a constant rate, reaching about 70% [109].…”

Graphene oxide-stimulated myogenic differentiation of C2C12 cells on PLGA/RGD peptide nanofiber matrices

“…The number of viable cells was found to be directly proportional to the metabolic reaction products obtained in the CCK-8 assay. 19,28 Briefly, the C2C12 myoblasts were seeded at a density of 5 × 10 4 cells/ml and incubated for 24 hours. Subsequently, the cells were treated with increasing concentrations (0 to 500 μg/ml) of GO and incubated with a CCK-8 solution for the last 2 hours of the culture period (24 hours) at 37ºC in the dark.…”

“…Until now, electrospun matrices composed of a wide variety of biodegradable copolymers have been extensively developed for tissue engineering and regeneration 6,19,20 ; however, their use in combination with GO is novel. In the present study, nanofiber matrices composed of PLGA and M13 phage with RGD peptide displayed on its surface (RGD peptide-M13 phage) were prepared via electrospinning.…”