Hyaluronan–Phospholipid Interactions

Pasquali‐Ronchetti, I.; Quaglino, Daniela; Mori, G.; Bacchelli, Barbara; Ghosh, Peter

doi:10.1006/jsbi.1997.3908

Cited by 108 publications

(99 citation statements)

References 25 publications

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“…HA forms a three-dimensional macromolecular mesh in water that may also act to separate inactivating substances from surfactant by acting as a size-discriminating filter (31). Phospholipids in the presence of HA tend to form lipid aggregates (17). HA may also stabilize surfactant phospholipids at the air-water interface and/or increase surface adsorption of subphase lipids.…”

Section: Discussionmentioning

confidence: 99%

“…However, HA (like PEG and dextran) shares the ability to bind water but has much greater binding capacity. In addition, HA can interact with phospholipids to form various complexes that depend on the molecular weight of HA (17). HA and other glycosaminoglycans are secreted by alveolar epithelial cells (18,19), and early papers already speculated that complex carbohydrates may interact in important ways with pulmonary surfactant under normal conditions in the alveoli (20 -22).…”

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Hyaluronan Decreases Surfactant Inactivation In Vitro

Goerke

Clements

et al. 2005

Pediatr Res

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Hyaluronan (HA) is an anionic polymer and a constituent of alveolar fluid that can bind proteins, phospholipids, and water. Previous studies have established that nonionic polymers improve the surface activity of pulmonary surfactants by decreasing inactivation of surfactant. In this work, we investigate whether HA can also have beneficial effects when added to surfactants. We used a modified pulsating bubble surfactometer to measure mixtures of several commercially available pulmonary surfactants or native calf surfactant with and without serum inactivation. Surface properties such as equilibrium surface tension, minimum and maximum surface tensions on compression and expansion of a surface film, and degree of surface area reduction required to reach a surface tension of 10 mN/m were measured. In the presence of serum, addition of HA dramatically improved the surface activities of all four surfactants and in some cases in the absence of serum as well. These results indicate that HA reduces inactivation of surfactants caused by serum and add evidence that endogenous HAs may interact with alveolar surfactant under normal and abnormal conditions. Over the past decade, pulmonary surfactant replacement has revolutionized the therapy of respiratory distress syndrome of premature infants (1-3). However, effects of surfactant therapy are less dramatic when used to treat lung diseases associated with acute lung injury and acute respiratory distress syndrome (4 -7). The less successful clinical response in these diseases may be due in part to surfactant inactivation caused by leakage of plasma and inflammatory products into the alveoli. In the presence of inactivating substances, surface activity of alveolar surfactant is adversely affected, worsening overall lung function (8,9).Variation in susceptibility to inactivation exists among therapeutic surfactants. Many studies have reported that surfactantassociated proteins help resist inactivation caused by a variety of factors (6). We and others have found that the addition of nonionic polymers [principally polyethylene glycol (PEG), or dextran] further reduces surfactant inactivation by serum, meconium, or other substances in vitro (10 -12). In vivo studies also demonstrate that the addition of nonionic polymers to therapeutic surfactants improves pulmonary function after lung injury caused by meconium, albumin, hydrochloric acid, milk acid, or endotoxin (11,(13)(14)(15)(16).The successful experiments with nonionic polymers led us to consider using ionic polymers. In this study, we investigated whether hyaluronan (HA) has effects on pulmonary surfactants that are similar to those described for dextran and PEG. HA is a nonsulfated, polymeric glycosaminoglycan (mucopolysaccharide) of N-acetyl-glucosamine linked ␤-1, 4 to glucuronic acid, which is linked ␤-1, 3 to N-acetylglucosamine, etc. Unlike PEG or dextran, HA, an anionic polymer, is a natural, ubiquitous substance in the body. However, HA (like PEG and dextran) shares the ability to bind water but has much greater bind...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Hyaluronan Decreases Surfactant Inactivation In Vitro

Goerke

Clements

et al. 2005

Pediatr Res

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“…T he Haplex was pre pared by film dispersion and sonication method [7,8] . The mixture of HA and PL was obtained by grinding in mortar.…”

Section: Haplex Preparationmentioning

confidence: 99%

Oral absorption of hyaluronic acid and phospholipids complexes in rats

Huang¹

2007

WJG

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AIM:To prepare a complex of hyaluronic acid (HA) and phospholipids (PL), and study the improvement effect of PL on the oral absorption of HA. METHODS:The complex of HA-PL (named Haplex) was prepared by film dispersion and sonication method, its physico-chemical properties were identified by infrared spectra and differential scanning calorimetry (DSC). The oral absorption of Haplex was studied. Thirty-two healthy rats were divided into 4 groups randomly: (1) a normal saline (NS) control group; (2) an HA group; (3) a mixture group and (4) a Haplex group. After intragastric administration, the concentration of HA in serum was determined. RESULTS:The physico-chemical properties of Haplex were different from HA or PL or their mixture. After Haplex was administered to rats orally, the serum concentration of HA was increased when compared with the mixture or HA control groups from 4 h to 10 h (P < 0.05). The ∆AUC0-12 h of Haplex was also greater than that of the other three groups (P < 0.05). CONCLUSION:The method of film dispersion and sonication can prepare HA and PL complex, and PL can enhance the oral absorption of exogenous HA.© 2007 The WJG Press. All rights reserved.Key words: Hyaluronic acid; Phospholipids; Complex; Oral absorption; Infrared spectrum; Thermochemistry H u a n g S L , L i n g P X , Z h a n g T M . O ra l a b s o r p t i o n o f hyaluronic acid and phospholipids complexes in rat. World J Gastroenterol 2007; 13(6): 945-949

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“…Thus, RHAMM may be an actual intracellular binding partner for the hyaluronan here. Hyaluronan interacts with phospholipid (Pasqualli-Ronchetti et al 1997) and is extruded through the membrane during biosynthesis. Therefore, it is not inconceivable that hyaluronan within vesicles or other membranous compartment has the capacity to interact with RHAMM.…”

Section: Hyaluronan Nuclear Architecture Microtubule Function and Mmentioning

confidence: 99%

Intracellular Hyaluronan in Arterial Smooth Muscle Cells: Association with Microtubules, RHAMM, and the Mitotic Spindle

Evanko¹,

Parks

Wight³

2004

J Histochem Cytochem.

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S U M M A R YAlthough considered a pericellular matrix component, hyaluronan was recently localized in the cytoplasm and nucleus of proliferating cells, supporting earlier reports that hyaluronan was present in locations such as the nucleus, rough endoplasmic reticulum, and caveolae. This suggests that it can play roles both inside and outside the cell. Hyaluronan metabolism is coupled to mitosis and cell motility, but it is not clear if intracellular hyaluronan associates with cytoskeletal elements or plays a structural role. Here we report the distribution of intracellular hyaluronan, microtubules, and RHAMM in arterial smooth muscle cells in vitro. The general distribution of intracellular hyaluronan more closely resembled microtubule staining rather than actin filaments. Hyaluronan was abundant in the perinuclear microtubule-rich areas and was present in lysosomes, other vesicular structures, and the nucleolus. Partially fragmented fluorescein-hyaluronan was preferentially translocated to the perinuclear area compared with high-molecular-weight hyaluronan. In the mitotic spindle, hyaluronan colocalized with tubulin and with the hyaladherin RHAMM, a cell surface receptor and microtubule-associated protein that interacts with dynein and maintains spindle pole stability. Internalized fluorescein-hyaluronan was also seen at the spindle. Following telophase, an abundance of hyaluronan near the midbody microtubules at the cleavage furrow was also noted. In permeabilized cells, fluorescein-hyaluronan bound to RHAMM-associated microtubules. These findings suggest novel functions for hyaluronan in cellular physiology.

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Hyaluronan–Phospholipid Interactions

Cited by 108 publications

References 25 publications

Hyaluronan Decreases Surfactant Inactivation In Vitro

Hyaluronan Decreases Surfactant Inactivation In Vitro

Oral absorption of hyaluronic acid and phospholipids complexes in rats

Intracellular Hyaluronan in Arterial Smooth Muscle Cells: Association with Microtubules, RHAMM, and the Mitotic Spindle

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