Hyaluronan stimulates corneal epithelial migration

Nishida, Teruo; Nakamura, Masatsugu; Mishima, Hiroshi; Otori, Toshifumi

doi:10.1016/0014-4835(91)90110-z

Cited by 163 publications

(101 citation statements)

References 20 publications

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“…By producing extracellular matrix, fibroblasts are known to pilot cell differentiation, migration and adhesion, with pro-healing and anti-inflammatory effects [18,53,54], therefore the protection against UV-B or Fenton reaction-induced ROS, exerted by both compounds on SIRC, may acquire a considerable importance. Indeed SIRC, being basically fibroblasts (keratocytes), possess a low (or absent) expression of some epithelial enzymes suitable to inhibit the ROS attack [48,55,56].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure

Ciuffi

Pisanello

Pagliai

et al. 2003

Journal of Photochemistry and Photobiology B: Biology

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Several corneal pathologies are characterized by the presence of reactive oxygen species (ROS); therefore, we evaluated the protection afforded by pirenoxine and melatonin to corneal cell culture and whole rabbit cornea from ultraviolet exposure and other oxidant systems.Rabbit cornea cell (SIRC) plates and whole corneas were exposed to UV-B (80 or 800 mJ/cm 2 ) or incubated with fMLPstimulated autologous macrophages, in the presence or absence of pirenoxine or melatonin (10 À5 M). The protective activity of compounds was assessed by measuring superoxide anion formation, inhibition of oxidation and mitochondrial viability. Moreover the ex vivo protective effect of pirenoxine and melatonin was verified in the whole cornea submitted to UV-B exposure in vitro.Our experimental data demonstrate that pirenoxine and melatonin were able to inhibit the superoxide formation and oxidative effect in cell culture and whole rabbit corneas submitted to UV-B exposure or to incubation with fMLP-stimulated autologous macrophages.Mitochondrial viability was restored in epithelial cells of rabbit cornea but not in SIRCs. Moreover, both compounds are also able to increase ex vivo epithelial corneal cell defences against the in vitro UV-B induced lipid peroxidation.

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Section: Discussionmentioning

confidence: 99%

Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure

Ciuffi

Pisanello

Pagliai

et al. 2003

Journal of Photochemistry and Photobiology B: Biology

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“…Numerous cellular processes including cell migration (Chen et al, 1989), adhesion (Klein et al, 1996), and proliferation (Mast et al, 1993;Wiig et al, 1996) are influenced by HA. Thus, HA has an important role in such processes as morphogenesis (Toole, 1997), wound healing (Nishida et al, 1991), inflammation (Weigel et al, 1988), and immune cell trafficking (Mohamadzadeh et al, 1998), as well as in many aspects of tumor biology (Delpech et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Layilin, a Novel Integral Membrane Protein, Is a Hyaluronan Receptor

Bono

Rubin

Higgins

et al. 2001

MBoC

131

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The actin cytoskeleton plays a significant role in changes of cell shape and motility, and interactions between the actin filaments and the cell membrane are crucial for a variety of cellular processes. Several adaptor proteins, including talin, maintain the cytoskeleton-membrane linkage by binding to integral membrane proteins and to the cytoskeleton. Layilin, a recently characterized transmembrane protein with homology to C-type lectins, is a membrane-binding site for talin in peripheral ruffles of spreading cells. To facilitate studies of layilin's function, we have generated a layilin-Fc fusion protein comprising the extracellular part of layilin joined to human immunoglobulin G heavy chain and used this chimera to identify layilin ligands. Here, we demonstrate that layilin-Fc fusion protein binds to hyaluronan immobilized to Sepharose. Microtiter platebinding assays, coprecipitation experiments, and staining of sections predigested with different glycosaminoglycan-degrading enzymes and cell adhesion assays all revealed that layilin binds specifically to hyaluronan but not to other tested glycosaminoglycans. Layilin's ability to bind hyaluronan, a ubiquitous extracellular matrix component, reveals an interesting parallel between layilin and CD44, because both can bind to cytoskeleton-membrane linker proteins through their cytoplasmic domains and to hyaluronan through their extracellular domains. This parallelism suggests a role for layilin in cell adhesion and motility. INTRODUCTIONCell shape and movement form the basis for several biological phenomena such as morphogenesis, invasion, and metastasis. The actin cytoskeleton is a major determinant of changes in cell shape, and interactions between actin filaments and the cell membrane are essential for cell adhesion, spreading, and migration, as well as for signal transduction (Hall, 1998;Schoenwaelder and Burridge, 1999). Several molecules have been recognized as linkers between the actin cytoskeleton and the cell membrane. For example, members of the band 4.1/ERM superfamily (band 4.1, talin, ezrin, radixin, moesin, and merlin, the product of the neurofibromatosis type 2 tumor suppressor gene) are characterized by the presence of a conserved N-terminal membrane-binding domain, which is able to bind to the cytoplasmic region of several transmembrane receptors, including CD44, CD43, ICAM-2, and ICAM-3 (Sainio et al., 1997;Heiska et al., 1998;Yonemura et al., 1998). The C-terminal regions of ERM proteins are thought to bind to actin filaments, thereby linking these transmembrane receptors to the cytoskeleton (reviewed by Mangeat et al., 1999).Layilin is a recently cloned ϳ55-kDa membrane-binding partner for talin (Borowsky and Hynes, 1998) that is widely expressed in different cell types and tissue extracts. It is found in peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells induced to migrate in in vitro wounding experiments. Layilin colocalizes with talin in ruffles and binds to talin's ϳ50-kDa head domain (amino acids 280 -435)...

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“…KGF, a paracrine hormone secreted by stromal cells, has been shown to enhance outgrowth in rabbit limbal explant culture on human amniotic membrane [60] . While the inflammatory cytokine interleukin-6 [61] , fibronectin [62] , and hyaluronan [63] , all of which are highly up-regulated upon injury, have been shown to play a role in drawing rabbit limbal cells towards the wound.…”

Section: Biochemical Characteristicsmentioning

confidence: 99%

Limbal stem cells: Central concepts of corneal epithelial homeostasis

Yoon¹

2014

WJSC

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A strong cohort of evidence exists that supports the localisation of corneal stem cells at the limbus. The distinguishing characteristics of limbal cells as stem cells include slow cycling properties, high proliferative potential when required, clonogenicity, absence of differentiation marker expression coupled with positive expression of progenitor markers, multipotency, centripetal migration, requirement for a distinct niche environment and the ability of transplanted limbal cells to regenerate the entire corneal epithelium. The existence of limbal stem cells supports the prevailing theory of corneal homeostasis, known as the XYZ hypothesis where X represents proliferation and stratification of limbal basal cells, Y centripetal migration of basal cells and Z desquamation of superficial cells. To maintain the mass of cornea, the sum of X and Y must equal Z and very elegant cell tracking experiments provide strong evidence in support of this theory. However, several recent studies have suggested the existence of oligopotent stem cells capable of corneal maintenance outside of the limbus. This review presents a summary of data which led to the current concepts of corneal epithelial homeostasis and discusses areas of controversy surrounding the existence of a secondary stem cell reservoir on the corneal surface

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Hyaluronan stimulates corneal epithelial migration

Cited by 163 publications

References 20 publications

Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure

Antioxidant protection in cultured corneal cells and whole corneas submitted to UV-B exposure

Layilin, a Novel Integral Membrane Protein, Is a Hyaluronan Receptor

Limbal stem cells: Central concepts of corneal epithelial homeostasis

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