Hyaluronan-based pericellular matrix: substrate electrostatic charges and early cell adhesion events

Fotia, Caterina; Messina, Grazia M. L.; Marletta, Giovanni; Baldini, Nicola; Ciapetti, G.

doi:10.22203/ecm.v026a10

Cited by 22 publications

(9 citation statements)

References 34 publications

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“…The influence of RGD, a simple peptide often used to enhance cell attachment, was also minimal. There are few direct studies of the influence of surface charge on cell attachment in the absence of, or without pre-treatment with, matrix ligands (Hamdan et al 2006;Fotia et al 2013). Thus, for the first time, we demonstrate that simply modifying the surface charge of a cellulose scaffold, by derivatisation using chemistry developed for the cloth dying industry, promotes attachment of cells (70% increase over unmodified cellulose scaffolds).…”

Section: Discussionmentioning

confidence: 68%

Surface modified cellulose scaffolds for tissue engineering

et al. 2016

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We report the ability of cellulose to support cells without the use of matrix ligands on the surface of the material, thus creating a two-component system for tissue engineering of cells and materials. Sheets of bacterial cellulose, grown from a culture medium containing Acetobacter organism were chemically modified with glycidyltrimethylammonium chloride or by oxidation with sodium hypochlorite in the presence of sodium bromide and 2,2,6,6-tetramethylpipiridine 1-oxyl radical to introduce a positive, or negative, charge, respectively. This modification process did not degrade the mechanical properties of the bulk material, but grafting of a positively charged moiety to the cellulose surface (cationic cellulose) increased cell attachment by 70% compared to unmodified cellulose, while negatively charged, oxidised cellulose films (anionic cellulose), showed low levels of cell attachment comparable to those seen for unmodified cellulose. Only a minimal level of cationic surface derivitisation (ca 3% degree of substitution) was required for increased cell attachment and no mediating proteins were required. Cell adhesion studies exhibited the same trends as the attachment studies, while the mean cell area and aspect ratio was highest on the cationic surfaces. Overall, we demonstrated the utility of positively charged bacterial cellulose in tissue engineering in the absence of proteins for cell attachment.

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Section: Discussionmentioning

confidence: 68%

Surface modified cellulose scaffolds for tissue engineering

et al. 2016

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“…Additionally, a particle exclusion assay was used to visualize cell surface HA 6, 15 . Cancer cells were plated at a concentration of 1×10 4 cells/ml into 96 well plates and incubated overnight at 37°C with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

In vitro elucidation of the role of pericellular matrix in metastatic extravasation and invasion of breast carcinoma cells

et al. 2018

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Numerous studies have demonstrated the importance of altered hyaluronan metabolism to malignant progression of multiple tumor types, including breast carcinomas. Increased hyaluronan (HA) metabolism in the stroma of primary tumors promotes activation of oncogenic signaling pathways that impact tumor initiation, growth, and invasion. Carcinoma cell synthesis and assembly of HA-rich pericellular matrices induces a stromal-independent phenotype, which is associated with cancer progression. Although the pro-tumorigenic role of stromal HA is well established, a novel but unexplored hypothesis is that carcinoma cell-associated HA pericellular matrices promote metastasis of circulating tumor cells. Here, we report the development of an in vitro assay that employs microfluidic techniques to directly measure the importance of an HA-rich pericellular matrix in the entry of carcinoma cells into ectopic sites. This model provides the capability to visualize specific steps in metastasis, which is difficult using animal models. The results show that the presence of a HA-rich pericellular matrix correlates to the invasive and metastatic potential of breast carcinoma cells. Furthermore, enzymatic removal or pharmacologic inhibition of HA synthesis significantly inhibits carcinoma cell extravasation and invasion in this model system. These results implicate pericellular HA-rich carcinoma cell associated pericellular matrices in colonization of ectopic sites by circulating tumor cells and support specific targeting of this matrix to limit metastasis in patients.

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“…These results suggest that early cell adhesion is mediated by the electrostatic interaction of cationic charges with the negatively charged pericellular matrix of the cell membrane. The importance of the pericellular matrix for early cell adhesion has also been reported for other cell types such as osteoblasts, chondrocytes or epithelial cells (Cohen et al, 2003;Fotia et al, 2013;Scrimgeour et al, 2017). RGD-integrin interaction and focal adhesion formation take place in a second step when the cell-substrate distance is in a range of 25-50 nm leading again to a doubling in adherent cells compared to the cationic gel whereas the pure RGDfunctionalized gel did not show any cell adhesive properties.…”

Section: Hydrogels As Matrices For Cell Cultivationmentioning

confidence: 60%

Hydrogels Derivatized With Cationic Moieties or Functional Peptides as Efficient Supports for Neural Stem Cells

et al. 2020

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The increasing incidence of neurodegenerative diseases such as Alzheimer's or Parkinson's disease represents a significant burden for patients and national health systems. The conditions are primarily caused by the death of neurons and other neural cell types. One important aim of current stem cell research is to find a way to replace the lost cells. In this perspective, neural stem cells (NSCs) have been considered as a promising tool in the field of regenerative medicine. The behavior of NSCs is modulated by environmental influences, for example hormones, growth factors, cytokines, and extracellular matrix molecules or biomechanics. These factors can be studied by using well-defined hydrogels, which are polymeric networks of synthetic or natural origin with the ability to swell in water. These gels can be modified with a variety of molecules and optimized with regard to their mechanical properties to mimic the natural extracellular environment. In particular modifications applying distinct units such as functional domains and peptides can modulate the development of NSCs with regard to proliferation, differentiation and migration. One well-known peptide sequence that affects the behavior of NSCs is the integrin recognition sequence RGD that has originally been derived from fibronectin. In the present review we provide an overview concerning the applications of modified hydrogels with an emphasis on synthetic hydrogels based on poly(acrylamides), as modified with either cationic moieties or the peptide sequence RGD. This knowledge might be used in tissue engineering and regenerative medicine for the therapy of spinal cord injuries, neurodegenerative diseases and traumata.

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Hyaluronan-based pericellular matrix: substrate electrostatic charges and early cell adhesion events

Cited by 22 publications

References 34 publications

Surface modified cellulose scaffolds for tissue engineering

Surface modified cellulose scaffolds for tissue engineering

In vitro elucidation of the role of pericellular matrix in metastatic extravasation and invasion of breast carcinoma cells

Hydrogels Derivatized With Cationic Moieties or Functional Peptides as Efficient Supports for Neural Stem Cells

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