HVA22 from citrus: A small gene family whose some members are involved in plant response to abiotic stress

Ferreira, Monique Drielle Gomes; Castro, Jacqueline Araújo; Silva, Raner José Santana; Micheli, Fabienne

doi:10.1016/j.plaphy.2019.08.003

Cited by 21 publications

(37 citation statements)

References 40 publications

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“…Our RNA-seq analysis revealed that PthA4 promotes the expression of multiple stress-associated genes in kumquat. In particular, several ABA-responsive gene families, such as HVA22 , EARLY RESPONSIVE to DEHYDRATION ( ERD ) and HEAT SHOCK TRANSCRIPTION FACTOR A2 ( HSTFA2 [ 67 – 69 ]), were upregulated by PthA4 at four DPI ( S4E Fig ).…”

Section: Resultsmentioning

confidence: 99%

The immunity of Meiwa kumquat against Xanthomonas citri is associated with a known susceptibility gene induced by a transcription activator-like effector

Teper

et al. 2020

PLoS Pathog

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Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the most devastating diseases in citrus. Meiwa kumquat (Fortunella crassifolia) has shown a durable resistance against Xcc. Here, we aimed to characterize the mechanisms responsible for such a durable resistance by characterizing the transcriptional and physiological responses of Meiwa kumquat to Xcc. Inoculation of Meiwa kumquat with Xcc promoted immune responses such as upregulation of PR genes, accumulation of salicylic acid, hypersensitive response (HR)-like cell death and early leaf abscission. Hypertrophy and hyperplasia symptoms, which are known to be caused by Xcc-induction of the canker susceptibility gene LOB1 through the transcription activator-like effector (TALE) PthA4, always appear prior to the development of cell death. Mutation of pthA4 in Xcc abolished the induction of LOB1, canker symptoms, cell death, and leaf abscission and reduced the expression of PR genes in inoculated kumquat leaves without reducing Xcc titers in planta. Transcriptome analysis demonstrated that PthA4 promotes plant biotic and abiotic stress responses and the biosynthesis of abscisic acid. Transcriptional induction of LOB1 homologs in Meiwa kumquat by Xcc pthA4 mutant strains carrying a repertoire of designer TALEs promoted the elicitation of HR-like phenotype and leaf abscission, suggesting that kumquat response to Xcc is associated with upregulation of LOB1. Our study suggests a novel mechanism of plant resistance to Xanthomonas via elicitation of immune responses by upregulation of a host susceptibility gene.

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Section: Resultsmentioning

confidence: 99%

The immunity of Meiwa kumquat against Xanthomonas citri is associated with a known susceptibility gene induced by a transcription activator-like effector

Teper

et al. 2020

PLoS Pathog

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“…HVA22 was first identified as an abscisic acid (ABA)- and stress-induced gene from barley ( Hordeum vulgare ) [ 4 ]. Transmembrane domains that are critical for the proper localization and functions of HVA22 proteins, such as regulation of programmed cell death in aleurone cells and vesicular trafficking in stressed cells, are prevalent in the TB2/DP1/HVA22 domain regions of HVA22 family members [ 1 , 5 , 6 ]. To date, over 355 HVA22 homologous proteins have been identified in eukaryotes, and the TB2/DP1/HVA22 domain is highly conserved in all of them [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

“…HVA22 induced by ABA regulates seed germination and seedling growth by modulating vesicular trafficking in barley aleurone cells [ 1 , 5 ]. Plant HVA22 homologous genes responsive to environmental stresses harbor cis -regulatory elements associated with stress-related hormones and stress tolerance in their promoters [ 6 , 9 , 10 , 11 ]. The interaction between the cis elements in the HVA22 promoter and ABA- and stress-related genes has been verified in several plant species [ 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Genome-Wide Analysis and Expression Pattern Profiling of the SlHVA22 Gene Family Unravels Their Likely Involvement in the Abiotic Stress Adaptation of Tomato

Wai

Waseem²,

Cho³

et al. 2022

IJMS

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HVA22 family proteins with a conserved TB2/DP1/HVA22 domain are ubiquitous in eukaryotes. HVA22 family genes have been identified in a variety of plant species. However, there has been no comprehensive genome-wide analysis of HVA22 family genes in tomato (Solanum lycopersicum L.). Here, we identified 15 non-redundant SlHVA22 genes with three segmentally duplicated gene pairs on 8 of the 12 tomato chromosomes. The predicted three-dimensional (3D) models and gene ontology (GO) annotations of SlHVA22 proteins pointed to their putative transporter activity and ability to bind to diverse ligands. The co-expression of SlHVA22 genes with various genes implicated in multiple metabolic pathways and the localization of SlHVA22-GFP fused proteins to the endoplasmic reticulum suggested that they might have a variety of biological functions, including vesicular transport in stressed cells. Comprehensive expression analysis revealed that SlHVA22 genes were differentially expressed in various organs and in response to abiotic stress conditions. The predominant expression of SlHVA22i at the ripening stage and that of SlHVA22g, SlHVA22k, and SlHVA22l in fruits at most developmental stages suggested their probable involvement in tomato fruit development and ripening. Moreover, the transcript expression of most tomato HVA22 genes, particularly SlHVA22b, SlHVA22i, SlHVA22k, SlHVA22l, SlHVA22m, and SlHVA22n, was affected by abscisic acid (ABA) and diverse abiotic stress treatments, indicating the likely involvement of these genes in tomato abiotic stress responses in an ABA-dependent manner. Overall, our findings provide a foundation to better understand the structures and functional roles of SlHVA22 genes, many of which might be useful to improve the abiotic stress tolerance and fruit quality of tomato through marker-assisted backcrossing or transgenic approaches.

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“…HVA22-like protein is likely to be localized in ER and Golgi membranes (Guo and Ho, 2008). In particular, HVA22 proteinswere estimated to be involved in vesicular tra c in stressed cells of citrus and one of them is likely to be involved in dehydration tolerance and oxidative stress reduction (Ferreira et al, 2019).…”

Section: Involvement Of "Signal Transduction"-categorized Genes In Fr...mentioning

confidence: 99%

Identification of low temperature inducible genes of Lactuca sativa by using suppression subtractive hybridization method

Honjoh

Okano

Sasaki

et al. 2022

Preprint

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Lettuce ( Lactuca sativa ) is one of important vegetables taken as raw state. It is expected to be kept as high quality under low temperature conditions, especially freezing state. However, there are few research regarding to its molecular response to abiotic stress of lettuce. As we reported previously, lettuce plants acquire low levels of freeze tolerance. In the present paper, we have isolated 192 cDNA clones corresponding to cold induced genes of lettuce plant by using a PCR-based suppression subtractive hybridization method. Most clones were categorized into 62 distinct known genes based on homology search. Out of the corresponding genes, 45 genes were confirmed to be low-temperature-inducible with reverse transcription-qPCR. Some of the genes encoded stress-related proteins, such as late embryogenesis abundant proteins including dehydrin, which were expected to be involved in enhancement of freezing tolerance. On the other hand, some of proteins encoded by genes were suspected to be involved in suppressing the enhancement of freezing tolerance, such as vacuolar processing enzyme (VPE), adagio protein, and gigantea-like protein. In particular, VPE have been reported to be associated with program cell death, suggesting that it is negatively involved in freezing tolerance of lettuce.

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HVA22 from citrus: A small gene family whose some members are involved in plant response to abiotic stress

Cited by 21 publications

References 40 publications

The immunity of Meiwa kumquat against Xanthomonas citri is associated with a known susceptibility gene induced by a transcription activator-like effector

The immunity of Meiwa kumquat against Xanthomonas citri is associated with a known susceptibility gene induced by a transcription activator-like effector

Comprehensive Genome-Wide Analysis and Expression Pattern Profiling of the SlHVA22 Gene Family Unravels Their Likely Involvement in the Abiotic Stress Adaptation of Tomato

Identification of low temperature inducible genes of Lactuca sativa by using suppression subtractive hybridization method

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