Hyaluronic acid hydrogel in the treatment of osteoarthritis

Barbucci, Rolando; Lamponi, Stefania; Borzacchiello, Assunta; Ambrosio, Luigi; Fini, Milena; Torricelli, Paola; Giardino, Roberto

doi:10.1016/s0142-9612(02)00194-1

Cited by 176 publications

(145 citation statements)

References 32 publications

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“…Therefore, these findings suggest, that sodium hyaluronate stimulated the proliferation of chondrocytes, preferably, or could be derived from the inefficiency of this drug to reduce the severity of DJD (Table 2), which usually leads to the stimulation of cell proliferative response in a compensatory effort. This increase of cellular density in the articular cartilage of the animals submitted to the treatment with hyaluronic acid was also described for Guidolin et al (2001), Barbucci et al (2002) and Melo et al (2008). Inhibition of apoptosis may be another responsible mechanism which resulted in higher numbers of chondrocytes in the treatment with sodium hyaluronate (Takahashi et al, 2000).…”

Section: Resultssupporting

confidence: 77%

“…Finally, in spite of the number of animals indicated by statistics, these results should be undertaken with caution due the small number of animals under evaluation. Morphological findings indicating a lesser intensity of changes in injured cartilage of animals treated with sodium hyaluronate were reported by Guidolin et al (2001) and Barbucci et al (2002). However, in this study, the mean score of the group treated with sodium hyaluronate was greater than those from the others groups (Table 2).…”

Section: Resultssupporting

confidence: 58%

“…The main goals of medical therapy for DJD are to regenerate the cartilage and maintain the joint function, relieving pain and decreasing inflammation (Hanson, 1996;Barbucci et al, 2002). Traditionally, therapeutic objectives are reached through surgical and nonsurgical means.…”

Section: Introductionmentioning

confidence: 99%

“…The effects of hyaluronic acid have been related to the increased synthesis by synoviocytes, inhibition of inflammation, improvement in chondrocyte density and metabolism, inhibition of apoptosis, prevention of degenerative injuries and improved of repairing process of the extracelular matrix (Smith and Gosh, 1987;Takahashi et al, 2000;Guidolin et al, 2001;Barbucci et al, 2002). However, some reports have shown no deleterious effects (Smith et al, 1998;Melo et al, 2003;Biasi et al, 2005;Melo et al, 2008) Therefore, the objective of this study was to investigate the mechanisms of action of sodium hyaluronate and chondroitin sulfate in articular cartilage in dogs undergoing DJD.…”

Section: Introductionmentioning

confidence: 99%

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Effects of chondroitin sulfate and sodium hyaluronate on chondrocytes and extracellular matrix of articular cartilage in dogs with degenerative joint disease

Gonçalves¹,

Melo²,

Gomes³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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Samples of articular cartilage of femur, tibia and patella of 15 dogs with experimentally induced degenerative joint disease (DJD) were microscopically analyzed. Animals were distributed into three groups (n=5): the control group received no medication; the second group was treated with chondroitin sulfate and the third received sodium hyaluronate. Samples were processed and stained with HE and toluidine blue for morphological evaluation. The metabolic and proliferative activity of the chondrocytes was evaluated by the measurement of nucleolar organizer regions (NORs) after impregnation by silver nitrate. Significant differences were not observed (P>0.05) in the morphology among the groups, however, the group treated with sodium hyaluronate had a higher score suggesting a trend to a greater severity of the lesions. Significant differences were not observed (P>0.05) in the measurement of NORs, cells and NORs/cells among the groups. Although differences were not significant, sodium hyaluronate group showed higher NOR and cell counts which suggested an increase of the proliferation rate of chondrocytes. In addition, a higher NOR/cell ratio in the group treated with chondroitin sulfate suggested that this drug may have stimulated the metabolic activity of the chondrocytes, minimizing the lesions resulting from DJD.

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Section: Resultssupporting

confidence: 77%

Section: Resultssupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of chondroitin sulfate and sodium hyaluronate on chondrocytes and extracellular matrix of articular cartilage in dogs with degenerative joint disease

Gonçalves¹,

Melo²,

Gomes³

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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“…Many researchers and companies have focused on the development of injectable hydrogels for different kinds of tissues (Barbucci et al, 2002;Sakai et al, 2015;Varma et al, 2014;Su et al, 2010). Injectable hydrogels can be maintained in the liquid state before injection and harden in situ after transplantation in vivo.…”

Section: Mh Hu Et Almentioning

confidence: 99%

In situ forming oxidised hyaluronic acid/adipic acid dihydrazide hydrogel for prevention of epidural fibrosis after laminectomy

Yang²,

Sun

et al. 2017

eCM

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Post-operative epidural fibrosis is a biological response after laminectomy that may lead to clinical symptoms, such as radicular pain. An ideal material for prevention of epidural fibrosis should be able to inhibit fibroblast adhesions and reduce formation of scar tissue. An injectable hydrogel would be the material of choice for this purpose, since it could fill an irregular surgical defect completely, gelate in situ and be delivered in a minimally-invasive manner. The objective of this study was to evaluate, in vitro and in vivo, the cytocompatibility and anti-adhesive effect of an oxidised hyaluronic acid/adipic acid dihydrazide (oxi-HA/ADH) hydrogel. Different cell types present in the spine were used to test the cytocompatibility of the hydrogel. The hydrogel extraction medium had no deleterious effects on neural cells (PC-12), but reduced fibroblasts viability (NIH/3T3). Although the hydrogel did not change the release of lactate dehydrogenase from myoblasts (C2C12) and Schwann cells (RSC96), the extraction medium concentration slightly affected the mitochondrial activity of these two cell types. qPCR showed that the hydrogel down-regulated S100a and P4hb expression in NIH/3T3 cells, supporting the hypothesis that the hydrogel might inhibit fibroblast activity. The animal study showed a reduction of scar tissue formation as well as severity of adhesion between scar tissue and the dura mater in a rat laminectomy model. Superficially, the peel-off test showed significantly decreased tenacity. In conclusion, the oxi-HA/ADH hydrogel is a promising injectable and thermosensitive material for prevention of post-operative epidural fibrosis.

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