Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition.

Abstract

SummaryA cell adhesion model was previously used to select a series of monoclonal antibodies (mAbs), which were subsequently found to recognize CD44/Pgp-1 . Interest in these reagents increased with the finding that they totally inhibited production of lymphoid or myeloid cells in long-term bone marrow cultures. Further investigation has now revealed that hyaluronate is a potential ligand for CD44 and that hyaluronate recognition accounts for the adhesion between B lineage hybridoma and stromal cells . The hybridoma cells adhered to hyaluronate-coated plastic wells as well as to monolayers of stromal cells. The adhesion in both cases was inhibited by treatment with hyaluronidases, and did not require divalent cations . Addition of exogenous hyaluronate also diminished binding of lymphoid cells to stromal cells. One of several mAbs to Pgp-1/CD44 was particularly effective at blocking these interactions. Since hyaluronate and Pgp-1/CD44 were present on both cell types, experiments were done to determine the cellular location ofinteracting molecules required for the adhesion process . Treatment oflymphoid cells with an anti-Pgp-1/CD44 antibody was more inhibitory than antibody treatment ofthe stromal cells. Conversely, hyaluronidase treatment of stromal cells reduced subsequent binding more than treatment of the lymphoid cells . Adhesive interactions that involve hyaluronate and CD44 could contribute to a number of cell recognition processes, including ones required for normal lympho-hemopoiesis .

Regulation of cellular adhesion molecule expression in murine oocytes, peri-implantation and post-implantation embryos

“…It is the principal cell surface receptor for hyaluronate [35], [36] which is widely distributed in extracellular matrices and present within the extracellular matrix of the cumulus cell-oocyte complex [37]. While successful implantation and embryonic development can occur in its absence [38], notwithstanding, it is implicated in a number of biological phenomena, including cell-cell adhesion, migration, embryonic development and pathogenesis [39], [40].…”

Hyaluronan: fundamental principles and applications in cancer

“…CD44 exhibits affinity to HYA on lymphocytes and could account for adhesion between lymphocytes of B lineage and stromal cells [24,25]. It mediates the HYA-binding of human myeloid KG1 and KG1 A cells [26].…”

“…As noted above, this binding site was later defined as CD44, which is responsible for the adhesion of HYA to endothelial cells [22] and human myeloid cell lines [26], and for the adhesion of B-lineage lymphocytes to stroma cells [25]. CD44 can be distinguished in two forms, one epithelial and the other haemopoietic, with different adhesion potentials for HYA-bearing cells [27,28].…”

Culture system for extensive production of CD19+IgM+ cells by human cord blood CD34+ progenitors

“…Comparison of cell-cell contact and cell-cell noncontact culture systems for B cell development It has been reported that cell-cell interactions between stroma and progenitor cells play important roles in early Blymphopoiesis in both murine 11,[27][28][29][30][31] and human [32][33][34][35] systems. Therefore, to elucidate whether cell-cell interactions between MS-5 cells and progenitor cells are required for the generation of CD19 + cells in our culture, CD34 + cells were cultured with or without the 0.45 m microporous filter on an MS-5 cell layer.…”