Hyaluronan in the Extracellular Matrix of Hematological and Solid Tumors. Its Biological Effects

Icardi, Antonella; Lompardía, Silvina; Papademetrio, Daniela L.; Rosales, Paolo; Díaz, Mariángeles; Pibuel, Matías; Alaniz, Laura; Álvarez, Élida

doi:10.1007/978-3-030-99708-3_7

Cited by 2 publications

(2 citation statements)

References 219 publications

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“…[ 9–11 ] HA is known to impact the hydration, lubrication, and physical properties of tissues. [ 12,13 ] It consists of a repeating disaccharide chain composed of [3)‐β‐d‐ N ‐acetylglucosamine (GlcNAc)‐β(1,4)‐d‐glucuronic acid (GlcA)‐β(1)]. [ 14 ] HA contributes to the structural integrity of the ECM and triggers various signaling pathways, regulating cellular processes through its interaction with principal receptors, CD44 and RHAMM.…”

Section: Introductionmentioning

confidence: 99%

Matrix‐Bound Hyaluronan Molecular Weight as a Regulator of Dendritic Cell Immune Potency

Quartey,

Sapudom,

ElGindi

et al. 2023

Adv Healthcare Materials

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Hyaluronic acid (HA) is a glycosaminoglycan in the extracellular matrix with immunoregulatory properties depending on its molecular weight (MW). However, the impact of matrix‐bound HA on dendritic cells (DCs) remains unclear due to varying distribution of HA MW under different physiological conditions. To investigate DCs in defined biosystems, 3D collagen matrices modified with HA of specific MW with similar microstructure and HA levels are used. It is found that HA MW influences cytokine binding to matrix, suggesting modulation of cytokine availability by the different HA MWs . These studies on DC immune potency reveal that low MW HA (8–15 kDa) enhances immature DC differentiation and antigen uptake, while medium (MMW‐HA; 500–750 kDa) and high MW HA (HMW‐HA; 1250–1500 kDa) increase cytokine secretion in mature DCs. The effect on DC phenotype and cytokine secretion by different MWs of HA is independent of CD44. However, blocking the CD44 receptor reveals its potential role in regulating acute inflammation through increased secretion of CCL2, CXCL8, and IL‐6. Additionally, MMW‐ and HMW‐HA matrices reduce migratory capacity of DCs, dependent on CD44. Overall, these findings provide insights into MW‐dependent effects of matrix‐bound HA on DCs, opening avenues for the design of DC‐modulating materials to enhance DC‐based therapy.

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Section: Introductionmentioning

confidence: 99%

Matrix‐Bound Hyaluronan Molecular Weight as a Regulator of Dendritic Cell Immune Potency

Quartey,

Sapudom,

ElGindi

et al. 2023

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…One crucial ECM component, hyaluronan (HA), is a glycosaminoglycan that exhibits a high turnover rate and possesses immunomodulatory properties [9][10][11]. HA is known to impact hydration, lubrication, and physical properties of tissues [12,13]. It consists of a repeating disaccharide chain composed of [3)-β-d-N-acetylglucosamine (GlcNAc)-β(1,4)-d-glucuronic acid (GlcA)-β(1)] [14].…”

Section: Introductionmentioning

confidence: 99%

Tissue-bound hyaluronan molecular weight as a regulator of dendritic cell immune potency

Quartey

Sapudom

ElGindi

et al. 2023

Preprint

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Hyaluronic acid (HA) is a major glycosaminoglycan found in the extracellular matrix (ECM) and exhibits immunoregulatory properties depending on its molecular weight (MW). However, the impact of tissue bound HA on dendritic cell (DC) functions is not well understood due to the varying distribution of HA MW under different physiological and pathological conditions. To investigate DCs in defined biosystems, we used three-dimensional (3D) collagen matrices modified with HA of specific MW, while maintaining similar microstructure and HA levels. Using these matrices, we examined the influence of HA on cytokine binding and observed distinct properties depending on the presence and MW of HA, suggesting modulation of cytokine availability by the different MW of HA. Our studies on DC immune potency revealed that low molecular weight HA (LMW-HA; 8-15 kDa) enhances immature DC (iDC) differentiation and antigen uptake, while medium (MMW-HA; 500-750 kDa) and high molecular weight HA (HMW-HA; 1250-1500 kDa) increase cytokine secretion in matured DCs (mDCs). Interestingly, the modulation of DCs surface marker expression and cytokine secretion by different MW of HA appeared to be independent of CD44. However, we found that cytokine secretion of DCs was dependent on the CD44 receptor regardless of the presence or absence of HA in the matrix. Additionally, we observed reduced migratory capacity of iDCs and mDCs when cultured on MMW- and HMW-HA matrices, and this effect was dependent on CD44. In summary, our findings provide new insights into the MW-dependent effects of tissue-bound HA on DCs, opening avenues for the design of DC-modulating materials to enhance DC-based therapy.

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Hyaluronan in the Extracellular Matrix of Hematological and Solid Tumors. Its Biological Effects

Cited by 2 publications

References 219 publications

Matrix‐Bound Hyaluronan Molecular Weight as a Regulator of Dendritic Cell Immune Potency

Matrix‐Bound Hyaluronan Molecular Weight as a Regulator of Dendritic Cell Immune Potency

Tissue-bound hyaluronan molecular weight as a regulator of dendritic cell immune potency

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