Hyaluronan-binding motif identified by panning a random peptide display library

Amemiya, Kana; Nakatani, Tatsuya; Saito, Akio; Suzuki, Atsuo; Munakata, Hiroshi

doi:10.1016/j.bbagen.2005.04.029

Cited by 25 publications

(24 citation statements)

References 38 publications

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“…Binding of the extracellular domain of ASICs to extracellular components, could functionally form a tether to physically open the channel 59. The extracellular domain of ASIC3 contains a sequence (amino acids 382–398 of mouse ASIC3, accession number Q6X1Y6, NCBI Entrez protein database) that is consistent with potential receptor for hyaluronic acid-mediated motility (RHAMM)-like binding motifs for hyaluronan 60 61. However, the current data show no binding of hyaluronan to ASIC3.…”

Section: Discussionmentioning

confidence: 54%

Acid-sensing ion channel 3 expressed in type B synoviocytes and chondrocytes modulates hyaluronan expression and release

Kolker

Walder

Usachev

et al. 2009

Annals of the Rheumatic Diseases

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Background Rheumatoid arthritis is an inflammatory disease marked by intra-articular decreases in pH, aberrant hyaluronan regulation and destruction of bone and cartilage. Acid-sensing ion channels (ASICs) are the primary acid sensors in the nervous system, particularly in sensory neurons and are important in nociception. ASIC3 was recently discovered in synoviocytes, non-neuronal joint cells critical to the inflammatory process. Objectives To investigate the role of ASIC3 in joint tissue, specifically the relationship between ASIC3 and hyaluronan and the response to decreased pH. Methods Histochemical methods were used to compare morphology, hyaluronan expression and ASIC3 expression in ASIC3+/+ and ASIC3−/− mouse knee joints. Isolated fibroblast-like synoviocytes (FLS) were used to examine hyaluronan release and intracellular calcium in response to decreases in pH. Results In tissue sections from ASIC3+/+ mice, ASIC3 localised to articular cartilage, growth plate, meniscus and type B synoviocytes. In cultured FLS, ASIC3 mRNA and protein was also expressed. In FLS cultures, pH 5.5 increased hyaluronan release in ASIC3+/+ FLS, but not ASIC3−/− FLS. In FLS from ASIC3+/+ mice, approximately 50% of cells (25/53) increased intracellular calcium while only 24% (14/59) showed an increase in ASIC3−/− FLS. Of the cells that responded to pH 5.5, there was significantly less intracellular calcium increases ASIC3−/− FLS compared to ASIC3+/+ FLS. Conclusion ASIC3 may serve as a pH sensor in synoviocytes and be important for modulation of expression of hyaluronan within joint tissue.

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Section: Discussionmentioning

confidence: 54%

Acid-sensing ion channel 3 expressed in type B synoviocytes and chondrocytes modulates hyaluronan expression and release

Kolker

Walder

Usachev

et al. 2009

Annals of the Rheumatic Diseases

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“…Additionally, a second HA binding motif, known as the link module, consists of a span of about 100 amino acids which binds HA when oriented in the correct tertiary structure [70]. A third possible binding motif is an arginine-arginine (R-R) sequence that has been shown biochemically to bind HA, but has not been thoroughly studied in full length proteins [71]. …”

Section: Ha-protein Interactionsmentioning

confidence: 99%

Hyaluronan: A simple polysaccharide with diverse biological functions

Dicker¹,

Gurski²,

et al. 2014

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Hyaluronan (HA) is a linear polysaccharide with disaccharide repeats of D-glucuronic acid and N-acetyl-D-glucosamine. It is evolutionary conserved and abundantly expressed in the extracellular matrix (ECM), on the cell surface and even inside cells. Being a simple polysaccharide, HA exhibits an astonishing array of biological functions. HA interacts with various proteins or proteoglycans to organize the ECM and to maintain tissue homeostasis. The unique physical and mechanical properties of HA contribute to the maintenance of tissue hydration, the mediation of solute diffusion through the extracellular space and the lubrication of certain tissues. The diverse biological functions of HA are manifested through its complex interactions with matrix components and resident cells. Binding of HA with cell surface receptors activates various signaling pathways that regulate cell function, tissue development, inflammation, wound healing and tumor progression and metastasis. Taking advantage of the inherent biocompatibility and biodegradability of HA, as well as its susceptibility to chemical modification, researchers have developed various HA-based biomaterials and tissue constructs with promising and broad clinical potential. In this article, we illustrate the properties of HA from a matrix biology perspective by first introducing principles underlying the biosynthesis and biodegradation of HA, as well as the interactions of HA with various proteins and proteoglycans. We next highlight the roles of HA in physiological and pathological states, including morphogenesis, wound healing and tumor metastasis. A deeper understanding of the mechanisms underlying the roles of HA in various physiological processes can provide new insights and tools for the engineering of complex tissues and tissue models.

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“…The concept of using engineered flagella as bionanotubes and their incorporation into other devices and materials was first proposed in 1990 by Fedorov. 60 The deletion and insertion tolerance of the hypervariable region has previously been exploited to allow the construction of engineered flagella that can bind to different molecules and surfaces via inserted fusion peptides, [61][62][63][64][65][66][67][68][69][70][71][72][73][74] similar in some respects to phage display systems. A major goal of our research is to demonstrate and develop flagella as a bionanotube toolkit, as previously demonstrated for the M13 virus.…”

Section: N-terminus C-terminusmentioning

confidence: 99%

Generation and Characterization of Inorganic and Organic Nanotubes on Bioengineered Flagella of Mesophilic Bacteria

Kumara

Muralidharan

Tripp³

2007

J. Nanosci. Nanotech.

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Three types of rationally designed peptide loops were genetically engineered for display on the surface of the FliTrx E. coil flagella scaffold, a type of bacterial bionanotube adapted for the multivalent display of peptide loops. The resulting three types of loop flagella fibers were used to demonstrate the feasibility of templating synthesis of inorganic nanotubes and nanoparticles and organic nanotubes. Purified flagella fibers displaying a cationic arginine-lysine loop peptide with three guanidine and three amine functional groups were used to form silica bionanotubes, using two types of silicate ion precursors. Purified flagella fibers displaying a tyrosine-serine-glycine loop peptide with six phenolic and three aliphatic hydroxyl groups were used to initiate formation of titania bionanotubes. Purified flagella fibers displaying an anionic aspartate-glutamate loop peptide with 18 carboxylate groups were used to initiate formation of polyaniline nanotubes and hydroxyapatite nanoparticles, a key component of bones. The resulting nanomaterials were mainly characterized by transmission electron microscopy and additionally by scanning electron microscopy, in the case of polyaniline nanotubes. The studies demonstrate the versatility of employing bioengineered flagella for the generation of a variety of nanoparticle arrays and nanotubes.

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Hyaluronan-binding motif identified by panning a random peptide display library

Cited by 25 publications

References 38 publications

Acid-sensing ion channel 3 expressed in type B synoviocytes and chondrocytes modulates hyaluronan expression and release

Acid-sensing ion channel 3 expressed in type B synoviocytes and chondrocytes modulates hyaluronan expression and release

Hyaluronan: A simple polysaccharide with diverse biological functions

Generation and Characterization of Inorganic and Organic Nanotubes on Bioengineered Flagella of Mesophilic Bacteria

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