Humoral immune response to HTLV-1 basic leucine zipper factor (HBZ) in HTLV-1-infected individuals

Abstract

Enose-Akahata et al.: Humoral immune response to HTLV-1 basic leucine zipper factor (HBZ) in HTLV-1-infected individuals.

Reevaluation of confirmatory tests for human T‐cell leukemia virus Type 1 using a luciferase immunoprecipitation system in blood donors

“…The frequency of the HBZ antibody was 0.88% (1/114) among the screeningpositive donors and 2.63% (1/38) among the confirmatory test-positive donors. Enose-Akahata and coworkers 11 reported that 10.34% (15 in 145) of asymptomatic HTLV-1 carriers exhibited antibody responses against the HBZ protein.…”

“…Figure 1B shows the luciferase enzyme activities (indicated as RLUs) of the lysates of cells transfected with each of the vectors. We also constructed a plasmid that encoded the HBZ protein fused with Renilla luciferase at the N-terminus of HBZ (RLucHBZ), as reported previously, 11 to examine whether the antibody responses were affected by the order of the HBZ and Renilla luciferase proteins. The expression level of RLucHBZ was slightly less than that of HBZRLuc ( Fig.…”

“…Recently, HBZ antibody responses were reported for the first time in both asymptomatic HTLV-1 carriers and patients with ATL or HAM/TSP in two cohorts that comprised multiple ethnicities other than Japanese. 11 We also examined the test plasma samples collected in Japan by LIPS for antibodies against HBZ. Among 114 HTLV-1 screening-positive plasma samples, we detected a single case of HBZ positivity in Group IV ( Fig.…”

Effect of Teriflunomide on Cells From Patients With Human T-cell Lymphotropic Virus Type 1–Associated Neurologic Disease

“…Lymphoproliferation assay using carboxyfluorescein succinimidyl ester (CFSE) (CellTrace CFSE cell proliferation kit; Invitrogen) was performed as described previously. 18 PBMCs were labeled with CFSE, according to the manufacturer's instruction, and plated at 3 × 10 5 cells/well in 96-well round bottom microplates with defined concentrations of teriflunomide (0, 25, 50, and 100 μM). After culture for 4 and 5 days, the cells were stained with antibodies against CD3, CD4, CD8, and CD25 (all from BD Biosciences).…”

Conference Highlights of the 16th International Conference on Human Retrovirology: HTLV and Related Retroviruses, 26–30 June 2013, Montreal, Canada

“…Enose-Akahata et al evaluated the immune response against HBZ in HTLV-I-infected individuals [ 122 ]. The immunoreactivity for HBZ was detected in subsets of all HTLV-I-infected individuals, but did not discriminate between asymptomatic carrier, ATLL and HAM/TSP.…”