Hyaluronic acid improves frozen-thawed sperm quality and fertility potential in rooster

Lotfi, Saied; Mehri, M.; Sharafi, Mohsen; Masoudi, Reza

doi:10.1016/j.anireprosci.2017.07.018

Cited by 46 publications

(34 citation statements)

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“…Avian sperm are particularly susceptible to oxidative stress [6], though reactive oxygen species (ROS), in physiological quantities, are necessary for important sperm events leading to successful fertilization [7]. In sperm, oxidative stress disturb motility and mitochondrial activity [8]; induces lipid peroxidation of the membrane [9]; and the oxidation and DNA fragmentation [10].…”

Section: Introductionmentioning

confidence: 99%

Effect of crocin and naringenin supplementation in cryopreservation medium on post-thawed rooster sperm quality and expression of apoptosis associated genes

Mehdipour

Kia

Najafi

2019

Preprint

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21The aim of our research was to examine the effects of crocin (0.5 (C0.5), 1 (C1) and 1.5 (C1.5) 22 mM) and naringenin (50 (N50), 100 (N100) and 150 (N150) µM) in cryopreservation extender 23 for freezing rooster semen. Sperm motility, viability, abnormalities, membrane integrity, 24 mitochondrial activity, apoptosis status, lipid peroxidation (LP), GPX, SOD, TAC, the mRNA 25 expression of pro-apoptotic (CASPASE 3) and anti-apoptotic (Bcl-2) genes, fertility and 26 hatchability rate were investigated following freeze-thawing. C1 and N100 resulted in the higher 27 (P < 0.05) total motility and progressive motility in comparison to the control group. C1 and 28 N100 improved viability, membrane integrity and reduced lipid peroxidation. We found much 29 higher values for mitochondria activity with C1 and N100 respect to the control group. The C1 30 and N100 showed lower percentages of early apoptosis when compared with control group. 31Also, C1 and N100 had higher TAC when compared with control group. The mRNA expression 32 of BCL-2 in the C1 and N100 group were significantly higher than that of other treatments. The 33 expression of CASPASES 3 was significantly reduced in C1 and N100 group (P < 0.05) when 34 compared to control group. Significantly higher percentage of fertility and hatching rate were 35 observed in C1 and N100 compared to the control group. In conclusion, crocin at 1 mM and 36 naringenin at 100 µM seem to improve the post-thawing rooster semen quality, fertility and 37 could protect the sperm against excessive ROS generation by reducing the pro-apoptotic 38 (CASPASE 3) and increasing anti-apoptotic (Bcl-2) genes. 39 40 41 3 42 1. Introduction 43 Despite its utilization over 70 years ago [1], cryopreservation of bird sperm causes low 44 fertility, which limits its applying in genetic stock preservation [2]. Cryopreservation causes 45 harmful effects on sperm that decrease sperm viability and motility [3-5]. Avian sperm are 46 particularly susceptible to oxidative stress [6], though reactive oxygen species (ROS), in 47 physiological quantities, are necessary for important sperm events leading to successful 48 fertilization [7]. In sperm, oxidative stress disturb motility and mitochondrial activity [8]; 49 induces lipid peroxidation of the membrane [9]; and the oxidation and DNA fragmentation [10]. 50Adding antioxidant compounds to the freezing medium is known as one of the ways to defeat 51 the deleterious effects of ROS on sperm fertility after thawing, because it blocks or inhibits 52 oxidative stress. Antioxidants provide a positive effect on semen, leading to an improvement in 53 some sperm parameters containing motility and membrane integrity [11][12][13]. 54Naringenin is known as a natural flavonoid that has been studied for some of the most 55 prominent properties containing antioxidant, antiproliferative, anti-inflammatory, and 56 antimutagenic ones [14]. It was observed in previous experimental studies that naringain protects 57 the cells from lead and arsenic-induced oxidative damage [1...

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Section: Introductionmentioning

confidence: 99%

Effect of crocin and naringenin supplementation in cryopreservation medium on post-thawed rooster sperm quality and expression of apoptosis associated genes

Mehdipour

Kia

Najafi

2019

Preprint

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“…Our results showed that rooster semen supplementation with quercetin improved sperm parameters such as forward progressive motility, viability and plasma membrane integrity during hydrogen peroxide‐induced oxidative toxicity. Protective effects of substances with antioxidant properties on rooster sperm motility and viability were demonstrated (Eslami et al., ; Lotfi et al., ; Mirzaei Rad, Eslami, & Ghaniei, ). Moreover, the positive association between progressive motility and fertility following insemination was shown in avian (Fattah et al., ; Lotfi et al., ; Moghbeli et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Protective effects of substances with antioxidant properties on rooster sperm motility and viability were demonstrated (Eslami et al., ; Lotfi et al., ; Mirzaei Rad, Eslami, & Ghaniei, ). Moreover, the positive association between progressive motility and fertility following insemination was shown in avian (Fattah et al., ; Lotfi et al., ; Moghbeli et al., ). Contradictory results were reported about quercetin effects on sperm characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…A previous study showed that endogenous antioxidant capacity of semen is not sufficient to conquer oxidative damage during storage (Aurich, Schönherr, Hoppe, & Aurich, ). Semen enrichment with various compounds and antioxidants ameliorate detrimental effects of ROS and lipid peroxidation during chilled storage or after freeze–thawing process in different species, including boar (Funahashi & Sano, ), bull (Munsi, Bhuiyan, Majumder, & Alam, ), ram (Maxwell & Stojanov, ), stallion (Aurich et al., ), rooster (Eslami, Ghaniei, & Mirzaei Rad, ; Lotfi, Mehri, Sharafi, & Masoudi, ; Moghbeli et al., ) and cat (Baran et al., ) semen.…”

Section: Introductionmentioning

confidence: 99%

“…Semen enrichment with various compounds and antioxidants ameliorate detrimental effects of ROS and lipid peroxidation during chilled storage or after freeze-thawing process in different species, including boar (Funahashi & Sano, 2005), bull (Munsi, Bhuiyan, Majumder, & Alam, 2007), ram (Maxwell & Stojanov, 1996), stallion (Aurich et al, 1997), rooster Lotfi, Mehri, Sharafi, & Masoudi, 2017;Moghbeli et al, 2016) and cat (Baran et al, 2009) semen.…”

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Quercetin attenuates H₂O₂‐induced toxicity of rooster semen during liquid storage at 4°C

Ghaniei

Eslami

Hashem

et al. 2019

Animal Physiology Nutrition

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Current study was carried out to examine the protective effects of quercetin against toxicity induced by hydrogen peroxide in rooster semen in vitro. Semen samples were collected from ten roosters (Ross 308 broiler breeder males, 32 weeks old) twice a week by abdominal massage method. Samples with ≥70% progressive motility were selected, pooled, diluted and used for the study. Experimental groups consisted of negative control, control that received solvent of quercetin, H2O2 (40 μM) and combination groups which incubated with constant dose of H2O2 (40 μM) plus various levels of quercetin (20, 40 and 80 μM). Measurement of total hydroperoxide (HPO), malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (TAC) and superoxide dismutase activity as well as routine sperm tests were done at 0, 24 and 48 hr of storage at 4°C. Results revealed that exposure to hydrogen peroxide significantly increased HPO (138.43 ± 7.32 vs. 66.08 ± 3.97 μmol/g protein), MDA (7.21 ± 0.08 vs. 5.71 ± 2.16 μmol/g protein) and NO (0.367 ± 0.013 vs. 0.215 ± 0.011 μmol/g protein) levels and decreased sperm progressive motility (27.28 ± 1.21 vs. 47.49 ± 1.29%), and amounts of TAC (11.49 ± 0.39 vs. 15.70 ± 0.79 mmol/g protein) compared to control at 24 hr (p < 0.05). Changes at mentioned variables were repeated at 48 hr of storage. Also, co‐administration of quercetin (especially at 40 and 80 μM) with hydrogen peroxide restored the toxic effects of hydrogen peroxide on rooster semen parameters such as primary and secondary lipid peroxidative indicators and other evaluated variables. The study concluded that rooster semen enrichment with quercetin would protect lipid peroxidative and nitrosative hydrogen peroxide‐mediated damage during cold liquid storage of rooster semen.

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Proteomic analysis and microtubule dynamicity of human sperm in electromagnetic cryopreservation

Gholami

Ghaffari

Shahverdi

et al. 2018

J of Cellular Biochemistry

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The proteomic changes, microtubule dynamicity, and quality parameters of human sperm were investigated during cryopreservation in an extremely low electromagnetic field (ELEF) condition. Semen samples were obtained from 210 healthy individuals with normospermia and then were divided into three experimental groups: fresh control, frozen control, and frozen ELEF group. Shotgun proteomics was performed to assess the identification of microtubule proteins of the sperm in experimental groups. Microtubule dynamicity, secondary, and tertiary structure modifications of tubulins, characteristics of transmission electron microscopy of sperm as well as sperm quality parameters were evaluated. The expression ratios of α- and β-tubulins were significantly increased after cryopreservation compared with fresh control while this ratio was not significantly different in frozen ELEF group. The expression ratio of tubulin polymerization-promoting protein was significantly decreased after cryopreservation compared with fresh control. The length, width, and the activity of microtubule, secondary, and tertiary structures of tubulins, motility, and the viability of the sperm were decreased in frozen control as compared with fresh control. The microtubule activity, secondary, and tertiary structures of sperm tubulin in frozen ELEF group were higher than frozen control. Transmission electron microscopy of microtubules showed that the size of the width and length of the microtubules in frozen ELEF group were greater than frozen control. Motility, viability, and reactive oxygen species levels were improved in frozen ELEF group when compared with frozen control. While the microtubule dynamicity of the sperm was affected by the cryopreservation, this trait was improved during the electromagnetic cryopreservation resulted in better motility and viability.

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Hyaluronic acid improves frozen-thawed sperm quality and fertility potential in rooster

Cited by 46 publications

References 44 publications

Effect of crocin and naringenin supplementation in cryopreservation medium on post-thawed rooster sperm quality and expression of apoptosis associated genes

Effect of crocin and naringenin supplementation in cryopreservation medium on post-thawed rooster sperm quality and expression of apoptosis associated genes

Quercetin attenuates H₂O₂‐induced toxicity of rooster semen during liquid storage at 4°C

Proteomic analysis and microtubule dynamicity of human sperm in electromagnetic cryopreservation

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Hyaluronic acid improves frozen-thawed sperm quality and fertility potential in rooster

Cited by 46 publications

References 44 publications

Effect of crocin and naringenin supplementation in cryopreservation medium on post-thawed rooster sperm quality and expression of apoptosis associated genes

Effect of crocin and naringenin supplementation in cryopreservation medium on post-thawed rooster sperm quality and expression of apoptosis associated genes

Quercetin attenuates H2O2‐induced toxicity of rooster semen during liquid storage at 4°C

Proteomic analysis and microtubule dynamicity of human sperm in electromagnetic cryopreservation

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Product

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Quercetin attenuates H₂O₂‐induced toxicity of rooster semen during liquid storage at 4°C