Humoral and cell-mediated immunity following vaccination with synthetic Candida cell wall mannan derived heptamannoside–protein conjugate

Effect of Branched α‐Oligomannoside Structures on Induction of Anti‐Candida Humoral Immune Response

“…Moreover, the administration route of secondary booster injection of M6-BSA conjugate significantly affected the intensity of mannan-specific humoral immune response giving priority to sc route of administration. This observation is inconsistent with our previously published results with linear heptamannoside-BSA conjugate [14] favouring ip administration route conferring higher antibody response. Due to obtained results, we can assume oligomannoside structure-dependent difference in induced humoral immune response.…”

“…Our previously published results revealed the ability of linear a-1,2-linked mannooligomers conjugates to induce antibodies elevating candidacidal activity of leucocytes [13,14]. The results presented here are a continuation of the immunomodulatory properties assessment of a-mannoside BSA-based glycoconjugates.…”

“…Peripheral blood leucocytes population was obtained from the heparinized complete peripheral blood of mice as described before [14]. Briefly, polymorphonuclear cells (PMN) were isolated by Ficoll-Urografin gradients following dextran sedimentation of erythrocytes and finally adjusted to 1 9 10 6 cells/ml in RPMI 1600.…”

“…Next, C. albicans cells samples were washed with PBS and incubated with isolated PMN (1 9 10 6 cells/ml), to obtain target cells to effector cells ratio 5:1, for 60 minutes at 37°C. After incubation, PMN were lysed with sodium deoxycholate [13,14,21]. Propidium iodide (PI, 0.02 lg/ml, redistilled water, Sigma) and fluorescein diacetate (FDA, 5 mg/ml stock solution in acetone, 50 lg/ml, redistilled water, Lachema) staining was carried out by incubating 100 ll of the Candida suspension with 50 ll of PI and 50 ll of FDA for 30 min at room temperature in darkness.…”

“…Our previously published studies revealed that antibodies induced by synthetic oligomannoside -BSA conjugates -had the capacity to induce the candidacidal activity in vitro [13,14]. Relative efficiency of prepared a-1,6-branched oligomannoside -BSA conjugates to induce production of potentially protective antibodies with capacity to enhance C. albicans opsonophagocytic killing by polymorphonuclear cells (PMN) -was analysed and compared with previously obtained results with conjugates containing linear mannooligosaccharides.…”