Humoral Immune Response of Ayu, Plecoglossus altivelis to Glugea plecoglossi (Protozoa: Microspora).

Kim, Jeong-Ho; Yokoyama, Hiroshi; Ogawa, Kazuo; Takahashi, Sei; Wakabayashi, Hisatsugu

doi:10.3147/jsfp.31.215

Cited by 15 publications

(13 citation statements)

References 17 publications

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“…This suggests that a specific humoral immune response of E. akaara was elicited by infection with G. epinephelusis and the intensity of the infection was not related to the antibody level in naturally infected grouper using intact mature spores as a coated antigen. It is similar to that reported by Kim et al (1996).…”

Section: Disscussionsupporting

confidence: 92%

“…Much work has been done to determine the immune response of fish against microsporidians, and 2 distinct views have emerged. Some authors believe that fish infected by microsporidians can generated a specific immune response against the pathogen (Leiro et al 1993, Hung et al 1996, Kim et al 1996. However, it was also widely accepted that the parasite can evade the host's immune defense mechanism by mimicing or adsorbing host antigens, which can result in immunosuppression of the host for other pathogens (Laudan et al 1986, 1987, Wongtavatchai et al 1995, Kim et al 1998.…”

Section: Disscussionmentioning

confidence: 99%

“…Although immune responses of fish against microsporidians remain enigmatic (Laudan et al 1986, 1987, Woo 1992, Leiro et al 1993, Wongtavatchai et al 1995, Kim et al 1996, Pomport-Castillon et al 1999, specific immune responses of fish, such as humoral, mucous and cellular immune responses, have been demonstrated in some species (Leiro et al 1993, Hung et al 1996, Kim et al 1996. In the case of Glugea epinephelusis, immune responses of grouper against the infection, however, are still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Humoral immune responses of the grouper Epinephelus akaara against the microsporidium Glugea epinephelusis

Zhang

Wu²,

Wu³

et al. 2005

Dis. Aquat. Org.

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The humoral immune responses of grouper Epinephelus akaara to a natural infection with Glugea epinephelusis was studied by ELISA utilizing intact mature spores as the coated antigen. Results showed that a specific humoral immune response was elicited, but the intensity of infection (in terms of the number of cysts) was not related to the antibody level in naturally infected hosts. The differences in the antigenicity of intact mature spores and soluble spore proteins derived from cracked mature spores were also analyzed. Results suggested that similar antigen epitopes existed between the 2 groups. Additionally, antigen component patterns and the distribution of antigen with immunogenicity were investigated by using the western blot and the immunofluorescent antibody technique (IFAT). The new parasitic microsporidium has specific polypeptide patterns comparable to the reported fish microsporidians. The main antigenic substances are concentrated on the surface of spores, and are mostly located on the anterior and posterior end of the spore bodies. Most surface components of the G. epinephelusis spores are soluble. The potential role of the surface components in initiating infection was also discussed. KEY WORDS: Glugea epinephelusis · Microsporidia · Epinephelus akaara · Humoral immune responses · antigenicityResale or republication not permitted without written consent of the publisher

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Section: Disscussionsupporting

confidence: 92%

Section: Disscussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Humoral immune responses of the grouper Epinephelus akaara against the microsporidium Glugea epinephelusis

Zhang

Wu²,

Wu³

et al. 2005

Dis. Aquat. Org.

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“…In some cases, the antibody (Ab) response is well established and it has a protective role, while in other situations, the humoral response is not protective or it is depressed [65]. A few studies have established that fish can produce specific Ab in response to microsporidians.…”

Section: Humoral Responsementioning

confidence: 99%

Fish microsporidia: Immune response, immunomodulation and vaccination

Rodríguez-Tovar

Markham

2011

Fish & Shellfish Immunology

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“…Infection with Glugea plecoglossi is thought to be endemic in ayu from Lake Biwa (Takahashi, 1980 ELISA ELISA was conducted and antibody units were calcu lated from the means of the optical density as described previously (Kim et al, 1996).…”

mentioning

confidence: 99%

Elevated Water Temperature Treatment of Ayu Infected with Glugea plecoglossi: Apparent Lack of Involvement of Antibody in the Effect of the Treatment.

et al. 1997

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The possibility of antibody-mediated effect of elevated water temperature treatment of Glugea-infected ayu was investigated by challenge test and ELISA.The treatment effectively reduced the prevalence and intensity of infection.In naturally infected fish, the prevalence of infection was significantly decreased by elevated water temperature treatment.The treatment was more effective in naturally infected fish treated thrice than those treated twice, as demonstrated by the smaller number of cysts in the former group of fish than in the latter. The antibody levels were also significantly increased by the treatment in naturally infected fish. However, in the experiment using unifected fish, no significant increase in the antibody levels by the treatment was seen, though the prevalence of infection was significantly decreased by the treatment. Through the challenge test, it was clear that the effect was temporary, and protective immunity was not confirmed by the treatment. These results sug gest that the effect may not be antibody-mediated. Thus it is recommended that the elevated water temperature treatment be repeated and applied with continuous monitoring of reinfection.

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Humoral Immune Response of Ayu, Plecoglossus altivelis to Glugea plecoglossi (Protozoa: Microspora).

Cited by 15 publications

References 17 publications

Humoral immune responses of the grouper Epinephelus akaara against the microsporidium Glugea epinephelusis

Humoral immune responses of the grouper Epinephelus akaara against the microsporidium Glugea epinephelusis

Fish microsporidia: Immune response, immunomodulation and vaccination

Elevated Water Temperature Treatment of Ayu Infected with Glugea plecoglossi: Apparent Lack of Involvement of Antibody in the Effect of the Treatment.

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