Hyaluronan-coated nanoparticles: The influence of the molecular weight on CD44-hyaluronan interactions and on the immune response

Mizrahy, Shoshy; Raz, Sabina Rebe; Hasgaard, Martin; Liu, Hong; Soffer-Tsur, Neta; Cohen, Keren; Dvash, Ram; Landsman-Milo, Dalit; Bremer, M.G.E.G.; Moghimi, S. Moein; Peer, Dan

doi:10.1016/j.jconrel.2011.06.031

Cited by 213 publications

(200 citation statements)

References 52 publications

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“…This caveolae mediated endocytosis is consistent with the internalization pathway of DMRIE:cholesterol (1:1) lipoplexes [36], but not with DOTAP lipoplexes that are internalized in A549 mainly by clathrin-mediated endocytosis [35]. Moreover our results are in accordance with others that show the ability of HA-bearing liposomes to interact with cells expressing CD44 [25] and [29]. The DE-based, HA-bearing lipoplexes based on HA-DOPE, which display an interesting stability in serum-containing media, are thus promising for further in vivo evaluation using an anticancer therapeutic gene.…”

Section: Discussionsupporting

confidence: 92%

“…This also confirms the crucial role of the receptor in the endocytosis mediated process, as shown previously by us and others [25] and [29].…”

Section: Discussionsupporting

confidence: 91%

“…reporting that unilamellar liposomes of 100 nm, containing 1500 kDa HA and composed of PC:Chol:DPPE slightly enhance complement activation [25]. This slight increase of complement activation in the presence of HA-DOPE could be due to polysaccharidic nature of HA, even if it is the lowest activator of glycosaminoglycans [26].…”

Section: Discussionmentioning

confidence: 99%

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Hyaluronic acid-bearing lipoplexes: Physico-chemical characterization and in vitro targeting of the CD44 receptor

Wojcicki

Hillaireau

Nascimento

et al. 2012

Journal of Controlled Release

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The mechanism by which hyaluronic acid (HA)-bearing lipoplexes target the A549 lung cancer cell line was evaluated. For this purpose, cationic liposomes targeting the CD44 receptor were designed thanks to the incorporation in their composition of a conjugate between high molecular weight HA and the lipid DOPE (HA-DOPE). Liposomes containing HA-DOPE were complexed at different lipids:DNA ratios with a reporter plasmid encoding the green fluorescent protein (GFP). Diameter, zeta potential, lipoplex stability and DNA protection from nucleases have been determined.Lipids:DNA ratios of 2, 4 and 6 provided a diameter around 250 nm with a zeta potential of − 30 mV.The strength of lipids:DNA interaction and the fraction of DNA protected from enzymatic degradation increased with the lipids:DNA ratio. 2D-immunoelectrophoresis demonstrated the low capacity to activate the C3 fraction of the complement system of any of these three ratios, with and without HA-DOPE. Transfection efficiency in the presence of 0, 10 and 15% of HA-DOPE or unconjugated HA, was determined on the CD44-expressing A549 cells by flow cytometry.Lipoplexes at a lipids:DNA ratio of 2 containing 10% (w/w) of HA-DOPE were the most efficient for transfection. The maximal level of GFP expression was obtained after 6 h of incubation demonstrating a slow transfection kinetics of lipoplexes. Finally, lipoplex cellular uptake, measured indirectly by the level of transfection using flow cytometry and validated by fluorescence microscopy, was shown to be mediated by the CD44 receptor and caveolae. These results demonstrate the strong specificity of DNA targeting through the CD44 receptor using HA of high molecular weight as a ligand.

show abstract

Section: Discussionsupporting

confidence: 92%

“…This also confirms the crucial role of the receptor in the endocytosis mediated process, as shown previously by us and others [25] and [29].…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Hyaluronic acid-bearing lipoplexes: Physico-chemical characterization and in vitro targeting of the CD44 receptor

Wojcicki

Hillaireau

Nascimento

et al. 2012

Journal of Controlled Release

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“…Also, the steric hindrance effect of the endogenous HA polysaccharides around the surface of the NP would help to avoid uptake by the reticuloendothelial system, thereby prolonging the retention of NP in the joint tissue. Mizrahy et al 35 reported that the attachment of HA ranging from 6.4 to 1,500 kDa on the NP surface acts as a steric barrier to reduce clearance of the NPs by macrophages in the liver and promotes enhanced permeation and retention.…”

Section: Hyperspectral Imaging Of Np/ha Aggregatesmentioning

confidence: 99%

Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint

Kim¹,

Ho²,

Lee³

et al. 2015

IJN

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Positively surface-charged poly(lactide- co -glycolide) (PLGA)/Eudragit RL nanoparticles (NPs) were designed to increase retention time and sustain release profile in joints after intra-articular injection, by forming micrometer-sized electrostatic aggregates with hyaluronic acid, an endogenous anionic polysaccharide found in high amounts in synovial fluid. The cationic NPs consisting of PLGA, Eudragit RL, and polyvinyl alcohol were fabricated by solvent evaporation technique. The NPs were 170.1 nm in size, with a zeta potential of 21.3 mV in phosphate-buffered saline. Hyperspectral imaging (CytoViva ® ) revealed the formation of the micrometer-sized filamentous aggregates upon admixing, due to electrostatic interaction between NPs and the polysaccharides. NPs loaded with a fluorescent probe (1,1′-dioctadecyl-3,3,3′,3′ tetramethylindotricarbocyanine iodide, DiR) displayed a significantly improved retention time in the knee joint, with over 50% preservation of the fluorescent signal 28 days after injection. When DiR solution was injected intra-articularly, the fluorescence levels rapidly decreased to 30% of the initial concentration within 3 days in mice. From these findings, we suggest that PLGA-based cationic NPs could be a promising tool for prolonged delivery of therapeutic agents in joints selectively.

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“…HA has been proposed as an alternative to polyethylene glycol to improve the pharmacokinetic properties of therapeutic agents. [40][41][42] Therefore, the drug delivery system could partially evade accumulation in the liver through the decoration of HA on the nanosheets. As shown in Figure 11A(c) and B, distinct whitening at the tumor locations according to the T1-weighted MRI following the 4-hour administration was detected.…”

Section: Mri Evaluation In Vivomentioning

confidence: 99%

Manganese dioxide nanosheets-based redox/pH-responsive drug delivery system for cancer theranostic application

Hao¹,

Wang²,

Zhang³

et al. 2016

IJN

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The aim of this study was to construct redox- and pH-responsive degradable manganese dioxide (MnO 2 ) nanosheets for cancer theranostic application. The small MnO 2 nanosheets were synthesized, and then functionalized by hyaluronic acid (HA), demonstrating excellent stability and tumor-targeting ability. Cisplatin ( cis -diamminedichloroplatinum [CDDP]) was absorbed by the nanosheets through a physical action, which was designed as MnO 2 /HA/CDDP. The prepared MnO 2 /HA/CDDP formulation was able to efficiently deliver CDDP to tumor cells in vitro and in vivo, resulting in improved therapeutic efficiency. Subsequently, they were triggered by lower pH and higher level of reduced glutathione to generate Mn 2+ , enabling magnetic resonance imaging. The smart multifunctional system combining efficient magnetic resonance imaging and chemotherapy has the potential to be used as a tumor-targeting theranostic nanomedicine.

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Hyaluronan-coated nanoparticles: The influence of the molecular weight on CD44-hyaluronan interactions and on the immune response

Cited by 213 publications

References 52 publications

Hyaluronic acid-bearing lipoplexes: Physico-chemical characterization and in vitro targeting of the CD44 receptor

Hyaluronic acid-bearing lipoplexes: Physico-chemical characterization and in vitro targeting of the CD44 receptor

Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint

Manganese dioxide nanosheets-based redox/pH-responsive drug delivery system for cancer theranostic application

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