Hyaluronan-based hydrogels via ether-crosslinking: Is HA molecular weight an effective means to tune gel performance?

Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication

“…Gels were investigated for the BDDE/HA content by 1 H-NMR, as previously described, with slight modifications [25]. Briefly, gels were diluted to 4 mg/mL in HCl 0.01 M and incubated for 72 h at 70 • C under stirring (400 rpm).…”

“…Sensitivity of the gels to enzymatic degradation was evaluated as previously reported with slight modification [7,8,25]. Specifically, the amount (mg/mL) of HA solubilized due to BTH action was determined as a measure of degradation.…”

“…Considering that gel-projection capacity is related to its stiffness, G' (Storage Modulus) has long been the only parameter considered in selecting the most suitable gel for the specific clinical need. A more recent approach has been extended to the whole fluido-dynamic gel behavior as well as to its sensitivity to enzymatic and radical degradation and to other properties (e.g., hydration capacity, cohesivity) [1,[5][6][7][8][14][15][16][17][18][19][20][21][22][23][24][25]. All of the latter features are known to contribute to the in vivo effect after injection and/or over time, and they are therefore generally studied to predict relative in vivo performance and to provide clinicians with valuable information to select treatments and optimize outcome [1,[5][6][7][8][14][15][16][17][18][19][20][21][22][23][24][25].…”

“…Former studies and literature report extensively analyzed volumetric gels and compared available formulations, suggesting opportunities toward design optimization, and supporting their appropriate selection and use [1,[5][6][7][8][14][15][16][17][18][19][20][21][22][23][24][25]. On the contrary, no similar studies have been carried out so far to scientifically assist this recent "skinboosting" approach.…”

Gelatin-biofermentative unsulfated glycosaminoglycans semi-interpenetrating hydrogels via microbial-transglutaminase crosslinking enhance osteogenic potential of dental pulp stem cells

“…HA, BC and gel hydrodynamic characterization was performed using the size exclusion chromatography coupled with triple detector array (SEC-TDA) system by Viscotek (Malvern) under previously reported conditions [ 39 ]. The molecular weight (M w , M n , M w /M n ), molecular size (hydrodynamic radius-R h ) and intrinsic viscosity ([η]) distributions were derived.…”