Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection

Dokoshi, Tatsuya; Zhang, Ling-juan; Li, Fengwu; Nakatsuji, Teruaki; Butcher, A.; Yoshida, Hiroyuki; Shimoda, Masayuki; Okada, Yasunori; Gallo, Richard L.

doi:10.1016/j.celrep.2019.12.001

Cited by 30 publications

(21 citation statements)

References 42 publications

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“…A recent study in mice revealed that Cemip expression is induced during Staphylococcus aureus dermis infection ( Dokoshi et al., 2020 ). Taking advantage of mice lacking Cemip gene expression, the researchers demonstrated its central role in hyaluronan digestion during dermis infection and in regulating antimicrobial activity.…”

Section: Discussionmentioning

confidence: 99%

NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses

et al. 2021

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Summary Mesenchymal stromal cells (MSCs) combined with calcineurin-nuclear factor of activated T cell (CN-NFAT) inhibitors are being tested as a treatment for graft-versus-host disease (GvHD). The immunosuppressive properties of MSCs seem beneficial; however, their response during fungal infection, which is an important cause of mortality in patients with GvHD , is unknown. We report that MSCs phagocytose the fungal component zymosan, resulting in phosphorylation of spleen tyrosine kinase (Syk), increase in cytosolic calcium levels, and ultimately, increase in NFAT1 nuclear translocation. RNA sequencing analysis of zymosan-treated MSCs showed that CN-NFAT inhibition affects extracellular matrix (ECM) genes but not cytokine expression that is under the control of the NF-κB pathway. When coculturing MSCs or decellularized MSC-ECM with human peripheral blood mononuclear cells (PBMCs), selective NFAT inhibition in MSCs decreased cytokine expression by PBMCs. These findings reveal a dual mechanism underlying the MSC response to zymosan: while NF-κB directly controls inflammatory cytokine expression, NFAT impacts immune-cell functions by regulating ECM remodeling.

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Section: Discussionmentioning

confidence: 99%

NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses

et al. 2021

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“… 8 , 9 Bacteria living in biofilms can resist harsh living conditions and aggravate chronic infections compared with planktonic bacteria. 10 It is well known that antibiotic can effectively suppress the growth of bacteria, but it is challenging to eradicate all the bacteria living under the biofilms. 11 The resistance to traditional antibiotics makes biofilm-related infections more difficult to be treated compared to planktonic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

Xu¹,

Li²,

Wang³

et al. 2021

IJN

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Background:The biofilms could protect bacteria from antibiotics and promote the production of drug-resistant strains, making the bacteria more difficult to be eradicated. Thus, we developed an AMP@PDA@AgNPs nanocomposite, which is formed by modifying silver nanoparticles (AgNPs) with antimicrobial peptides (AMP) modified nanocomposite to destroy biofilm in this study. Methods: The AMP@PDA@AgNPs nanocomposite was prepared with polymerization method and characterized by using ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), Fourier transform-infrared spectroscopy (FT-IR), and transmission electron microscope (TEM). The antibacterial effects of the nanocomposite were investigated by using agar diffusion method and minimum inhibitory concentration (MIC) test. The quantitative analysis of the biofilm formation by the nanocomposite was conducted using crystal violet staining and confocal laser scanning microscope (CLSM). Results: The DLS and TEM analysis showed it was a spherical nanocomposite with 200 nm size and well dispersed . The results of UV-vis and FT-IR confirmed the presence of AMP and AgNPs. The nanocomposite had an excellent biocompatibility at 100 μg/mL. And the AMP@PDA@AgNPs nanocomposite showed superior antimicrobial activity against both Gram-negative (E. coli, P. aeruginosa) and Gram-positive (S. aureus) bacteria than AgNPs or AMP. Importantly, the mRNA expression of biofilm-related genes were decreased under the action of the nanocomposites. Conclusion: An AMP@PDA@AgNPs nanocomposite with good biocompatibility was successfully prepared. The nanocomposite could destruct bacterial biofilms by inhibiting the expression of biofilm-related genes. The synergistic strategy of AMPs and AgNPs could provide a new perspective for the treatment of bacterial infection.

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“…We found that the abundances of resting NK cells, M0 macrophages, and M2 macrophages were substantially different between HRG and LRG. In addition, many international studies have shown that BMP2, BMPR2, GREM1, HGF, MCAM, TGFBR1, and other genes might promote distant metastasis and the local progression of BC [29] , [30] , [31] , [32] , [33] , [34] , [35] .…”

Section: Discussionmentioning

confidence: 99%

Analysis of genomics and immune infiltration patterns of epithelial-mesenchymal transition related to metastatic breast cancer to bone

Liu

et al. 2021

Translational Oncology

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Highlights Differential expression analysis showed a total of 304 differentially expressed genes, which were mainly related to proteoglycans in cancer, the PI3K/Akt signaling pathway, and the TGF-beta signaling pathway. The survival-related linear risk assessment model consisting of eight genes (FERMT2, ITGA5, ITGB1, MCAM, CEMIP, HGF, TGFBR1, F2RL2) was constructed. The survival rates of high-risk patients were significantly lower than that of the low-risk group, and the 3-, 5-, and 10-year AUCs were satisfactory. BMP2, BMPR2 , and GREM1 were differentially expressed in both data sets of breast cancer bone metastasis. In GSE20685 and GSE45255, significant differences in immune infiltration patterns were found between high- and low-risk groups. BMP-2 may regulate the immune infiltration process in breast cancer tissues through the PI3K/Akt signaling pathway.

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Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection

Cited by 30 publications

References 42 publications

NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses

NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses

Enhanced Antibacterial and Anti-Biofilm Activities of Antimicrobial Peptides Modified Silver Nanoparticles

Analysis of genomics and immune infiltration patterns of epithelial-mesenchymal transition related to metastatic breast cancer to bone

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