Hyaluronic Acid-Conjugated with Hyperbranched Chlorin e6 Using Disulfide Linkage and Its Nanophotosensitizer for Enhanced Photodynamic Therapy of Cancer Cells

Jung, Shin; Jung, Seunggon; Kim, Doo Man; Lim, Sang Chul; Shim, Yong Ho; Kwon, Hanjin; Kim, Do Hoon; Lee, Chang-Min; Kim, Byung Hoon; Jeong, Young‐Il

doi:10.3390/ma12193080

Cited by 13 publications

(21 citation statements)

References 54 publications

(125 reference statements)

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“…In fact, some evidence now suggests that CD44 acts as a receptor for proteolytically-modified HA and may be responsible for the modulation of intracellular signaling associated with increased proliferation and invasive capacity [ 17 , 26 , 27 ]. However, because the production of proteolytic enzymes with their cognate targets may be tightly regulated within these tumors, administration of intact, exogenous HA and the delivery of chemotherapeutic agents conjugated with HA has been sufficient in clinical studies to improve patient outcomes and reduce metastatic phenotypes in many cancer types [ 17 , 18 , 28 , 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Hyaluronic Acid to Modulate Oral Squamous Cell Carcinoma Growth In Vitro

Ringer

Morrison

Kingsley

2020

JFB

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Introduction: Previous studies have demonstrated that glycosaminoglycan hyaluronic acid (HA) is capable of mediating oral tumor growth. Some clinical evidence has suggested reduced HA expression predicts poor cancer prognosis and that HA-chemotherapy conjugates may function synergistically to inhibit oral tumor growth. Other studies have found conflicting results that suggest enhanced CD44-HA-mediated growth and proliferation. Due to the lack of clarity regarding HA function, the primary goal of this study was to investigate the effects of HA using well-characterized oral cancer cell lines. Methods: Using several commercially available oral squamous cell carcinoma lines (and a normal non-cancerous control), 96-well growth and viability assays were conducted using HA (alone and in combination with chemotherapeutic agents paclitaxel and PD98059). Results: Different results were observed in each of the cell lines evaluated. HA induced small, non-significant changes in cellular viability among each of the cell lines within a narrow range (1–8%), p = 0.207. However, HA induced differing effects on growth, with minimal, non-significant changes among some cell lines, such as SCC4 (+1.7%), CCL-30 (−2.8%), and SCC15 (−2.5%), p = 0.211 and more robust inhibition among other cell lines, SCC9 (−24.4%), SCC25 (−36.6%), and CAL27 (−47.8%), p = 0.0001. Differing effects were also observed with growth and viability under concomitant administration of HA with PD98059 or paclitaxel. Further analysis of these data revealed strong inverse (Pearson’s) correlations between initial baseline growth rate and responsiveness to HA administration, ranging from R = −0.27 to R = −0.883. Conclusion: The results of this study revealed differing responses to HA, which may be inversely correlated with intrinsic characteristics, such as the baseline growth rate. This may suggest that the more rapidly growing cell lines are more responsive to combination therapy with hyaluronic acid; an important finding that may provide insights into the mechanisms responsible for these observations.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Hyaluronic Acid to Modulate Oral Squamous Cell Carcinoma Growth In Vitro

Ringer

Morrison

Kingsley

2020

JFB

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“…Shin Jung et al synthesized a kind of Ce6tetraHA nanocomposite and detected the fluorescence intensity of the drug on mouse CRC cell line HCT116. The drug could be delivered to the tumor site in the way of redox sensitivity and CD44 sensitivity, making the fluorescence intensity of tumor site significantly higher than that of other organs and tissues 130 .…”

Section: Clinical Application Of Ps In Crcmentioning

confidence: 99%

Application of phototherapeutic-based nanoparticles in colorectal cancer

Yan¹,

Wang²,

Jiang³

et al. 2021

Int. J. Biol. Sci.

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Colorectal cancer (CRC) is the third most commonly diagnosed malignancy and the second leading cause of cancer death, which accounts for approximately 10% of all new cancer cases worldwide. Surgery is the main method for treatment of early-stage CRC. However, it is not effective for most metastatic tumors, and new treatment and diagnosis strategies need to be developed. Photosensitizers (PSs) play an important role in the treatment of CRC. Phototherapy also has a broad prospect in the treatment of CRC because of its low invasiveness and low toxicity. However, most PSs are associated with limitations including poor solubility, poor selectivity and high toxicity. The application of nanomaterials in PSs has added many advantages, including increased solubility, bioavailability, targeting, stability and low toxicity. In this review, based on phototherapy, we discuss the characteristics and development progress of PSs, the targeting of PSs at organ, cell and molecular levels, and the current methods of optimizing PSs, especially the application of nanoparticles as carriers in CRC. We introduce the photosensitizer (PS) targeting process in photodynamic therapy (PDT), the damage mechanism of PDT, and the application of classic PS in CRC. The action process and damage mechanism of photothermal therapy (PTT) and the types of ablation agents. In addition, we present the imaging examination and the application of PDT / PTT in tumor, including (fluorescence imaging, photoacoustic imaging, nuclear magnetic resonance imaging, nuclear imaging) to provide the basis for the early diagnosis of CRC. Notably, single phototherapy has several limitations in vivo , especially for deep tumors. Here, we discuss the advantages of the combination therapy of PDT and PTT compared with the single therapy. At the same time, this review summarizes the clinical application of PS in CRC. Although a variety of nanomaterials are in the research and development stage, few of them are actually on the market, they will show great advantages in the treatment of CRC in the near future.

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“…The uptake of hyaluronic acid (HA) conjugated nanosystem was proven to be specific to MDA-MB-231 cells and provided an efficient strategy to enhance the photo-cytotoxicity of 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin (p-OHTPP) ( Figure 18). Hyaluronic acid was also investigated by Jung et al [190], who synthesized novel types of nanophotosensitizers based on hyperbranched chlorin e6 (Ce6) conjugated with hyaluronic acid (HA) targeting CD44-receptors. The nano-photosensitizers showed higher intracellular accumulation of Ce6 and higher ROS generation and PDT efficacy than free Ce6.…”

Section: Formulation Techniquesmentioning

confidence: 99%

“…The formulations were also selective as confirmed by in vivo tumor xenograft, which demonstrated that the fluorescence intensity in the tumor tissues was much stronger than those in other organs. [189,190].…”

Section: Formulation Techniquesmentioning

confidence: 99%

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Porphyrin Derivative Nanoformulations for Therapy and Antiparasitic Agents

et al. 2020

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Porphyrins and analogous macrocycles exhibit interesting photochemical, catalytic, and luminescence properties demonstrating high potential in the treatment of several diseases. Among them can be highlighted the possibility of application in photodynamic therapy and antimicrobial/antiparasitic PDT, for example, of malaria parasite. However, the low efficiency generally associated with their low solubility in water and bioavailability have precluded biomedical applications. Nanotechnology can provide efficient strategies to enhance bioavailability and incorporate targeted delivery properties to conventional pharmaceuticals, enhancing the effectiveness and reducing the toxicity, thus improving the adhesion to the treatment. In this way, those limitations can be overcome by using two main strategies: (1) Incorporation of hydrophilic substituents into the macrocycle ring while controlling the interaction with biological systems and (2) by including them in nanocarriers and delivery nanosystems. This review will focus on antiparasitic drugs based on porphyrin derivatives developed according to these two strategies, considering their vast and increasing applications befitting the multiple roles of these compounds in nature.

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Hyaluronic Acid-Conjugated with Hyperbranched Chlorin e6 Using Disulfide Linkage and Its Nanophotosensitizer for Enhanced Photodynamic Therapy of Cancer Cells

Cited by 13 publications

References 54 publications

Evaluation of Hyaluronic Acid to Modulate Oral Squamous Cell Carcinoma Growth In Vitro

Evaluation of Hyaluronic Acid to Modulate Oral Squamous Cell Carcinoma Growth In Vitro

Application of phototherapeutic-based nanoparticles in colorectal cancer

Porphyrin Derivative Nanoformulations for Therapy and Antiparasitic Agents

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