Hyaluronic acid on collagen membranes: An experimental study in rats

Silva, Edson Costa e; Omonte, Sheyla Viana; Martins, Alessandro Gomides Veiga; Castro, Henry; Gomes, Hayder Egg; Zenóbio, Élton Gonçalves; Oliveira, Peterson Antônio Dutra; Horta, Martinho Campolina Rebello; Souza, Paulo Eduardo Alencar

doi:10.1016/j.archoralbio.2016.10.016

Cited by 21 publications

(20 citation statements)

References 36 publications

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“…Histological findings have shown that the immersion in a cross‐linked HA gel did not interfere with the structure and degradation of the collagen membranes or soft tissue invasion into it. Our findings are in line with the results from another study in normoglycemic rats showing that HA did not interfere with tissue integration and structural degradation of collagen membranes …”

Section: Discussionsupporting

confidence: 92%

“…Our findings are in line with the results from another study in normoglycemic rats showing that HA did not interfere with tissue integration and structural degradation of collagen membranes. 28 On one hand, the present study has shown that immersion in HA gel resulted in delayed degradation of the CM in uncontrolled STZ-induced diabetic rats. On the other hand, this effect was not demonstrated in the normoglycemic group, since HA-and PBSimmersed CMs showed no statistically significant differences regarding residual collagen membrane content and thickness.…”

Section: Discussionmentioning

confidence: 52%

“…Immersion of CM in HA has been recently suggested as a novel approach aiming to decrease its resorption rate. This approach has been shown not to interfere with tissue integration and structural degradation of various CMs . However, at present, it is unknown to what extent CM degradation may be influenced by its immersion in HA solution under a condition mimicking a medically compromised situation with an increased inflammatory level such as diabetes.…”

Section: Introductionmentioning

confidence: 99%

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Hyaluronic acid slows down collagen membrane degradation in uncontrolled diabetic rats

Eliezer

Sculean

Miron

et al. 2019

J of Periodontal Research

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Aim To examine the in vitro biokinetics of hyaluronic acid (HA) from a collagen membrane (CM) and to evaluate the in vivo effect of immersion of the CM in HA solution on its degradation in streptozotocin (STZ)‐induced diabetes conditions in a rat calvaria subcutaneous model. Background CM degradation is accelerated in uncontrolled diabetic rats. Immersion of CM in HA has been suggested to decrease their resorption rate without interfering with their tissue integration and structural degradation. However, it is unknown to what extent CM degradation may be influenced by its immersion in HA solution under a condition mimicking a medically compromised situation with an increased inflammatory level such as diabetes. Materials and Methods CMs were soaked in cross‐linked HA. Protein adsorption and the HA release were quantified by ELISA. Diabetes was induced in sixteen rats, while 16 healthy rats served as control. CM was prepared and labeled prior to implantation with Biotin. Seventeen CM were immersed in HA and 17 CM in PBS. In each animal, one test or one control disk was implanted. In order to compare the collagen content, two similar non‐implanted CM were used as baseline. Fourteen days after surgery, thirty‐two animals were sacrificed. The entire calvaria including the skin above, was chemically fixed, decalcified, and embedded in paraffin. Five‐μm‐thick sections were analyzed histologically and histomorphometrically using H&E and avidin‐peroxidase staining. Results The in vitro results demonstrated that the CM adsorbed roughly 80% of the total HA content. After 10 days, 36.3% of the initial HA remained on the CM. The in vivo results demonstrated that diabetes significantly reduced the thickness of the CM, while HA had a significant effect on keeping the membrane thickness. HA increased the residual collagen content in the diabetic group (P < 0.0001) but no such effect was observed in the healthy group. Conclusion Immersion of CM in HA prior to the implantation delays membrane degradation in uncontrolled diabetic compared with normoglycemic rats.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hyaluronic acid slows down collagen membrane degradation in uncontrolled diabetic rats

Eliezer

Sculean

Miron

et al. 2019

J of Periodontal Research

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“…While this implies loss of integrity as a barrier, at the same time the membrane was able to support the bone regeneration process and to create an environment that promoted the deposition and mineralization of the bone matrix, with blood vessels penetrating throughout the membrane at 30 days. A recent study in a similar calvaria defect model also confirmed that, at 30 days of healing, the collagen membrane network is progressively invaded by areas of bone formation and presents a dense infiltrate of leukocytes and fibroblast‐like cells …”

Section: Discussionmentioning

confidence: 62%

Degradation pattern of a porcine collagen membrane in an in vivo model of guided bone regeneration

Calciolari

Ravanetti

Strange

et al. 2018

J of Periodontal Research

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The collagen membrane investigated was biocompatible and able to promote bone regeneration. However, pronounced signs of degradation were observed starting from day 30. Since successful regeneration is obtained only when cell occlusion and space maintenance exist for the healing time needed by the bone progenitor cells to repopulate the defect, the suitability of collagen membranes in cases where long-lasting barriers are needed needs to be further reviewed.

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“…Ideally, the biomaterials should be radiolucent, so that bone formation in the defect can be detected and even monitored by μCT. Pioneer knowledge on the osteoconductive properties of collagen membranes in rat calvaria (Silva et al., ; Song et al., ) and maxillary defects (Taguchi et al., ) has been generated. The collagen membrane used here is composed of porcine type I collagen and type III collagen and has a bilayer structure with a dense and a spongy layer (Bio‐Gide).…”

Section: Introductionmentioning

confidence: 99%

Bone‐conditioned medium modulates the osteoconductive properties of collagen membranes in a rat calvaria defect model

Küchler

Rybaczek

Dobask

et al. 2018

Clinical Oral Implants Res

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Hyaluronic acid on collagen membranes: An experimental study in rats

Abstract: The results suggest that HA did not interfere with tissue integration and structural degradation of BioGide and Osseoguard™ membranes.

Cited by 21 publications

References 36 publications

Hyaluronic acid slows down collagen membrane degradation in uncontrolled diabetic rats

Hyaluronic acid slows down collagen membrane degradation in uncontrolled diabetic rats

Degradation pattern of a porcine collagen membrane in an in vivo model of guided bone regeneration

Bone‐conditioned medium modulates the osteoconductive properties of collagen membranes in a rat calvaria defect model

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