Hyaluronate in healing of corneal alkali wound in the rabbit

Chung, Jang‐Hyun; Fagerholm, Per; Lindström, Bo

doi:10.1016/0014-4835(89)90039-0

Cited by 42 publications

(31 citation statements)

References 17 publications

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“…First, a suture-induced animal model could control the scope, depth and length of CNV more easily than an alkali burn animal model; also, alkali burns involve masses of immunopathology processes: neutrophilic granulocyte infiltration as well as the release of collagenase, which dissolves corneal collagen structures, even corneal stroma solution or perforation [29,30,31]. Second, physical methods can control the growth tendency of CNV more easily than chemical induction methods, which could help us to study which growth factors favor CNV.…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of the Effects of Recombinant Human Epidermal Growth Factor and Basic Fibroblast Growth Factor on Corneal Epithelial Wound Healing and Neovascularization in vivo and in vitro

Yan

Wang

et al. 2012

Ophthalmic Res

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Purpose: This study was undertaken to investigate the effects of recombinant human epidermal growth factor (rhEGF) and basic fibroblast growth factor (bFGF) on corneal wound healing and neovascularization (CNV). Methods: The positive effects of 10 ng/ml rhEGF and bFGF on the proliferation of corneal epithelial cells (SD-HCEC1s), rabbit keratocyte cells (RKCs) and human umbilical vein endothelial cells (HUVECs) as well as the effects on the migration capacity on HUVECs were observed. An animal central corneal wound and CNV model was established in rabbits. One eye of each group was chosen randomly for topical administration of rhEGF, bFGF or normal saline, and variability in the area of corneal epithelial wound healing and CNV was observed. Results: The optimal concentration of rhEGF and bFGF for the proliferation of corneal epithelial cells was 10 ng/ml. The promotive effect of 10 ng/ml rhEGF on the proliferation of RKCs and HUVECs was less than that of 10 ng/ml bFGF. In the animal experiment, the healing rate of the corneal epithelium in the rhEGF group was better than in the other groups on day 1. On day 3, the healing rates of the 3 groups were nearly equal. The CNV area in the rhEGF group was less than that of the bFGF group. Conclusions: rhEGF and bFGF both had promotive effects on corneal epithelial wound healing, but rhEGF had a weaker promotive effect on CNV than bFGF. With long-term application of growth factor drugs, rhEGF is suggested for lessening the growth of CNV.

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Section: Discussionmentioning

confidence: 99%

Comparative Study of the Effects of Recombinant Human Epidermal Growth Factor and Basic Fibroblast Growth Factor on Corneal Epithelial Wound Healing and Neovascularization in vivo and in vitro

Yan

Wang

et al. 2012

Ophthalmic Res

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“…Two were men and five were women, with an average age of 55 years (range: 42-65). The average duration of symptoms was 10 months (range: [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. All of the patients had globally restricted range of motion of the shoulder.…”

Section: Subjectsmentioning

confidence: 99%

“…6 HA plays an important role during repair of injured tissue, improving its healing with minimal formation of adhesions. [7][8][9] In controlled clinical trial, it has been found to exert beneficial effects in preventing adhesion. 10,11 Some investigators have studied the effect of HA in preventing adhesion of flexor tendons.…”

mentioning

confidence: 99%

Hyaluronan modulates cell proliferation and mRNA expression of adhesion‐related procollagens and cytokines in glenohumeral synovial/capsular fibroblasts in adhesive capsulitis

Nago

Mitsui

Gotoh

et al. 2010

Journal Orthopaedic Research

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There is a growing body of evidence supporting the use of hyaluronan (HA) in patients with adhesive capsulitis of the shoulder, although the mechanisms of the effect have not yet been clarified. This in vitro study examined the effects of HA on glenohumeral synovial/ capsular fibroblasts (GSCFs) from patients with adhesive capsulitis of the shoulder. The study subjects were seven patients with primary or secondary adhesive capsulitis of the shoulder (average age: 55 years; range: 42-65). Synovial/capsular specimens were obtained from the rotator interval of each patient during arthroscopy. Part of the tissue specimen was used for histological analysis. The remainder of the tissue was prepared for cell culture. Various concentrations of HA (0.0-4.0 mg/mL) were added to the monolayer-cultured GSCFs from these patients. Histological analysis consistently demonstrated chronic nonspecific inflammation with synovial hyperplasia, proliferation of vessels and fibroblasts, and increased amount of extracellular matrix. Treatment with HA at various concentrations significantly and dose-dependently inhibited cell proliferation and decreased the expression levels of mRNA for adhesion-related procollagens and cytokines. Pretreatment with OS/37 did not reverse the inhibitory effect of HA. These results suggest that HA modulates cell proliferation and expression of the mRNA of adhesion-related procollagens and cytokines in GSCFs, preventing the progression of adhesion formation in patients with adhesive capsulitis of the shoulder. ß

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“…The high molecular weight hyaluronan (Healon) acts as a protective factor for the ocular tissues, especially the endothelium in anterior segment surgery (Holmberg & Philipson 1984a, 1984b. It also has a positive effect on corneal epithelial healing and corneal wound healing (Chung et al 1989).…”

mentioning

confidence: 99%