Hv-CBF2A overexpression in barley accelerates COR gene transcript accumulation and acquisition of freezing tolerance during cold acclimation

Jeknić, Zoran; Pillman, Katherine A.; Dhillon, Taniya; Skinner, Jeffrey S.; Veisz, O.; Cuesta‐Marcos, Alfonso; Hayes, Patrick; Jacobs, Andrew K.; Chen, Tony H. H.; Stockinger, Eric J.

doi:10.1007/s11103-013-0119-z

Cited by 53 publications

(55 citation statements)

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“…1). Noteworthy, as already reported in Arabidopsis (Novillo et al 2011), self-regulated feedback loops for CBFs action are also putatively involved in Triticeae as showed by two recent studies (Soltész et al 2013;Jeknić et al 2014). Wheat TaCBF14 and TaCBF15 (from the central cluster) and barley HvCBF2A (from the proximal cluster) were overexpressed in the frost susceptible barley cultivar 'Golden Promise' and the resulting modulation in planta of other HvCBFs showed their both positive and negative influence.…”

Section: Discussionmentioning

confidence: 54%

“…Wheat TaCBF14 and TaCBF15 (from the central cluster) and barley HvCBF2A (from the proximal cluster) were overexpressed in the frost susceptible barley cultivar 'Golden Promise' and the resulting modulation in planta of other HvCBFs showed their both positive and negative influence. Moreover, Jeknić et al (2014) linked this effect to the presence/absence of CRT/DRE motifs harboured in the promoters of target CBF genes.…”

Section: Discussionmentioning

confidence: 99%

“…In fact, even though mutations within individual CBF-coding sequences were firstly proposed as causative of frost resistance variation in diploid wheat (Knox et al 2008), a differential expansion of the gene cluster together with copy number variation (CNV), has been put forward as a structural explanation for the functional role played by the CBFs at FR-2 in different genotypes. Evidences in favour of this hypothesis start to accumulate in barley and hexaploid wheat Pearce et al 2013;Jeknić et al 2014;Zhu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The corresponding COR and DHN proteins operate as effector components of the stress response, and are involved in protecting the cell against the harmful effects of frost (Thomashow 2010). Overexpression of specific CBFs from wheat and barley in the susceptible barley cultivar 'Golden Promise' accelerates COR and DHN transcript accumulation during cold acclimation and results in an improved freezing survival (Morran et al 2011;Soltész et al 2013;Jeknić et al 2014). The CBF gene family in temperate cereals is particularly large and complex, with up to 25 different members classified into ten groups that share common phylogenetic origin and similar structural features Badawi et al 2007).…”

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confidence: 99%

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Copy number variation at the HvCBF4–HvCBF2 genomic segment is a major component of frost resistance in barley

Francia

Morcia²,

Pasquariello

et al. 2016

Plant Mol Biol

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A family of CBF transcription factors plays a major role in reconfiguring the plant transcriptome in response to low-freezing temperature in temperate cereals. In barley, more than 13 HvCBF genes map coincident with the major QTL FR-H2 suggesting them as candidates to explain the function of the locus. Variation in copy number (CNV) of specific HvCBFs was assayed in a panel of 41 barley genotypes using RT-qPCR. Taking advantage of an accurate phenotyping that combined Fv/Fm and field survival, resistance-associated variants within FR-H2 were identified. Genotypes with an increased copy number of HvCBF4 and HvCBF2 (at least ten and eight copies, respectively) showed greater frost resistance. A CAPS marker able to distinguish the CBF2A, CBF2B and CBF2A/B forms was developed and showed that all the higher-ranking genotypes in term of resistance harbour only CBF2A, while other resistant winter genotypes harbour also CBF2B, although at a lower CNV. In addition to the major involvement of the HvCBF4-HvCBF2 genomic segment in the proximal cluster of CBF elements, a negative role of HvCBF3 in the distal cluster was identified. Multiple linear regression models taking into account allelic variation at FR-H1/VRN-H1 explained 0.434 and 0.550 (both at p < 0.001) of the phenotypic variation for Fv/Fm and field survival respectively, while no interaction effect between CNV at the HvCBFs and FR-H1/VRN-H1 was found. Altogether our data suggest a major involvement of the CBF genes located in the proximal cluster, with no apparent involvement of the central cluster contrary to what was reported for wheat

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Copy number variation at the HvCBF4–HvCBF2 genomic segment is a major component of frost resistance in barley

Francia

Morcia²,

Pasquariello

et al. 2016

Plant Mol Biol

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“…As the CBFs are capable of altering significant portions of the transcriptome and affecting increases in freezing tolerance (Fowler and Thomashow 2002;Vogel et al 2005;Soltész et al 2013;Jeknić et al 2014), a fundamental question raised is, what controls the CBFs. Addressing this question indicates that the diminution in FR-2 CBF transcript levels coinciding with the reproductive transition appears to be mediated by VRN-1 (Stockinger et al 2007;Dhillon et al 2010;Deng et al 2015).…”

Section: Introductionmentioning

confidence: 99%

CBF2A–CBF4B genomic region copy numbers alongside the circadian clock play key regulatory mechanisms driving expression of FR-H2 CBFs

2017

Self Cite

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The C-Repeat Binding Factors (CBFs) are DNA-binding transcriptional activators that were identified using Arabidopsis thaliana. In barley, Hordeum vulgare, a cluster of CBF genes reside at FROST RESISTANCE-H2, one of two loci having major effects on winter-hardiness. FR-H2 was revealed in a population derived from the winter barley 'Nure' and the spring barley 'Trèmois'. 'Nure' harbors two to three copies of CBF2A and CBF4B as a consequence of tandem iteration of the genomic region encompassing these genes whereas 'Trèmois' harbors single copies, and these copy number differences are associated with their transcript level differences. Here we explore further the relationship between FR-H2 CBF gene copy number and transcript levels using 'Admire', a winter barley accumulating FR-H2 CBF gene transcripts to very high levels, and a group of lines related to 'Admire' through descent. DNA blot hybridization indicated the CBF2A-CBF4B genomic region is present in 7-8 copies in 'Admire' and is highly variable in copy number across the lines related to 'Admire'. At normal growth temperatures transcript levels of CBF12, CBF14, and CBF16 were higher in lines having greater CBF2A-CBF4B genomic region copy numbers than in lines having fewer copy numbers at peak expression level time points controlled by the circadian clock. Chromatin immunoprecipitation indicated CBF2 was at the CBF12 and CBF16 promoters at normal growth temperatures. These data support a scenario in which CBF2A-CBF4B genomic region copy numbers affect expression of other FR-H2 CBFs through a mechansim in which these other FR-H2 CBFs are activated by those in the copy number variable unit.

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Expand the Survival Limits of Crop Plants Under Cold Climate Region

Sareen

Joshi

2023

Global Climate Change and Plant Stress Management

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Hv-CBF2A overexpression in barley accelerates COR gene transcript accumulation and acquisition of freezing tolerance during cold acclimation

Abstract: C-Repeat Binding Factors (CBFs) are DNAbinding transcriptional activators of gene pathways imparting freezing tolerance. Poaceae contain three CBF subfam-

Cited by 53 publications

References 60 publications

Copy number variation at the HvCBF4–HvCBF2 genomic segment is a major component of frost resistance in barley

Copy number variation at the HvCBF4–HvCBF2 genomic segment is a major component of frost resistance in barley

CBF2A–CBF4B genomic region copy numbers alongside the circadian clock play key regulatory mechanisms driving expression of FR-H2 CBFs

Expand the Survival Limits of Crop Plants Under Cold Climate Region

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