Humoral and Cellular Immunity to Candida albicans in Patients with Bronchial Asthma.

Abstract

Delayed cutaneous reactivity to Candida albicans (C. albicans) and PPD (purified protein derivative) was examined in 52 patients with bronchial asthma in relation to the production of specific IgG4 antibodies against the antigen. 1. The frequency of a positive, immediate skin reaction to C. albicans was similar among the five age groups, ranging from 60.0% to 66.7%. 2. The incidence of a positive delayed skin reaction to C. albicans was lower in patients between the ages of 10 and 30 and tended to decrease with aging in the patients over the age of 51. 3. A delayed skin reaction to PPD was positive in patients between 31 and 50 with a higher incidence; this incidence decreased in patients over age 51. 4. The level of C. albicans-specific IgG4 antibodies was significantly higher (26.7 u/ml) in patients with a negative delayed skin reaction to the antigen than in those with a positive reaction (5.9u/ml) (p <0.001). There was no correlation between delayed skin reaction to PPD and production of specific IgG4 antibodies. (Internal Medicine 31: 766-769, 1992)

Lactoferrin Peptide Increases the Survival of Candida albicans - Inoculated Mice by Upregulating Neutrophil and Macrophage Functions, Especially in Combination with Amphotericin B and Granulocyte-Macrophage Colony-Stimulating Factor

“…C. albicans blastoconidia were labeled with 51 Cr (Na 2 CrO 4 ) for 1 h at 37°C at a concentration of 100 Ci per 10 8 cells. The blastoconidia were then washed three times and used as the targets.…”

“…The blastoconidia were then washed three times and used as the targets. The effectors were suspended in RPMI 1640 medium supplemented with 2% fetal bovine serum, and the effectors, neutrophils or macrophages, were mixed with the 51 Cr-labeled blastoconidia to give an effector/target ratio of 1:10 in a final volume of 0.2 ml/flatbottom well. The mixtures were then incubated for 4 h at 37°C, and the isotope activity in 0.1 ml of the supernatant from each well was counted with a gamma counter.…”

“…Peritoneal neutrophils and macrophages were obtained from mice as described in the legend to Fig. 2, and the phagocytes obtained were incubated with 51 Cr-2 labeled blastoconidia to give an effector/target ratio of 1:10 for 4 h at 37°C. Killing activity was assayed as described in Materials and Methods.…”

Mineral water inhalations for bronchial asthma: a meta-analysis

“…Thus, the remaining 14 articles for our analysis were written in Russian, Japanese, and English and were conducted in Russia, Japan, and Italy (Tables 3 and 4; See: Supplementary materials). [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][41][42][43][44][45] More information about the included studies is given in Tables 3 and 4 (See: Supplementary materials): the set of data included the following indicators: researcher's name, year of publication, description of key points of the study design (randomization, placebo), number of participants in each 32,46 group; description of the mineral water, quantity, application method, period of application; the values of the indicators of the respiratory function before and after the use of mineral water. As a measure of the effect, the standardized difference in mean values (Hedge's g) and 95% confidence intervals were calculated using a random effects model.…”