Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations

Leal, Élida Aparecida; Moreira, Jôsimar Dornelas; Nunes, Fernanda Freire Campos; Souza, Larissa R.; Martins, Janaina M.; Toledo, Vicente de Paulo Coelho Peixoto de; Almeida, Alzira Maria Paiva de; Guimarães, Talita Antunes

doi:10.1016/j.bjid.2017.09.001

Cited by 2 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Бактерии выращивали на агаре Хоттингера (рН 7,2) в тече ние 48 часов при температуре 28 о С и инактиви ровали нагреванием при 60 о С в течение 80 мин. В опытные и контрольные образцы крови (100 мкл) добавляли соответственно по 10 мкл УЗДК или стерильного 0,9% изотонического раствора натрия хлорида для выявления возможной спонтанной ак тивации лимфоцитов [10].…”

Section: материалы и методыunclassified

“…Всё это свидетельствует, что для оценки у лю дей клеточного противочумного иммунитета в си стеме in vitro продолжается поиск, как новых информативных клеточных маркеров, так и более эффективных специфических стимуляторов им мунного ответа. В случае применения в качестве стимулятора ультразвукового дезинтеграта кле ток Y. pestis, выращенных при температуре 28 о С (общая фракция антигенов чумного микроба), метод проточной цитометрии регистрировал в те стах in vitro более интенсивную специфическую активацию Тклеток памяти иммунных лаборатор ных животных, чем при использовании очищенно го препарата капсульного антигена [10]. Однако с клетками крови человека такие исследования не проводились.…”

Section: Introductionunclassified

See 1 more Smart Citation

Activation of T-helper in vitro under the Influence of Yersinia pestis Antigens in People Vaccinated against the Plague

Кожевников

Kravtsov

Кудрявцева

et al. 2022

Epidemiology and Vaccinal Prevention

View full text Add to dashboard Cite

Relevance. The search for informative markers for assessing the immunological efficacy of a live plague vaccine remains an urgent scientific task. The goal of the studyAims was to make a comparative assessment of the level helper T-cells activation by CD69 and HLA-DR markers, in people, vaccinated against plague, in an in vitro test, using the disintegrated by ultrasonic Y. pestis cells, grown at temperature 28 °C as a specific stimulator.Materials & Methods. A cytofluorimetric analysis of blood samples of 45 individuals vaccinated against the plague was carried out. The dependence of the result of cytological analysis of the two studied cell markers on the immunoregulatory index (IRI) of the vaccinated donor at the time of vaccination was revealed.Results. It was found that for newly vaccinated individuals, the T-helpers were more intensive and prolonged by the early activation marker, while for those revaccinated with the immunoregulatory index, at the time of vaccination more than 1.5, the more intense cellular response was by the late activation marker.Conclusions. Thus, the possibility of quantitative evaluation of the immunological efficacy of vaccination against plague, based on the identification of lymphocyte activation markers when stimulated with a specific antigen, is confirmed.

show abstract

Section: материалы и методыunclassified

Section: Introductionunclassified

Activation of T-helper in vitro under the Influence of Yersinia pestis Antigens in People Vaccinated against the Plague

Кожевников

Kravtsov

Кудрявцева

et al. 2022

Epidemiology and Vaccinal Prevention

View full text Add to dashboard Cite

show abstract

Influence of immunomodulation on intracellular cytokine expression by spleen T-helpers of mice immunized by Yersinia pestis EV NIIEG

Клюева¹,

Goncharova²,

Kravtsov³

et al. 2021

Journal of microbiology, epidemiology and immunobiology

View full text Add to dashboard Cite

Aim. To characterize the intracellular expression of cytokines by spleen T-helpers and the spontaneous production of cytokines in the blood of BALB/c mice immunized with Yersinia pestis EV NIIEG against the background of immunomodulation.Materials and methods. Intracellular expression of CD4+IFN-γ+, CD4+IL-4+, CD4+IL-17+ was determined in mice spleen cell suspensions by flow cytometry, IFN-γ and IL-10 were measured in ELISA in blood supernatants on day 3 and day 21 after the immunization with Y. pestis EV against the background of immunomodulation. On day 21 after the immunization animals were infected by Y. pestis 231 at a dose of 400 LD50.Results. Differences in cytokine response to studied drugs, correlated with CD4+IFN-γ+ levels in animals, were identified. On day 3, a significant decrease in CD4+IFN-γ+ was observed in response to Y. pestis EV and to recombinant gamma interferon (Ingaron). A significant increase in CD4+IFN-γ+ was detected in response to vaccine strain administered with azoximer bromide (Polyoxidonium). Intracellular expression of IFN-γ, IL-4 and IL-17 increased on day 21by an average of 2,3 times when immunomodulators were used in the immunization schedule. In addition, on day 21 a significant (p ˂ 0.05) increase in the proportion of T-helpers expressing IFN-γ, as well as in level of spontaneous IFN-γ production in blood supernatants was observed only in animals immunized by schedules that included immunomodulators. After the challenge with Y. pestis 231 of animals previously immunized by schedules that included Polyoxidonium, the correlation analysis confirmed the association (r = 0,94; p = 0,0004) of mice survival with intensity of CD4+IFN-γ+ expression.Conclusion. The data obtained confirm the effectiveness of Polyoxidonium application in experimental animal Y. pestis EV immunization schedule and the usefulness of intracellular cytokine expression measurement for assessment of the level of protection following the immunization.

show abstract

Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations

Abstract: The results showed that F1 protein is not an immunogenic T cell antigen, although the YP total extract (40μg dose) favoured CD8 T cell-mediated cellular immunity.

Cited by 2 publications

References 24 publications

Activation of T-helper in vitro under the Influence of Yersinia pestis Antigens in People Vaccinated against the Plague

Activation of T-helper in vitro under the Influence of Yersinia pestis Antigens in People Vaccinated against the Plague

Influence of immunomodulation on intracellular cytokine expression by spleen T-helpers of mice immunized by Yersinia pestis EV NIIEG

Contact Info

Product

Resources

About