Hyaluronan Used for Intradermal Injections Is Incorporated into the Pericellular Matrix and Promotes Proliferation in Human Skin Fibroblasts in vitro

Röck, Katharina; Fischer, Kerstin; Fischer, Jens W.

doi:10.1159/000318905

Cited by 24 publications

(15 citation statements)

References 87 publications

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“…1). This is particularly relevant in dynamic tissue environments, where sugar cell coats are involved in the regulation of a wide range of processes that involve cell rearrangements including cell migration (3)(4)(5), division (6), synaptogenesis (7), morphogenesis (8,9), cancer progression and metastasis (10)(11)(12). The aim of this study is to investigate the physical role that the hyaluronan sugar coat plays in mediating cell surface access.…”

Section: Introductionmentioning

confidence: 99%

Cell Surface Access Is Modulated by Tethered Bottlebrush Proteoglycans

Chang

McLane

Fogg

et al. 2016

Biophysical Journal

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The hyaluronan-rich pericellular matrix (PCM) plays physical and chemical roles in biological processes ranging from brain plasticity, to adhesion-dependent phenomena such as cell migration, to the onset of cancer. This study investigates how the spatial distribution of the large negatively charged bottlebrush proteoglycan, aggrecan, impacts PCM morphology and cell surface access. The highly localized pericellular milieu limits transport of nanoparticles in a size-dependent fashion and sequesters positively charged molecules on the highly sulfated side chains of aggrecan. Both rat chondrocyte and human mesenchymal stem cell PCMs possess many unused binding sites for aggrecan, showing a 2.5x increase in PCM thickness from ∼7 to ∼18 μm when provided exogenous aggrecan. Yet, full extension of the PCM occurs well below aggrecan saturation. Hence, cells equipped with hyaluronan-rich PCM can in principle manipulate surface accessibility or sequestration of molecules by tuning the bottlebrush proteoglycan content to alter PCM porosity and the number of electrostatic binding sites.

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Section: Introductionmentioning

confidence: 99%

Cell Surface Access Is Modulated by Tethered Bottlebrush Proteoglycans

Chang

McLane

Fogg

et al. 2016

Biophysical Journal

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“…According to several investigations, a monophasic, non-cross-linked HA with uniform particle size results in uniform spreading and defect augmentation compared to biphasic HA product [17]. On the other hand, Röck et al [18] reported excessive fibroblast proliferation induced by non-cross-linked HA formulation, and Wang et al [19] reported the de novo collagen production induced by cross-linked HA in photo-damaged human skin. These findings indicate that HA injection applied to humans might be useful for stimulating collagen production, which could achieve a longer augmentation effect.…”

Section: Discussionmentioning

confidence: 99%

Efficiency and durability of hyaluronic acid of different particle sizes as an injectable material for VF augmentation

Kim¹,

Choi

Park

et al. 2015

Acta Oto-Laryngologica

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After 6 and 10 weeks, the injected site was evaluated endoscopically, histologically, radiologically, and functionally. None of the 24 rabbits showed any signs of respiratory distress. Computed tomography (CT) images and endoscopic evaluation revealed sufficient augmented volume of the injected VF in all treated groups 6 weeks after the injection. Histological data at week 10 showed that unequal particle-sized HA did not migrate from its original injection site, while other HAs migrated to the periphery of the arytenoid cartilage. Videokymographic analysis showed more favorable vibrations of unequal particle-sized HA injected VF mucosa 10 weeks post-injection, compared to the other treatment groups.

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“…This indicates that soluble HA facilitates assemble of a supramolecular pericellular structure [ 48 ] . Whether cross-linked HA has this property has not been established, nor whether injected stabilized HA can perform such functions.…”

Section: Applications Of Hyaluronan From Outside Skinmentioning

confidence: 92%

Hyaluronan Inside and Outside of Skin

Wahby

DiCaprio

Stern

2012

Treatment of Dry Skin Syndrome

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The glycosaminoglycan hyaluronic acid (also called hyaluronan or hyaluronate and abbreviated as HA) is a major component of the extracellular matrix (ECM) of skin and plays an important role in the metabolism of both the epidermis and dermis. Hyaluronan is responsible for hydration, nutrient exchange, and protects against free radical damage via a multitude of signaling pathways. It is also involved in basic biologic processes such as cell differentiation and motility. An overview is provided here that provides recent information, bringing up-to-date advances in matrix biology relevant for dermatology and skin care, with a particular emphasis on skin moisture. HyaluronanHyaluronan is a highly anionic molecule. At the body's pH, it is one of the most highly charged molecules in biology, which provides HA with some of its unique qualities. A massive cloud of water surrounds the molecule in an attempt to neutralize the charge. It is this particular quality which provides the hydrating functions of HA, with the simultaneous ability to expand tissues and to open spaces for cell movement.Extrinsic aging in the human skin, compared with photo-protected skin is associated with alterations in the expression of HA and its metabolizing enzymes, both the hyaluronidases and the HA synthase complex of enzymes [ 58 ] , as described below. It is clear that the "dried" appearance of aging skin is intimately associated with changes in apparent levels and types of HA deposition, dependent on changes in controls of its underlying metabolism.Hyaluronan is involved in multiple aspects of skin biology, responsible not only for skin hydration but also for nutrient exchange, tissue homeostasis, repair processes, protection against free radical damage, cell differentiation, and cell motility. Native and formulated preparations of HA, applied exogenously, help skin regain elasticity, turgor, as well as moisture. Understanding HA metabolism may provide clues for reversing some of the processes that lead to skin aging, loss of moisture, and wrinkling. Hyaluronan in SkinThere are numerous reports of decreased amounts of HA in aging skin. These observations are based on histochemical stains, such as Alcian blue, and affi nity histochemistry with the

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Hyaluronan Used for Intradermal Injections Is Incorporated into the Pericellular Matrix and Promotes Proliferation in Human Skin Fibroblasts in vitro

Cited by 24 publications

References 87 publications

Cell Surface Access Is Modulated by Tethered Bottlebrush Proteoglycans

Cell Surface Access Is Modulated by Tethered Bottlebrush Proteoglycans

Efficiency and durability of hyaluronic acid of different particle sizes as an injectable material for VF augmentation

Hyaluronan Inside and Outside of Skin

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