Humoral immune response in hens naturally infected withSalmonellaEnteritidis against outer membrane proteins and other surface structural antigens

Abstract

-A simple procedure for obtaining surface exposed antigens of Salmonella Enteritidis is described. A heat treatment of whole bacteria in saline solution induced the release of small membrane vesicles containing outer membrane components as well as surface appendage components, such as fimbriae and flagellin. The characterization of the structural components of this extract, called HE, was established by SDS-PAGE and immunoblotting using polyclonal and monoclonal specific antibodies. Five major groups of proteins were identified: flagellin, porins, OmpA, SEF21 and SEF14 fimbriae. The immunogenicity of these proteins was studied by immunoblotting with serum samples from naturally infected hens. Flagellin, porins, OmpA, SEF14 and SEF21 fimbriae were immunogenic in the S. Enteritidis infected hens (frequency of reactants: 47.3, 97.3, 64.7, 50.0 and 60.8%, respectively); porins also reacted with sera from non infected hens (66.7%). The immunogenicity of these antigens in infected birds provide promise that they may serve as components of an effective subcellular vaccine for poultry salmonellosis. salmonellosis / immunoblotting / immunogenicity / surface antigens / hens

Evaluation of the protective efficacy of <i>Salmonella</i> Gallinarum 9R strain vaccine against <i>Salmonella</i> strains isolated from cases suspected of salmonellosis outbreaks in poultry farms in central Ethiopia

“…The cross protection by Salmonella Gallinarum, 9R strain vaccine observed in the current study is due to the fact that Salmonella Gallinarum, Salmonella Pullorum and Salmonella Enteritidis are classified as group D Salmonella (Kauffman-White scheme) implying that they share similar antigenic structure (O-antigens 1, 9 and 12) (Ochoa-Repa´raz et al, 2004;Forshell and Wierup, 2006). The higher virulence of the wild strain of S. Gallinarum as observed in the challenge experiment in non-vaccinated chicken might be associated with its immunogenicity (Wigley et al, 2005).…”

Identification of immunoreactive antigens in membrane proteins enriched fraction from Francisella tularensis LVS

“…The lipoproteins were successfully used as the potential protective antigens against some pathogens [22,23], thus they represent interesting targets for the vaccine development. The immunogenicity of OmpA protein has recently been reported in other bacteria [24,25]. Additional lipoprotein, FTT1591, is similar to lipoprotein VacJ that is required for intercellular spreading of shigellae [26].…”

Proteomic analysis of Flavobacterium psychrophilum cultured in vivo and in iron-limited media

“…Considering the surface exposure and increased spot intensities in vivo, these proteins are promising candidates for further investigation. OmpA and OmpA-like proteins have been identified as vaccine candidate antigens (Ochoa-Reparaz et al 2004, Kurupati et al 2006) and appear protective in other systems (Ross et al 2004).…”